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Janelia Fluor® 630b, NHS ester

Catalog #: 8155 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8155/200U
Janelia Fluor® 630b, NHS ester | Amine-Reactive Janelia Fluor Dyes
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Description: Blinking fluorescent dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM) including SMLM

Chemical Name: 2,5-Dioxopyrrolidin-1-yl 5,5-dimethyl-3,7-bis(3-(methylsulfonyl)azetidin-1-yl)-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxylate

Purity: ≥90%

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Biological Activity Technical Data Additional Information Product Datasheets Calculators

Biological Activity

Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.

Key Information: Janelia Fluor® 630b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for characterizing the physical properties of dense biomolecule condensates and complex macromolecular assemblies.

Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).

Properties and Photophysical Data: excitation and emission maxima (λ) are 654 nm and 671 nm, respectively.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
735.9
Formula:
C35H37N3O9S2Si
Purity:
≥90%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

Reconstitution Calculator
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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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