Home / CCR3 / Mouse CCR3 Fluorescein-conjugated Antibody

Mouse CCR3 Fluorescein-conjugated Antibody

Catalog #: FAB729F
27 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB729F-025
FAB729F-100
Detection of CCR3 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (27)
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Mouse CCR3 Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR3. Specifically stains mouse CCR3 transfectants but not the parental cell lines. Does not cross‑react with Y3 cells transfected with human CCR3.
Source
Monoclonal Rat IgG2A Clone # 83101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse CCR3
Met1-Phe359
Accession # P51678
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR3 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB729F) and Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A). Quadrant markers were set based on control antibody staining (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Mouse CCR3 by Flow Cytometry View Larger

Detection of Mouse CCR3 by Flow Cytometry Gal-3 deficient Eos exhibit decreased migration toward eotaxin-1. (A) Migration of WT and Gal-3-/- BM-derived Eos toward murine eotaxin-1 (100 nM) in vitro using 96-well Transwell ® Chambers. Results are represented as percent cell migration relative to WT Eos. (B) Expression of CCR3 by WT and Gal-3-/- Eos by flow cytometry using FITC-conjugated rat anti-mouse CCR3 with FITC-conjugated rat IgG2a as isotype control. Representative data of two independent experiments with Eos from different mice is shown. (C) Migration of WT Eos suspended in medium alone or medium containing lactose or maltose toward murine eotaxin-1. Number of cells that migrated in each case was determined and expressed as the average number of cells/field. Combined data (mean ± SEM) from three independent experiments in duplicate is shown in (A) and (C). #p <0.05 versus WT Eos in (A). Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fphar.2013.00037/abstract), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

