Mouse BTN1A1/Butyrophilin Biotinylated Antibody

Catalog #: BAF4765 Datasheet / COA / SDS

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BAF4765

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Mouse BTN1A1/Butyrophilin Biotinylated Antibody Summary

Specificity

Detects mouse BTN1A1/Butyrophilin in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BTN1A1/Butyrophilin
Ala27-Trp247
Accession # Q62556

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse BTN1A1/Butyrophilin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN1A1/Butyrophilin

Butyrophilin (Greek for “being like butter”; also Btn1a1) is a 66 kDa member of the BTN/MOG family of proteins. It is expressed on secretory mammary epithelium and associates with xanthine dehydrogenase. This is necessary for milk-lipid droplet secretion. Mature mouse Butyrophilin is 498 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 221 aa extracellular domain (ECD) (aa 27‑247) and a 256 aa cytoplasmic region. The ECD contains two V-type Ig‑like domains (aa 29‑141 and aa 149‑235). There are two potential splice variants. One shows a deletion of aa 145‑238, while a second shows a 36 aa substitution for aa 144‑524. Over aa 27‑247, mouse Butyrophilin shares 85% and 67% aa identity with rat and human Butyrophilin, respectively.

Entrez Gene IDs

696 (Human); 12231 (Mouse); 306956 (Rat)

Alternate Names

BTBTNbK14H9.2 (butyrophilin, subfamily 1, member A1); BTN; BTN1A1; butyrophilin subfamily 1 member A1; butyrophilin, subfamily 1, member A1