Mouse Collagen XIII alpha 1 Antibody

Catalog #: AF4627 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4627
AF4627-SP

Detection of Human Collagen XIII alpha 1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Collagen XIII alpha 1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Collagen XIII alpha 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) COL13A1v4 is observed, and less than 1% cross-reactivity with rhCOL4A1, rhCOL25A1, rhCOL3A1, and rhCOL1A1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Collagen XIII alpha 1
Glu107-Gln565
Accession # AAH34164

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Collagen XIII alpha 1 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4627) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collagen XIII a1 at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Collagen XIII alpha 1 in Mouse Embryo. Collagen XIII a1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4627) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen XIII alpha 1

Collagen XIII alpha 1 is an 85-95 kDa protein in the type 2 transmembrane collagen family (1). Mature mouse Collagen XIII alpha 1 consists of a 40 amino acid (aa) cytoplasmic domain, a 19 aa transmembrane segment, and a 692 aa extracellular domain (ECD). The ECD contains three collagenous regions separated by shorter non‑collagenous regions (2, 3). Within comparable regions of the ECD, mouse Collagen XIII alpha 1 shares 85% and 88% aa sequence identity with human and rat Collagen XIII alpha 1, respectively. Mouse Collagen XIII alpha 1 is extensively spliced, with some isoforms showing a tissue specific distribution (2, 4). Collagen XIII alpha 1 is widely expressed during development and in the adult (4, 5). It localizes to intercellular adherens junctions and cell-matrix focal adhesions (6, 7). Collagen XIII alpha 1 assembles into disulfide-linked trimers, a process that is enhanced by proline hydroxylation (2, 8). Trimerization involves triple helix formation within the collagenous domains, although portions of the non‑collagenous regions can also form coiled coils (8‑10). The ECD of trimeric Collagen XIII alpha 1 is an extended rod-like structure with two flexible hinges that correspond to non‑collagenous regions (11). Collagen XIII alpha 1 clusters in cholesterol-rich domains on the plasma membrane (2, 12), and it can be cleaved from the cell surface or intracellularly by a furin-like protease (12). Collagen XIII alpha 1 binds the extracellular matrix molecules fibronectin, heparin, integrin alpha 1, nidogen‑2, and perlecan (11, 13). The shed ECD retains its ability to bind fibronectin and can interfere with matrix formation (14).

References