Mouse IFN-alpha Antibody

Catalog #: 32100-1
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
32100-1

All IFN-alpha Antibodies

Product Details

Citations (3)

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFN-alpha Antibody Summary

Species Reactivity

Mouse

Specificity

Mouse IFN-alpha

Source

Polyclonal Rabbit Serum

Formulation

Neat serum diluted in phosphate buffered saline (PBS)

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

One Neutralization Unit (NU) is the amount of antiserum required to neutralize one unit of mouse interferon alpha (Mu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay using L929 cells as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758] with the exception that EMCV was used as the challenge virus. In this antiviral assay for interferon, about 5 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Mu-IFN-alpha provided by the National Institutes of Health [Ga02-901-511). This material is prepared specifically for effective neutralization of Mu-IFN-alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For
long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the
contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers
containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name

Interferon alpha

Entrez Gene IDs

3440 (Human)

Alternate Names

IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

Product Datasheets

Product Datasheet

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SDS

Related Research Areas

Citations for Mouse IFN-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology
Authors: Veridiana Ester Dias de Barros, Fabiano P. Penharvel, Juliana Helena Chávez, Rafael Freitas de Oliveira França, Luciano Mariguela, Rafael Freitas de Oliveira França et al.
The American Journal of Tropical Medicine and Hygiene
Identification of Toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells.
Authors: von Buttlar H, Siegemund S, Buttner M, Alber G
PLoS ONE, 2014-08-29;9(8):e106188.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression.
Authors: Joshi VD, Kalvakolanu DV, Chen W, Zhang L, Kang TJ, Thomas KE, Vogel SN, Cross AS
J. Interferon Cytokine Res., 2006-10-01;26(10):739-47.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development