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Mouse IFN-alpha / beta  R2 PE-conjugated Antibody

Catalog #: FAB1083P
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1083P
Detection of IFN-alpha / beta R2 in A20 Mouse Cell Line by Flow Cytometry.
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Citations (3)
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Mouse IFN-alpha / beta  R2 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IFN‑ alpha / beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta, rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta  R2
Ser22-Ala239 (Lys160Asn)
Accession # Q9D1R7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IFN-a/ beta R2 antibody in A20 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IFN-alpha / beta R2 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IFN-a/ beta R2 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1083P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IFN-alpha/beta R2

IFN-alpha / beta R2 belongs to the type II cytokine receptor family. It complexes with IFN-alpha / beta R1 to form the signaling receptor complex for the family of alpha and beta IFN subtypes. By alternative splicing, IFN-alpha / beta R2 can exist as a secreted soluble protein or as a type I membrane protein.

Long Name
Interferon alpha/beta Receptor 2
Entrez Gene IDs
3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)
Alternate Names
beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta R2; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFN-aR2; IFNARB; IFNbR2; IFN-bR2; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2

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Citations for Mouse IFN-alpha / beta  R2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Depression compromises antiviral innate immunity via the AVP-AHI1-Tyk2 axis
    Authors: HG Zhang, B Wang, Y Yang, X Liu, J Wang, N Xin, S Li, Y Miao, Q Wu, T Guo, Y Yuan, Y Zuo, X Chen, T Ren, C Dong, J Wang, H Ruan, M Sun, X Xu, H Zheng
    Cell Research, 2022-07-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. JAK1 Inhibition Blocks Lethal Immune Hypersensitivity in a Mouse Model of Down Syndrome
    Authors: KD Tuttle, KA Waugh, P Araya, R Minter, DJ Orlicky, M Ludwig, Z Andrysik, MA Burchill, BAJ Tamburini, C Sempeck, K Smith, R Granrath, D Tracy, J Baxter, JM Espinosa, KD Sullivan
    Cell Rep, 2020-11-17;33(7):108407.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  3. Mycobacterium tuberculosis Inhibits Autocrine Type I IFN Signaling to Increase Intracellular Survival
    Authors: DA Banks, SE Ahlbrand, VK Hughitt, S Shah, KD Mayer-Barb, SN Vogel, NM El-Sayed, V Briken
    J. Immunol., 2019-03-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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