Mouse CXCR5 APC-conjugated Antibody Summary
Met1-Pro57
Accession # Q04683
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CXCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB6198A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
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Detection of CXCR5 in Mouse T Follicular Helper Cells by Flow Cytometry. Mouse T follicular helper cells from immunized Balb/c mice were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse CXCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB6198A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human CXCR5 by Flow Cytometry The percentages of CD4+CXCR5+ICOS5+ T cells in peripheral blood of patients with T1D.Peripheral blood mononuclear cells (PMBCs) from T1D patients (n = 54) and healthy controls (n = 31) were stained with labelled antibodies as described in Methods. A. Representative dot plots of CD4+CXCR5+ICOS5+ T cells in different groups of samples. Values in the upper right quadrant correspond to the percentages of CD4+CXCR5+ICOS5+ T cells. At least about 50,000 events were analyzed for each sample. B. CD4+CXCR5+ICOS5+ T cells were compared between T1D patients and healthy controls. Each data point represents an individual subject. The bars indicate the mean values. Student's unpaired t test was performed. ***, P<0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24278195), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR5
CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7R alpha + precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.
Product Datasheets
Citations for Mouse CXCR5 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Inhibition of a BTK-IDO-mTOR axis promotes differentiation of monocyte-lineage inflammatory dendritic cells and enhances anti-tumor T cell immunity
Authors: Madhav D. Sharma, Rafal Pacholczyk, Huidong Shi, Zuzana J. Berrong, Yousef Zakharia, Austin Greco et al.
Immunity
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Inhibition of increased circulating Tfh cell by anti-CD20 monoclonal antibody in patients with type 1 diabetes.
Authors: Xu, Xinyu, Shi, Yun, Cai, Yun, Zhang, Qingqing, Yang, Fan, Chen, Heng, Gu, Yong, Zhang, Mei, Yu, Liping, Yang, Tao
PLoS ONE, 2013-11-20;8(11):e79858.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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