Home / CCR6 / Mouse CCR6 APC-conjugated Antibody

Mouse CCR6 APC-conjugated Antibody

Catalog #: FAB590A
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB590A-025
FAB590A-100
Detection of CCR6 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR6 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5.
Source
Monoclonal Rat IgG2A Clone # 140706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
Met1-Met367
Accession # O54689
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR6 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse CCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB590A) or (B) Rat IgG2A control antibody (Catalog # IC006A).and Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Entrez Gene IDs
1235 (Human); 12458 (Mouse); 308163 (Rat)
Alternate Names
BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKRL3; CKR-L3; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPRCY4; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22

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Citations for Mouse CCR6 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Essential role for CCR6 in certain inflammatory diseases demonstrated using specific antagonist and knockin mice
    Authors: R Robert, C Ang, G Sun, L Juglair, EX Lim, LJ Mason, NL Payne, CC Bernard, CR Mackay
    JCI Insight, 2017-08-03;2(15):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Respiratory Syncytial virus infection compromises asthma tolerance by recruiting interleukin-17A-producing cells via CCR6-CCL20 signaling
    Authors: T Shi, Y He, W Sun, Y Wu, L Li, Z Jie, X Su
    Mol. Immunol., 2017-06-07;88(0):45-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis.
    Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S
    J Invest Dermatol, 2012-08-16;133(1):164-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation
    Authors: J Emgård, H Kammoun, B García-Cas, J Chesné, SM Parigi, JM Jacob, HW Cheng, E Evren, S Das, P Czarnewski, N Sleiers, F Melo-Gonza, E Kvedaraite, M Svensson, E Scandella, MR Hepworth, S Huber, B Ludewig, L Peduto, EJ Villablanc, H Veiga-Fern, JP Pereira, RA Flavell, T Willinger
    Immunity, 2018-01-16;48(1):120-132.e8.

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