Mouse CD163 Antibody Summary
Val39-Thr1045
Accession # Q2VLH6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse CD163 by Western Blot. Western Blot shows lysates of mouse liver. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB7435) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD163 at approximately 140 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
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Detection of CD163 in Neuro2A cells (Positive). CD163 was detected in fixed Neuro2A cells (Positive) using Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB7435) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD163
CD163, also known as M130 and p155, is a 130-160 kDa type I transmembrane protein belonging to group B of the cysteine-rich scavenger receptor family (1). Mature mouse CD163 consists of a 1007 amino acid (aa) extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (2). Alternative splicing of mouse CD163 generates an isoform with a substitution in the cytoplasmic region. Within the ECD, mouse CD163 shares 75% and 90% aa sequence identity with human and rat CD163, respectively. CD163 is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10 (3-5). A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage (6, 7) in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6 (8-10). CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines (11). It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin (12, 13). It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion (14). CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis (15, 16).
- Kowal, K. et al. (2011) Folia Histochem. Cytobiol. 49:365.
- Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
- Hogger, P. et al. (1998) J. Immunol. 161:1883.
- Sulahian, T.H. et al. (2000) Cytokine 12:1312.
- Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
- Etzerodt, A. et al. (2010) J. Leukoc. Biol. 88:1201.
- Moreno, J.A. et al. (2012) Eur. Heart J. 33:252.
- Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
- Timmermann, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
- Sulahian, T.H. et al. (2004) J. Leukoc. Biol. 76:271.
- Fabriek, B.O. et al. (2009) Blood 113:887.
- Kristiansen, M. et al. (2001) Nature 409:198.
- Schaer, D.J. et al. (2006) Blood 107:373.
- Fabriek, B.O. et al. (2007) Blood 109:5223.
- Bover, L.C. et al. (2007) J. Immunol. 178:8183.
- Moreno, J.A. et al. (2009) Atherosclerosis 207:103.
Product Datasheets
Citation for Mouse CD163 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Preclinical Efficacy of VTX-0811: A Humanized First-in-Class PSGL-1 mAb Targeting TAMs to Suppress Tumor Growth
Authors: Novobrantseva, T;Manfra, D;Ritter, J;Razlog, M;O'Nuallain, B;Zafari, M;Nowakowska, D;Basinski, S;Phennicie, RT;Nguyen, PA;Brehm, MA;Sazinsky, S;Feldman, I;
Cancers
Species: Mouse
Sample Types: Whole Cells
Applications: FACS
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