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Citations for Mouse CCR3 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Nematode-Infected Mice Acquire Resistance to Subsequent Infection With Unrelated Nematode by Inducing Highly Responsive Group 2 Innate Lymphoid Cells in the Lung
    Authors: Koubun Yasuda, Takumi Adachi, Atsuhide Koida, Kenji Nakanishi
    Frontiers in Immunology
  2. Sweet taste receptor agonists attenuate macrophage IL-1 beta expression and eosinophilic inflammation linked to autophagy deficiency in myeloid cells
    Authors: Lee J, Kim SJ, Choi GE et al.
    Clinical and translational medicine
  3. Th2 cells and macrophages cooperatively induce allergic inflammation through histamine signaling
    Authors: N Iwasaki, S Terawaki, K Shimizu, D Oikawa, H Sakamoto, K Sunami, F Tokunaga
    PLoS ONE, 2021-03-04;16(3):e0248158.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  4. Regulation of Eosinophil Recruitment and Allergic Airway Inflammation by Tropomyosin Receptor Kinase A
    Authors: M Dileepan, XN Ge, I Bastan, YG Greenberg, Y Liang, P Sriramarao, SP Rao
    J. Immunol., 2019-12-23;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Inhibition of soluble epoxide hydrolase attenuates eosinophil recruitment and food allergen-induced gastrointestinal inflammation
    Authors: I Bastan, XN Ge, M Dileepan, YG Greenberg, AG Guedes, SH Hwang, BD Hammock, RJ Washabau, SP Rao, P Sriramarao
    J. Leukoc. Biol., 2018-01-17;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production
    Authors: P Rozenberg, H Reichman, I Zab-Bar, M Itan, M Pasmanik-C, C Bouffi, U Qimron, I Bachelet, PC Fulkerson, ME Rothenberg, A Munitz
    Sci Rep, 2017-07-19;7(1):5922.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Frontline Science: Eosinophil-deficient MBP-1 and EPX double-knockout mice link pulmonary remodeling and airways dysfunction with type 2 inflammation
    Authors: SI Ochkur, AD Doyle, EA Jacobsen, WE LeSuer, W Li, CA Protheroe, KR Zellner, D Colbert, HH Shen, CG Irvin, JJ Lee, NA Lee
    J. Leukoc. Biol., 2017-05-17;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Single-cell analysis of mixed-lineage states leading to a binary cell fate choice.
    Authors: Olsson A, Venkatasubramanian M, Chaudhri V, Aronow B, Salomonis N, Singh H, Grimes H
    Nature, 2016-08-31;537(7622):698-702.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1
    Proc Natl Acad Sci USA, 2016-07-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Generation of eosinophils from cryopreserved murine bone marrow cells.
    Authors: Schollaert, Kaila L, Stephens, Michael, Gray, Jerilyn, Fulkerson, Patricia
    PLoS ONE, 2014-12-31;9(12):e116141.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge.
    Authors: Takeda K, Shiraishi Y, Ashino S, Han J, Jia Y, Wang M, Lee N, Lee J, Gelfand E
    J Allergy Clin Immunol, 2014-10-11;135(2):451-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  12. Bone Marrow Derived Eosinophil Cultures.
    Authors: Lu T, Rothenberg M
    Bio Protoc, 2014-06-20;4(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils.
    Authors: Doyle, Alfred D, Jacobsen, Elizabet, Ochkur, Sergei I, Willetts, Lian, Shim, Kelly, Neely, Joseph, Kloeber, Jake, Lesuer, Will E, Pero, Ralph S, Lacy, Paige, Moqbel, Redwan, Lee, Nancy A, Lee, James J
    J Leukoc Biol, 2013-04-29;94(1):17-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Eosinophil-expressed galectin-3 regulates cell trafficking and migration.
    Authors: Ge X, Ha S, Liu F, Rao S, Sriramarao P
    Front Pharmacol, 2013-04-05;4(0):37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Targeted ablation of miR-21 decreases murine eosinophil progenitor cell growth.
    Authors: Lu, Thomas X, Lim, Eun-Jin, Itskovich, Svetlana, Besse, John A, Plassard, Andrew J, Mingler, Melissa, Rothenberg, Joelle A, Fulkerson, Patricia, Aronow, Bruce J, Rothenberg, Marc E
    PLoS ONE, 2013-03-22;8(3):e59397.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Flow Cytometry
  16. N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation.
    Authors: Bahaie NS, Kang BN, Frenzel EM, Hosseinkhani MR, Ge XN, Greenberg Y, Ha SG, Demetriou M, Rao SP, Sriramarao P
    J. Biol. Chem., 2011-09-12;286(44):38231-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. Myeloid-Derived Suppressor Cells Infiltrate the Heart in Acute Trypanosoma cruzi Infection.
    Authors: Cuervo H, Guerrero NA, Carbajosa S, Beschin A, De Baetselier P, Girones N, Fresno M
    J. Immunol., 2011-07-29;187(5):2656-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. SIRP{alpha}/CD172a Regulates Eosinophil Homeostasis.
    Authors: Verjan Garcia N, Umemoto E, Saito Y, Yamasaki M, Hata E, Matozaki T, Murakami M, Jung YJ, Woo SY, Seoh JY, Jang MH, Aozasa K, Miyasaka M
    J. Immunol., 2011-07-20;187(5):2268-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism.
    Authors: Cain DW, Snowden PB, Sempowski GD, Kelsoe G
    PLoS ONE, 2011-05-31;6(5):e19957.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice.
    Authors: Yamada T, Tani Y, Nakanishi H
    FASEB J., 2010-10-19;25(2):561-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. CD28 and ICOS play complementary non-overlapping roles in the development of Th2 immunity in vivo.
    Authors: Shilling RA, Clay BS, Tesciuba AG, Berry EL, Lu T, Moore TV, Bandukwala HS, Tong J, Weinstock JV, Flavell RA, Horan T, Yoshinaga SK, Welcher AA, Cannon JL, Sperling AI
    Cell. Immunol., 2009-07-10;259(2):177-84.
    Species: Mouse
    Sample Types: BALF
    Applications: Flow Cytometry
  22. CD43 regulates Th2 differentiation and inflammation.
    Authors: Cannon JL, Collins A, Mody PD, Balachandran D, Henriksen KJ, Smith CE, Tong J, Clay BS, Miller SD, Sperling AI
    J. Immunol., 2008-06-01;180(11):7385-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Intravascular inactivation of CCR5 by n-Nonanoyl-CC chemokine ligand 14 and inhibition of allergic airway inflammation.
    Authors: Gupta S, Fuchs B, Schulz-Maronde S, Heitland A, Escher SE, Mack M, Tillmann HC, Braun A, Forssmann WG, Elsner J, Forssmann U
    J. Leukoc. Biol., 2007-12-06;83(3):765-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Regulation of allergen-induced bone marrow eosinophilopoiesis: role of CD4+ and CD8+ T cells.
    Authors: Radinger M, Bossios A, Alm AS, Jeurink P, Lu Y, Malmhall C, Sjostrand M, Lotvall J
    Allergy, 2007-12-01;62(12):1410-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma.
    Authors: Ochkur SI, Jacobsen EA, Protheroe CA, Biechele TL, Pero RS, McGarry MP, Wang H, O&amp;apos;Neill KR, O'Neill KR, Colbert DC, Colby TV, Shen H, Blackburn MR, Irvin CC, Lee JJ, Lee NA
    J. Immunol., 2007-06-15;178(12):7879-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation.
    Authors: Fulkerson PC, Fischetti CA, McBride ML, Hassman LM, Hogan SP, Rothenberg ME
    Proc. Natl. Acad. Sci. U.S.A., 2006-10-23;103(44):16418-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. CD48 is an allergen and IL-3-induced activation molecule on eosinophils.
    Authors: Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F
    J. Immunol., 2006-07-01;177(1):77-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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