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Mouse CXCL4/PF4 Biotinylated Antibody

Catalog #: BAF595
1 Citation Datasheet / COA / SDS
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BAF595
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Citations (1)
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Mouse CXCL4/PF4 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL4/PF4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) CXCL4, recombinant mouse (rm) MIG, rmI-TAC, rhIL-8, and rhIP-10 is observed. 
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL4/PF4 (R&D Systems, Catalog # 595-P4)
Val30-Ser105
Accession # Q9Z126
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CXCL4/PF4 (Catalog # 595-P4)

Mouse CXCL4/PF4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CXCL4/PF4 Antibody (Catalog # MAB595)

Standard: Recombinant Mouse CXCL4/PF4 Protein, CF (Catalog # 595-P4)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL4/PF4

CXCL4 is a member of the CXC chemokine family. Mouse CXCL4 is a 105 amino acid (aa) protein with a 29 aa signal sequence and a 76 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Mouse and human CXCL4 share a 64% identity. Mouse and rat CXCL4 share 89% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other fuctions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

References
  1. Poncz, M. et al. (1987) Blood 69:219.
  2. Scheuerer, B. et al. (2000) Blood 95:1158.
  3. Perollet, C. et al. (1998) Blood 91:3289.
  4. Petersen, F. et al. (1998) J. Immunol. 161:4347.
  5. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
  6. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
Entrez Gene IDs
5196 (Human); 56744 (Mouse); 360918 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A

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Citation for Mouse CXCL4/PF4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-21 induces the functional maturation of murine NK cells.
    Authors: Brady J, Hayakawa Y, Smyth MJ, Nutt SL
    J. Immunol., 2004-02-15;172(4):2048-58.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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