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Mouse ADAM10 Ectodomain Antibody

Catalog #: MAB946
15 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB946-SP
MAB946-500
MAB946-100
Detection of Mouse ADAM10 by Western Blot
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ADAM10 Ectodomain Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed.
Source
Monoclonal Rat IgG2A Clone # 139712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
Thr215-Glu673
Accession # O35598
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse ADAM10 (Catalog # 946-AD) under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse ADAM10 by Western Blot View Larger

Detection of Mouse ADAM10 by Western Blot Cre mRNA is present in the blood plasma of Vav-iCre mice and contained in EVs including exosomes.(A) Cre mRNA can be detected in vesicle preparations enriched for exosomes from the blood plasma of Vav-iCre mice by RT-PCR. Each lane represents a result from an individual animal. For detection of Cre mRNA, nested primer PCR was used. The PCR product at 100 bp represents the signal after the second round of amplification. (B) Cre mRNA is localized in the pellet but not in the supernatant after ultracentrifugation of conditioned medium from primary Vav-iCre–positive hematopoietic cells after stimulation by LPS in vitro. Cre mRNA was resistant to RNaseA treatment in all experiments. (C) After treatment with Triton-X to lyse EVs in combination with RNaseA digestion, Cre mRNA is no longer detectable in contrast to RNaseA treatment alone. (D) Vesicular structures between 50 and 100 nm in size were visualized in electron micrographs from Vav-iCre hematopoietic-cell-derived vesicle preparations (scale bar, 50 nm). (E and F) Secreted membrane vesicle subspecies can be separated by density by sucrose gradient ultracentrifugation. Exosomal identity was confirmed by blotting against the specific protein markers ADAM10 and CD9 for all subfractions. (G) Cre protein could not be detected in any of the fractions. Positive controls for all antibodies are shown in boxes to the right. (H) Cre mRNA is present in the exosomal fractions 2–7. The nonexosomal vesicles fractions or apoptotic bodies are characterized by their variability of positive subfractions to complete absence of Cre mRNA. In this experiment, subfractions 9 and 10 are positive, whereas 8, 11, and 12 do not contain any Cre RNA. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24893313), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM10

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

References
  1. Rooke, J. et al. (1996) Science 273:1227.
  2. Pan, D. and Rubin, G.M. (1997) Cell 90:271.
  3. Qi, H. et al. (1999) Science 283:91.
  4. Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
  5. Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
  6. Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
  7. Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
  8. Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
  9. Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Long Name
A Disintegrin and Metalloprotease-like Domain 10
Entrez Gene IDs
102 (Human); 11487 (Mouse)
Alternate Names
a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease

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Citations for Mouse ADAM10 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth
    J Exp Med, 2016-08-08;0(0):.
  2. Distinct T cell receptor signaling pathways drive proliferation and cytokine production in T cells
    Authors: Clifford S. Guy, Kate M. Vignali, Jamshid Temirov, Matthew L Bettini, Abigail E. Overacre, Matthew Smeltzer et al.
    Nature Immunology
  3. ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling
    Authors: N McMillan, GW Kirschen, S Desai, E Xia, SE Tsirka, A Aguirre
    Neural regeneration research, 2022-11-01;17(11):2472-2483.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. CXCR6+CD4+ T cells promote mortality during Trypanosoma brucei infection
    Authors: G Liu, O Abas, AB Strickland, Y Chen, M Shi
    PloS Pathogens, 2021-10-06;17(10):e1009968.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Macroautophagy in Dendritic Cells Controls the Homeostasis and Stability of Regulatory T Cells
    Authors: J Niven, N Madelon, N Page, A Caruso, G Harlé, S Lemeille, CA Seemayer, S Hugues, M Gannagé
    Cell Rep, 2019-07-02;28(1):21-29.e6.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Tetraspanin 3: A central endocytic membrane component regulating the expression of ADAM10, presenilin and the amyloid precursor protein
    Authors: Paul Saftig
    Biochim. Biophys. Acta, 2016-11-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The sheddase ADAM10 is a potent modulator of prion disease.
    Authors: Altmeppen, Hermann, Prox, Johannes, Krasemann, Susanne, Puig, Berta, Kruszewski, Katharin, Dohler, Frank, Bernreuther, Christia, Hoxha, Ana, Linsenmeier, Luise, Sikorska, Beata, Liberski, Pawel P, Bartsch, Udo, Saftig, Paul, Glatzel, Markus
    Elife, 2015-02-05;4(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  8. Pyroptosis of resident macrophages differentially orchestrates inflammatory responses to Staphylococcus aureus in resistant and susceptible mice.
    Authors: Accarias S, Lugo-Villarino G, Foucras G, Neyrolles O, Boullier S, Tabouret G
    Eur J Immunol, 2015-01-21;45(3):794-806.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Extracellular vesicle-mediated transfer of genetic information between the hematopoietic system and the brain in response to inflammation.
    Authors: Ridder K, Keller S, Dams M, Rupp A, Schlaudraff J, Turco D, Starmann J, Macas J, Karpova D, Devraj K, Depboylu C, Landfried B, Arnold B, Plate K, Hoglinger G, Sultmann H, Altevogt P, Momma S
    PLoS Biol, 2014-06-03;12(6):e1001874.
    Species: Mouse
    Sample Types: Extracellular Vesicles
    Applications: Western Blot
  10. Adrenergic regulation of IgE involves modulation of CD23 and ADAM10 expression on exosomes.
    Authors: Padro C, Shawler T, Gormley M, Sanders V
    J Immunol, 2013-10-18;191(11):5383-97.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Deletion of Adam10 in endothelial cells leads to defects in organ-specific vascular structures.
    Authors: Glomski K, Monette S, Manova K, de Strooper B, Saftig P, Blobel CP
    Blood, 2011-06-07;118(4):1163-74.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. A disintegrin and metalloproteinase 10-mediated cleavage and shedding regulates the cell surface expression of CXC chemokine ligand 16.
    Authors: Gough PJ, Garton KJ, Wille PT, Rychlewski M, Dempsey PJ, Raines EW
    J. Immunol., 2004-03-15;172(6):3678-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Adam10-dependent Notch signaling establishes dental epithelial cell boundaries required for enamel formation
    Authors: Thimios A. Mitsiadis, Lucia Jimenez-Rojo, Anamaria Balic, Silvio Weber, Paul Saftig, Pierfrancesco Pagella
    iScience
  14. MicroRNA-138 Overexpression Alters A beta 42 Levels and Behavior in Wildtype Mice
    Authors: Emmanuelle Boscher, Claudia Goupil, Serena Petry, Rémi Keraudren, Andréanne Loiselle, Emmanuel Planel et al.
    Frontiers in Neuroscience
  15. The metalloproteinase ADAM10 requires its activity to sustain surface expression
    Authors: Anke Seifert, Stefan Düsterhöft, Justyna Wozniak, Chek Z. Koo, Michael G. Tomlinson, Elisa Nuti et al.
    Cellular and Molecular Life Sciences

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Mouse ADAM10 Ectodomain Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: 4T1.2 mouse mammary carcinoma cells Species: Mouse

Buffer: TBST
Dilution: 1/200