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Mouse IFN-gamma Antibody

Catalog #: MAB785
20 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB785-SP
MAB785-100
MAB785-500
Detection of Mouse IFN-gamma by ELISA
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Citations (20)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFN-gamma Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IFN-gamma in ELISAs. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) C10, rmG-CSF, rmGM‑CSF, recombinant human IFN‑ gamma, rmIL-1 alpha, rmIL-1 beta, rmIL-2, rmIL-3, rmIL-4, rmIL-5, rmIL-6, rmIL-7, rmIL-9, rmIL-10, rmIL-10 R, rmIL-12, rmIL-13, rmJE, rmKC, rmLIF, rmM-CSF, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-2, rmSCF, rmTNF-alpha, rmTpo, and rmVEGF.
Source
Monoclonal Rat IgG2A and IgG2B Cocktail Clone # 37801/37875
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IFN-gamma
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Mouse IFN-gamma Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IFN‑ gamma Biotinylated Antibody (Catalog # BAF485)

Standard: Recombinant Mouse IFN-gamma Protein (Catalog # 485-MI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA Detection of Mouse IFN-gamma by ELISA View Larger

Detection of Mouse IFN-gamma by ELISA A27L-specific cell-mediated immune response against recombinant VVWR determined by ELISPOT analysis.(A) Each group consisted of four female Balb/C mice immunized three times, two weeks apart via intramuscular injection as follows: Naïve (negative control), pVax1 (empty vector backbone control), pA27L (VVWR envelope protein), pA27L+I (VVWR envelope protein + imiquimod), pA27L + R (VVWR envelope protein + resiquimod), pA27LOPT (optimized sequence of the A27L gene) and pA27LOPT+I (the optimized sequence of A27L + imiquimod). (B) Identification of A27L dominant epitopes. Antigen-specific IFN-gamma ELISPOT in splenocytes from mice (immunized three times, two weeks apart via intramuscular injection) in response to VVWR A27 individual 3-mer overlapped 15-mer overlapping peptides. Each experiment was performed three times and the immune responses among groups of mice are presented as the mean ± standard error of the mean (SEM). A p value of less than 0.05 was considered significant. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25875833), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. It shares 86% amino acid (aa) sequence identity with rat IFN-gamma, 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine and rhesus IFN‑ gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Citations for Mouse IFN-gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Depletion of Foxp3+ regulatory T cells augments CD4+ T cell immune responses in atherosclerosis-prone hypercholesterolemic mice
    Authors: K Kasahara, N Sasaki, HZ Amin, T Tanaka, S Horibe, T Yamashita, KI Hirata, Y Rikitake
    Heliyon, 2022-07-19;8(7):e09981.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. Immunological characteristics of MAV/06 strain of varicella-zoster virus vaccine in an animal model
    Authors: D Shin, Y Shin, E Kim, H Nam, H Nan, J Lee
    Oncogene, 2022-06-03;23(1):27.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Capture
  3. Characterization of the immune response elicited by the vaccinia virus L3 protein delivered as naked DNA
    Authors: M Ramírez, S Santos, O Martínez, R Rodríguez, E Miranda, WD Ramos-Pere, M Otero
    Vaccine, 2018-03-07;36(15):2049-2055.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Capture
  4. Vaccination with a codon-optimized A27L-containing plasmid decreases virus replication and dissemination after vaccinia virus challenge
    Authors: O Martínez, A Bravo Cruz, S Santos, M Ramírez, E Miranda, J Shisler, M Otero
    Vaccine, 2017-06-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)
  5. Potentiation of T Cell Stimulatory Activity by Chemical Fixation of a Weak Peptide-MHC Complex
    Authors: I Hwang, K Kim, S Choi, M Lomunova
    Mol. Cells, 2017-01-31;40(1):24-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development (Capture)
  6. Tenascin-C deficiency protects mice from experimental autoimmune encephalomyelitis
    Authors: Miljana Mom?ilovi?
    J. Neuroimmunol, 2016-12-07;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  7. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
    Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
    J. Allergy Clin. Immunol., 2016-10-26;139(5):1608-1620.e3.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  8. Immunomodulator-based enhancement of anti smallpox immune responses.
    Authors: Martinez O, Miranda E, Ramirez M, Santos S, Rivera C, Vazquez L, Sanchez T, Tremblay R, Rios-Olivares E, Otero M
    PLoS ONE, 2015-04-13;10(4):e0123113.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)
  9. An alphavirus-based adjuvant enhances serum and mucosal antibodies, T cells, and protective immunity to influenza virus in neonatal mice.
    Authors: Khalil S, Tonkin D, Snead A, Parks G, Johnston R, White L
    J Virol, 2014-06-04;88(16):9182-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)
  10. CD8(+) cells regulate the T helper-17 response in an experimental murine model of Sjogren syndrome.
    Authors: Zhang X, Schaumburg C, Coursey T, Siemasko K, Volpe E, Gandhi N, Li D, Niederkorn J, Stern M, Pflugfelder S, de Paiva C
    Mucosal Immunol, 2013-09-11;7(2):417-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)
  11. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
    Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
    Eur. J. Immunol., 2012-05-01;42(5):1250-60.
    Species: Mouse
    Sample Types: Pleural Lavage Fluid
    Applications: ELISA Development
  12. Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques.
    Authors: Kraynyak KA, Kutzler MA, Cisper NJ, Khan AS, Draghia-Akli R, Sardesal NY, Lewis MG, Yan J, Weiner DB
    Vaccine, 2010-02-23;28(8):1942-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  13. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  14. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009-03-13;11(4):524-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  15. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008-04-03;125(2):272-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  16. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008-03-19;112(2):362-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  17. DR5 activation of caspase-8 induces DC maturation and immune enhancement in vivo.
    Authors: Chattergoon MA, Muthumani K, Tamura Y, Ramanathan M, Shames JP, Saulino V, Robinson TM, Montaner LJ, Weiner DB
    Mol. Ther., 2007-12-18;16(2):419-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development
  18. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
    Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
    J. Immunol., 2006-12-15;177(12):8650-7.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
    Applications: ELISA Development
  19. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  20. Nerve growth factor induces increased airway inflammation via a neuropeptide-dependent mechanism in a transgenic animal model of allergic airway inflammation.
    Authors: Quarcoo D, Schulte-Herbruggen O, Lommatzsch M, Schierhorn K, Hoyle GW, Renz H, Braun A
    Clin. Exp. Allergy, 2004-07-01;34(7):1146-51.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development

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