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Mouse Endoglin/CD105 Biotinylated Antibody

Catalog #: BAF1320
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF1320
Endoglin/CD105 in Mouse Lung.
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Product Details
Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Endoglin/CD105 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Endoglin/CD105 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human Endoglin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105
Glu27-Gly581
Accession # Q8K100
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Endoglin/CD105 Fc Chimera (Catalog # 1320-EN)
Flow Cytometry
0.25 µg/106 cells
MS-1 mouse cell line
Immunohistochemistry
5-15 µg/mL
See below

Mouse Endoglin/CD105 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Endoglin/CD105 Antibody (Catalog # MAB13201)

Standard: Recombinant Mouse Endoglin/CD105 Fc Chimera Protein, CF (Catalog # 1320-EN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Endoglin/CD105 antibody in Mouse Lung by Immunohistochemistry (IHC-Fr). View Larger

Endoglin/CD105 in Mouse Lung. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse lung using 15 µg/mL Goat Anti-Mouse Endoglin/CD105 Biotinylated Antigen Affinity-purified Poly-clonal Antibody (Catalog # BAF1320) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Flow Cytometry View Larger

Detection of Endoglin/CD105 in MS-1 Mouse Cell Line by Flow Cytometry. MS-1 mouse cell line was stained with Goat Anti-Mouse Endoglin/CD105 Biotinylated Polyclonal Antibody (Catalog # BAF1320, filled histogram) or control antibody (BAF108, open histogram), followed by Phycoerythrin-conjugated Streptavidin (F0040). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

References
  1. Ge, A.Z. and E.C. Butcher (1994) Gene 138:201.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs
2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

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Citations for Mouse Endoglin/CD105 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Decellularized Pancreatic Tail as Matrix for Pancreatic Islet Transplantation into the Greater Omentum in Rats
    Authors: Z Berkova, K Zacharovov, A Patikova, I Leontovyc, Z Hladikova, D Cerveny, E Tihlarikov, V Nedela, P Girman, D Jirak, F Saudek
    Journal of functional biomaterials, 2022-09-30;13(4):.
    Species: Rat
    Sample Types: Whole Cell
    Applications: Flow Cytometry
  2. Targeting Endoglin Expressing Cells in the Tumor Microenvironment Does Not Inhibit Tumor Growth in a Pancreatic Cancer Mouse Model
    Authors: MJA Schoonderw, SK Hakuno, M Sassen, EB Kuhlemaije, M Paauwe, M Slingerlan, MF Fransen, LJAC Hawinkels
    OncoTargets and Therapy, 2021-10-29;14(0):5205-5220.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Reducing Endoglin Activity Limits Calcineurin and TRPC‐6 Expression and Improves Survival in a Mouse Model of Right Ventricular Pressure Overload
    Authors: Navin K Kapur, Xiaoying Qiao, Vikram Paruchuri, Emily E Mackey, Gerard H Daly, Kishan Ughreja et al.
    Journal of the American Heart Association
  4. Long-Term Retinal PEDF Overexpression Prevents Neovascularization in a Murine Adult Model of Retinopathy.
    Authors: Haurigot V, Villacampa P, Ribera A, Bosch A, Ramos D, Ruberte J, Bosch F
    PLoS ONE, 2012-07-20;7(7):e41511.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Reduced Endoglin Activity Limits Cardiac Fibrosis and Improves Survival in Heart Failure
    Authors: Navin K. Kapur, Szuhuei Wilson, Adil A. Yunis, Xiaoying Qiao, Emily Mackey, Vikram Paruchuri et al.
    Circulation
  6. ENDOGLIN/CD105 is expressed in KIT positive cells in the gut and in gastrointestinal stromal tumours
    Authors: Petra Gromova, Brian P. Rubin, An Thys, Pierre Cullus, Christophe Erneux, Jean-Marie Vanderwinden
    Journal of Cellular and Molecular Medicine
  7. Endoglin expression in the endothelium is regulated by Fli-1, Erg, and Elf-1 acting on the promoter and a -8-kb enhancer.
    Authors: Pimanda JE, Chan WY, Donaldson IJ, Bowen M, Green AR, Gottgens B
    Blood, 2006-02-16;107(12):4737-45.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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