Mouse Coagulation Factor III/TF DuoSet ELISA
Mouse Coagulation Factor III/TF DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.
Citations for Mouse Coagulation Factor III/TF DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Hutchinson-Gilford progeria syndrome mice display accelerated arterial thrombus formation and increased platelet reactivity
Authors: Puspitasari, YM;Ministrini, S;Han, J;Karch, C;Prisco, F;Liberale, L;Bengs, S;Akhmedov, A;Montecucco, F;Beer, JH;Lüscher, TF;Bongiovanni, D;Camici, GG;
Thrombosis research
Species: Transgenic Mouse
Sample Types: Plasma, Tissue Homogenates
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A TLR4-independent critical role for CD14 in intracellular LPS sensing
Authors: SO Vasudevan, AJ Russo, P Kumari, SK Vanaja, VA Rathinam
Cell Reports, 2022-05-03;39(5):110755.
Species: Mouse
Sample Types: Plasma
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Loss of Sirt3 accelerates arterial thrombosis by increasing formation of neutrophil extracellular traps and plasma tissue factor activity
Authors: DS Gaul, J Weber, LJ Van Tits, S Sluka, L Pasterk, MF Reiner, N Calatayud, C Lohmann, R Klingenber, J Pahla, D Vdovenko, FC Tanner, GG Camici, U Eriksson, J Auwerx, F Mach, S Windecker, N Rodondi, TF L Uuml Sch, S Winnik, CM Matter
Cardiovasc. Res., 2018-07-01;0(0):.
Species: Mouse
Sample Types: Plasma
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