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Mouse DSPG3 Antibody

Catalog #: MAB2875 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2875
MAB2875-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DSPG3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DSPG3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human DSPG3 is observed.
Source
Monoclonal Rat IgG2B Clone # 340908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DSPG3
Ala20-Ile322
Accession # P70186
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse DSPG3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DSPG3

DSPG3, also known as proteoglycan-Lb and Epiphycan, is a 95‑100 kDa member of the class III subfamily within the SLRP (small leucine-rich proteoglycan) family. The secreted 303 aa mature segment contains multiple sites for O-linked glycation, and seven leucine-rich repeats, flanked by N- and C-terminal cysteine-rich regions. Human DSPG3 shares 96%, 95%, 85% and 84% aa sequence identity with canine, bovine, rat and mouse DSPG3, respectively. DSPG3 is principally a component of cartilage.

Long Name
Dermatan Sulfate Proteoglycan 3
Entrez Gene IDs
1833 (Human); 13516 (Mouse)
Alternate Names
Dermatan sulfate proteoglycan 3dermatan sulphate proteoglycan 3; DSPG3; DSPG3proteoglycan-lb; Epiphycan; EPYC; PGLB; PG-Lb; Proteoglycan-Lb; SLRR3B; SLRR3Bepiphycan proteoglycan; Small chondroitin/dermatan sulfate proteoglycan

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