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Janelia Fluor® 549, Tetrazine

Catalog #: 6502
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6502/200U
Janelia Fluor® 549, Tetrazine | Click-Reactive Janelia Fluor Dyes
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Description: Yellow dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging

Chemical Name: 3,6-Di-1-azetidinyl-9-[[4-[(1,2,4,5-tetrazin-3-yl)benzyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt

Product Details
Citations (2)
Reviews
Biological Activity Technical Data Additional Information Product Datasheets Calculators

Biological Activity

Key Information: Janelia Fluor® 549, Tetrazine is a yellow fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live-cell imaging.

Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Janelia Fluor® 549, tetrazine is cell permeable. It can be coupled directly to a protein of interest using bioorthogonal chemistry and unnatural amino acid technology and has been used in this way to label living primary neurons.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Optical Data for Janelia Fluor® 549, Tetrazine

Janelia Fluor<sup>®</sup> 549, Tetrazine Dye Spectra
Emission Color Yellow
Brightness
λabs 549 nm
λem 571 nm
Extinction Coefficient (ε) 101,000 M-1cm-1
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Reactive Group Tetrazine
Reactivity Copper-free click
Cell Permeable Yes
Correction Factor 280 0.169
Application Confocal Microscopy, dSTORM, STED, Live-cell Imaging
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data

M.Wt:
623.66
Formula:
C36H29N7O4
Solubility:
Soluble to 10 mM in DMSO
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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Citations for Janelia Fluor® 549, Tetrazine

The citations listed below are publications that use Tocris products. Selected citations for Janelia Fluor® 549, Tetrazine include:

2 Citations: Showing 1 - 2

  1. Visualizing the disordered nuclear transport machinery in situ.
    Authors: Yu Et al.
    Nature  2023;617:162
  2. Minimal genetically encoded tags for fluorescent protein labeling in living neurons.
    Authors: Arsic Et al.
    Nat.Commun.  2022;13

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