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Janelia Fluor® 669, Maleimide

Catalog #: 8097 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8097/200U
Janelia Fluor® 669, Maleimide | Fluorescent Dyes
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Description: Red dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging

Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-2-oxoethyl)thio)-3,5,6-trifluorobenzoate

Purity: ≥95%

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Biological Activity Technical Data Additional Information Product Datasheets Calculators

Biological Activity

Key information: Janelia Fluor® 669, Maleimide is a red fluorogenic fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 669 nm and 689 nm, respectively.

Please see the product protocol for further information and a guide to protein/antibody labeling.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Optical Data for Janelia Fluor® 669, Maleimide

Emission Color Red
λabs 669 nm
λem 689 nm
Reactive Group Maleimide
Reactivity Thiols
Cell Permeable Yes
Application Confocal Microscopy, dSTORM, STED, Flow Cytometry, Live-cell Imaging

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
718.82
Formula:
C36H33F3N4O5SSi
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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Reconstitution Calculator

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