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Mouse CD8 alpha APC-conjugated Antibody

Catalog #: FAB116A
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB116A-100
FAB116A-025
Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD8 alpha APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects both the alpha and alpha ' chains of mouse CD8 (1).
Source
Monoclonal Rat IgG2A Clone # 53-6.7
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse thymus or spleen
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD8a antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD8a APC-conjugated Monoclonal Antibody (Catalog # FAB116A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References
  1. Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. Hayakawa, K. et al. (1994) Science 263:1131.
  4. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  5. Rocha, B. et al. (1992) Immunol. Today 13:449.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. Vermec, D. et al. (1992) J. Exp. Med. 176:47.
  8. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
Entrez Gene IDs
925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names
CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

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Citations for Mouse CD8 alpha APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. PD-L2 Blockade Exacerbates Liver Lesion in Mice Infected with Capillaria hepatica through Reducing Alternatively Activated Macrophages
    Authors: M Huang, X Li, X Zheng, F Wang, Y Zou, L Wang
    Tropical medicine and infectious disease, 2023-01-06;8(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Transgenic Overexpression of Galectin-3 in Pancreatic beta Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33
    Authors: N Jovicic, I Petrovic, N Pejnovic, B Ljujic, M Miletic Ko, S Pavlovic, I Jeftic, A Djukic, I Srejovic, V Jakovljevi, ML Lukic
    Frontiers in Pharmacology, 2021-11-05;12(0):714683.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Ouabain Attenuates Sepsis-Induced Immunosuppression in Mice by Activation and Anti-Apoptosis of T Cells
    Authors: R Niu, H Gao, Y Zhou, J Zhang
    Med. Sci. Monit., 2018-05-02;24(0):2720-2727.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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