Janelia Fluor® 585, NHS ester
Chemical Name: 1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-7-(3,3-difluoro-1-azetidinyl)-9,9-dimethyl-2(9H)-anthracenylidene]-3,3-difluoroazetidinium, inner salt
Purity: ≥95%
Biological Activity
Key Information: Janelia Fluor® 585, NHS ester is an orange fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.Applications: Suitable for confocal microscopy, two-photon excitation fluorescence microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Cell permeable.
Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 585 nm and 609 nm, respectively; quantum yield = 0.78; extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.099; lactone-zwitterion equilibrium constant (KL-Z) < 0.0001. Exhibits low non-specific background staining.
Please see the protocol for further information on protein/antibody labeling and conjugation.
To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was Janelia Fluor® 585, SE.
Optical Data for Janelia Fluor® 585, NHS ester
| Emission Color | Orange |
|---|---|
| Brightness | |
| λabs | 585 nm |
| λem | 609 nm |
| Extinction Coefficient (ε) | 156,000 M-1cm-1 |
| Quantum Yield (φ) | 0.78 |
| Closest Laser line | 561 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.099 |
| Application | Confocal Microscopy, SRM, Two-photon Excitation Fluorescence Microscopy |
| Alternative For | Alexa Fluor® 568, Atto Rho12, Atto Rho101, CF594 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Background References
-
A general method to fine-tune fluorophores for live-cell and in vivo imaging.
Grimm et al.
Nat.Methods, 2017;14:987 -
Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging.
Zheng et al.
ACS Cent.Sci., 2019;5:1602
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