Mouse Cryptic Antibody

Catalog #: MAB18401 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB18401
MAB18401-SP

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Mouse Cryptic Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Cryptic in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human Cryptic is observed.

Source

Monoclonal Rat IgG_2B Clone # 221428

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cryptic
Tyr36-Gly177
Accession # P97766

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

D3 mouse embryonic stem cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Cryptic in D3 Mouse Cell Line. Cryptic was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse Cryptic Monoclonal Antibody (Catalog # MAB18401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cryptic

Cryptic, also known as CFC-1, is a member of the Epidermal Growth Factor-Cripto/FRL-1/Cryptic (EGF-CFC) family of signaling proteins. It is expressed during mesoderm differentation and is necessary for determining left-right asymmetry. Cryptic functions as a co-receptor that regulates signaling by some TGF-beta superfamily members.

Entrez Gene IDs

55997 (Human); 12627 (Mouse)

Alternate Names

CFC1; CFC-1; CFC1B; cripto, FRL-1, cryptic family 1; Cryptic family protein 1; cryptic protein; Cryptic; CRYPTICFLJ77897; HTX2MGC133213

Product Datasheets

Product Datasheet

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SDS

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