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Mouse Dectin-2/CLEC6A alpha Isoform Biotinylated Antibody

Catalog #: BAF1525
3 Citations 1 Review Datasheet / COA / SDS
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BAF1525
Product Details
Citations (3)
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Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Dectin-2/CLEC6A alpha Isoform Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Dectin‑2 alpha /CLEC6A in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human DLEC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑2 alpha /CLEC6A
Phe43-Leu209
Accession # Q9JKF4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Dectin‑2/CLEC6A alpha Isoform (Catalog # 1525-DC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-2/CLEC6A

Dectin-2 alpha, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 alpha, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3‑6). The CRD of Dectin-2 alpha contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 alpha selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 alpha induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 alpha is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4+CD25+ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 alpha shares 71% aa sequence identity with human and bovine Dectin-2.

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Kanazawa, N. et al. (2004) Immunobiology 209:179.
  3. Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
  4. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
  5. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
  6. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
  7. McGreal, E.P. et al. (2006) Glycobiology 16:422.
  8. Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
  9. Aragane, Y. et al. (2003) J. Immunol. 171:3801.
Entrez Gene IDs
93978 (Human); 56620 (Mouse)
Alternate Names
CLEC4N; CLEC6A; CLECSF10; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10; C-type lectin domain family 6 member A; C-type lectin domain family 6, member A; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin 2; Dectin2; Dectin-2; Dendritic cell-associated C-type lectin 2

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Citations for Mouse Dectin-2/CLEC6A alpha Isoform Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation
    Authors: Giang T. Nguyen, Shuying Xu, Walter Adams, John M. Leong, Stephen C. Bunnell, Michael K. Mansour et al.
    iScience
  2. The balance between gasdermin D and STING signaling shapes the severity of schistosome immunopathology
    Authors: P Kalantari, I Shecter, J Hopkins, A Pilotta Go, Y Morales, BF Harandi, S Sharma, MJ Stadecker
    Proceedings of the National Academy of Sciences of the United States of America, 2023-03-21;120(13):e2211047120.
  3. CD209a Synergizes with Dectin-2 and Mincle to Drive Severe Th17 Cell-Mediated Schistosome Egg-Induced Immunopathology
    Authors: P Kalantari, Y Morales, EA Miller, LD Jaramillo, HE Ponichtera, MA Wuethrich, C Cheong, MC Seminario, JM Russo, SC Bunnell, MJ Stadecker
    Cell Rep, 2018-01-30;22(5):1288-1300.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Mouse Dectin-2/CLEC6A alpha Isoform Biotinylated Antibody
By Anonymous on 06/28/2016
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Goat