Mouse CD200R1 Biotinylated Antibody

Catalog #: BAF2554 Datasheet / COA / SDS

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BAF2554

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Mouse CD200R1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD200 R1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human CD200 R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CD200 R1 Fc Chimera (Catalog # 2554-CD)

Flow Cytometry

2.5 µg/10⁶ cells

MC/9‑2 mouse masT cell line

Immunocytochemistry

5-15 µg/mL

Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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CD200R1 in Mouse Splenocytes. CD200R1 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD200R1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2554) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1‑3). The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, (5‑7) while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). The CD200 R-like molecules may interact differently with CD200 (11, 12). The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals (13‑15). CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 16).

References

Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
Wright, G.J. et al. (2003) J. Immunol. 171:3034.
Shiratori, I. et al. (2005) J. Immunol. 175:4441.
Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
Fallarino, F. et al. (2004) J. Immunol. 173:3748.
Wright, G.J. et al. (2001) Immunology 102:173.
Hoek, R.M. et al. (2000) Science 290:1768.
Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
Hatherley, D. et al. (2005) J. Immunol. 175:2469.
Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
Zhang, S. and J.H. Phillips (2006) J. Leukoc. Biol. 79:363.
Zhang, S. et al. (2004) J. Immunol. 173:6786.
Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.

Long Name

CD200 Receptor 1

Entrez Gene IDs

131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)

Alternate Names

CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor