Mouse GRIN1/NMDAR1 Antibody

Catalog #: MAB10655 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10655-100
MAB10655-SP

GRIN1/NMDAR1 in NS0 Mouse Cell Line Transfected with Mouse GRIN1/NMDAR1.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse GRIN1/NMDAR1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GRIN1/NMDAR1 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 1031915

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GRIN1/NMDAR1
Met1-Gln559

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed NS0 mouse myeloma cell line transfected with mouse GRIN1/NMDAR1

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

NS0 cells transfected with Mouse GRIN1/NMDAR1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GRIN1/NMDAR1 in NS0 Mouse Cell Line Transfected with Mouse GRIN1/NMDAR1. GRIN1/NMDAR1 was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse GRIN1/NMDAR1 (positive staining) and NS0 mouse myeloma cell line (wild type, negative control) using Rat Anti-Mouse GRIN1/NMDAR1 Monoclonal Antibody (Catalog # MAB10655) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Intracellular Staining by Flow Cytometry

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Detection of Mouse GRIN1/NMDAR1 in NS0 cells transfected with Mouse GRIN1/NMDAR1 by Flow Cytometry. NS0 cells transfected with mouse GRIN1/NMDAR1 (filled histogram) or irrelevant protein (open histogram) were stained with Rat Anti-Mouse GRIN1/NMDAR1 Monoclonal Antibody (Catalog # MAB10655) or Rat IgG2A Isotype Control Antibody (MAB006, data not shown) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRIN1/NMDAR1

Grin-1 is a critical component of NMDA receptors. Expressed in the brain, these components play a key role in plasticity of synapses, which is believed to underlie memory and learning. Missense variants of the receptor components cause similar syndromes with varying severity of intellectual impairment, autism, epilepsy, and motor dysfunction. In Mice with reduced NMDA receptor activity, schizophrenia-like behaviors are revealed.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 1

Entrez Gene IDs

2902 (Human); 14810 (Mouse); 24408 (Rat)

Alternate Names

GluN1; GRIN1; MRD8; NDHMSD; NDHMSR;NMD-R1; NMDA R, NR1 Subunit; NMDA1; NMDAR1; NMD-R1; NR1