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Mouse CD47 N-terminal IgV-like Extracellular Domain Biotinylated Antibody

Catalog #: BAF1866
1 Citation Datasheet / COA / SDS
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BAF1866
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Citations (1)
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Mouse CD47 N-terminal IgV-like Extracellular Domain Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD47 N-terminal IgV-like Extracellular Domain in Western blots. In Western blots, approximately 15% cross‑reactivity with recombinant human CD47 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD47 N-terminal IgV-like Extracellular Domain
Gln19-Pro158
Accession # NP_034711
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD47 N-terminal IgV-like Extracellular Domain
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse ovary and thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD47

CD47, also known as integrin-associated protein, is an integral membrane protein with a single IgV-like domain at its N-terminus followed by five membrane-spanning regions. CD47 is expressed ubiquitously. It is a ligand for the transmembrane signal regulatory protein alpha 1 (SIRP alpha ) and also functions as a receptor for thrombospondin.

Entrez Gene IDs
961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen

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Citation for Mouse CD47 N-terminal IgV-like Extracellular Domain Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CD47 is a negative regulator of intestinal epithelial cell self-renewal following DSS-induced experimental colitis
    Authors: Y He, X Sun, W Rong, R Yang, H Liang, Y Qi, L Li, K Zen
    Sci Rep, 2020-06-23;10(1):10180.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot

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