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Monastrol

Catalog #: 1305
19 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1305/10
1305/50
Monastrol | CAS No. 254753-54-3 | Kinesin Inhibitors
1 Image
Description: Selective inhibitor of mitotic kinesin Eg5

Chemical Name: 1,2,3,4-Tetrahydro-4-(3-hydroxyphenyl)-6-methyl-2-thioxo-5-pyrimidinecarboxylic acid, ethyl ester

Purity: ≥98%

Product Details
Citations (19)
Reviews
Biological Activity Technical Data Additional Information Background Product Datasheets Calculators

Biological Activity

Monastrol is a potent, cell-permeable, small molecule mitosis inhibitor that does not interact with tubulin. Arrests cells in mitosis and specifically inhibits the motility of the mitotic kinesin Eg5, a motor protein required for mitotic spindle formation and maintenance (IC50 = 14 μM).

Technical Data

M.Wt:
292.35
Formula:
C14H16N2O3S
Solubility:
Soluble to 20 mM in ethanol and to 100 mM in DMSO
Purity:
≥98%
Storage:
Store at RT
CAS No:
254753-54-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold with the permission of Harvard University

Background References

  1. High-throughput genotoxicity assay identifies antioxidants as inducers of DNA damage response and cell death.
    Fox JT, Sakamuru S, Huang R
    Proc. Natl. Acad. Sci. U.S.A., 2012;109(14):5423-8.
  2. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
    S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
    Cell Death Dis, 2017;8(7):e2955.
  3. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen.
    Mayer et al.
    Science, 1999;286:971
  4. Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5.
    Kapoor et al.
    J.Cell Biol., 2000;150:975

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Citations for Monastrol

The citations listed below are publications that use Tocris products. Selected citations for Monastrol include:

19 Citations: Showing 1 - 10

  1. Lipid accumulation facilitates mitotic slippage-induced adaptation to anti-mitotic drug treatment.
    Authors: Wong Et al.
    Cell Death Discov  2018;4:109
  2. Chromosome Segregation Is Biased by Kinetochore Size.
    Authors: Drpic Et al.
    Curr Biol  2018;28:1344
  3. Identification of small molecule inhibitors of the Aurora-A/TPX2 complex.
    Authors: Asteriti Et al.
    Oncotarget  2017;8:32117
  4. Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.
    Authors: Shrestha
    Nat Commun  2017;8(1):150
  5. C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle.
    Authors: Floriot Et al.
    Cell Cycle  2015;6:6894
  6. A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly.
    Authors: Barr and Bakal
    Nat Commun  2015;5:10564
  7. Mitotic slippage and expression of survivin are linked to differential sensitivity of human cancer cell-lines to the Kinesin-5 inhibitor monastrol.
    Authors: Asraf Et al.
    PLoS One  2015;10:e0129255
  8. Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation.
    Authors: Kabeche and Compton
    J Biol Chem  2013;502:110
  9. p31(comet) inactivates the chemically induced Mad2-dependent spindle assembly checkpoint and leads to resistance to anti-mitotic drugs.
    Authors: Habu and Matsumoto
    Nature  2013;2:562
  10. Nonautonomous movement of chromosomes in mitosis.
    Authors: Vladimirou Et al.
    Dev Cell  2013;27:60
  11. Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.
    Authors: Shrestha and Draviam
    Curr Biol  2013;23:1514
  12. Automated tracking of mitotic spindle pole positions shows that LGN is required for spindle rotation but not orientation maintenance.
    Authors: Corrigan Et al.
    Springerplus  2013;12:2643
  13. High mitotic activity of Polo-like kinase 1 is required for chromosome segregation and genomic integrity in human epithelial cells.
    Authors: Lera and Burkard
    J Biol Chem  2012;287:42812
  14. Kinesin molecular motor Eg5 functions during polypeptide synthesis.
    Authors: Bartoli Et al.
    Mol Biol Cell  2011;22:3420
  15. Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors.
    Authors: Bassett Et al.
    Sci Rep  2010;190:177
  16. Human condensin function is essential for centromeric chromatin assembly and proper sister kinetochore orientation.
    Authors: Samoshkin Et al.
    PLoS One  2009;4:e6831
  17. Potentiation of kinesin spindle protein inhibitor-induced cell death by modulation of mitochondrial and death receptor apoptotic pathways.
    Authors: Vijapurkar Et al.
    Cancer Res  2007;67:237
  18. Induction of apoptosis by monastrol, an inhibitor of the mitotic kinesin Eg5, is independent of the spindle checkpoint.
    Authors: Chin
    Mol Cancer Ther  2006;5:2580
  19. Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle.
    Authors: Trinkle-Mulcahy Et al.
    Mol Biol Cell  2003;14:107

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