Mouse DNER Antibody

Catalog #: MAB2254 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2254
MAB2254-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DNER Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DNER in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DNER is observed.

Source

Monoclonal Rat IgG₁ Clone # 298306

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DNER
Ala26-His637
Accession # Q8JZM4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse DNER under non-reducing conditions only

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of mouse brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNER

DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch.

Long Name

Delta/Notch-like EGF Repeat-containing Transmembrane

Entrez Gene IDs

92737 (Human); 227325 (Mouse)

Alternate Names

bet; delta and Notch-like epidermal growth factor-related receptor; delta/notch-like EGF repeat containing; delta-notch-like EGF repeat-containing transmembrane; DNER; H_NH0150O02.1; UNQ26; WUGSC:H_NH0150O02.1