Human XL Cytokine Luminex® Performance Assay 46-plex Fixed Panel

Catalog #: LKTM014B
3 Citations Datasheet / COA / SDS

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LKTM014B

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Human XL Cytokine Luminex® Performance Assay 46-plex Fixed Panel Summary

Assay Type	bead-based multiplex assay for the Luminex^® platform
Format	1 x 96-well microplate and antibody-coated beads
Analytes Detected	Please see analyte list in assay customization tool below.
Performance Validation	Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

Simultaneously profile up to 50 analytes of your choice in one sample
Choose from a wide menu of analytes, including many unique analytes
Requires a small sample volume (<50 µL)
Run your assay in just 3-4 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Please allow additional time for international orders.

Kit Components

Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Standard/Sample Diluent
Wash Buffer
Streptavidin-PE
Foil Plate Sealers (4)
Mixing Bottle
Certificate of Analysis**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Precision

	n=40 (20x2 levels)	n=30
Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 30 different serum assays.
Analyte	Intra-Assay (%CV)	Inter-Assay (%CV)
CD40 Ligand	3.8	10.8
EGF	4.1	8.0
Eotaxin	4.8	7.4
FGF basic	2.0	7.6
Flt-3 Ligand	2.4	8.4
G-CSF	1.8	7.0
GM-CSF	3.4	11.2
Granzyme B	2.9	8.2
GRO alpha	1.9	5.6
GRO beta	1.9	6.8
IFN-alpha 2	3.6	10.0
IFN-beta	2.4	7.1
IFN-gamma	2.3	10.9
IL-1 alpha	5.0	10.6
IL-1 beta	2.8	10.1
IL-1ra	3.6	12.4
IL-2	3.9	9.6
IL-3	3.6	9.9
IL-4	2.4	8.4
IL-5	1.8	8.3
IL-6	2.2	8.8
IL-7	2.8	9.6
IL-8	3.2	84
IL-9	6.2	10.3
IL-10	3.6	11.2
IL-12 p70	2.6	9.8
IL-13	2.5	8.3
IL-15	3.2	11.2
IL-17A	2.5	9.8
IL-17E	3.1	8.1
IL-33	2.9	9.7
IP-10	3.3	6.6
MCP-1	1.8	6.3
MIP-1 alpha	2.9	5.7
MIP-1 beta	1.7	7.9
MIP-3 alpha	3.0	9.4
MIP-3 beta	2.5	8.6
PDGF-AA	2.7	7.1
PDGF-AB/BB	2.2	7.4
PD-L1/B7-H1	2.7	8.3
RANTES	1.8	7.5
TGF-alpha	4.4	9.2
TNF-alpha	3.0	10.3
TNF-beta	2.2	8.0
TRAIL	2.4	7.9
VEGF	2.9	10.1

Accuracy

	Linearity		Spiked Recovery
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
Analyte	N	Average %	N	Average %
*Natural linearity HE = Spiked recovery was not observed due to natural linearity in high endogenous samples
CD40 Ligand	4	131	4	75
EGF*	4	150	HE
Eotaxin	4	68	4	156
FGF basic	4	128	4	87
Flt-3 Ligand	4	119	4	78
G-CSF	4	208	4	34
GM-CSF	4	101	4	98
Granzyme B	4	107	4	96
GRO alpha	4	97	4	139
GRO beta*	4	88	HE
IFN-alpha 2	4	106	4	87
IFN-beta	4	137	4	63
IFN-gamma	4	127	4	73
IL-1 alpha	4	143	3	74
IL-1 beta	4	106	4	94
IL-1ra*	4	126	HE
IL-2	4	115	4	88
IL-3	4	123	4	78
IL-4	4	141	4	58
IL-5	4	113	4	88
IL-6	4	126	4	78
IL-7	4	110	4	90
IL-8	4	118	4	78
IL-9	4	111	4	96
IL-10	4	117	4	90
IL-12 p70	4	111	4	87
IL-13	4	118	4	89
IL-15	4	103	4	76
IL-17A	4	128	4	72
IL-17E	4	150	4	55
IL-33	4	103	4	87
IP-10	4	124	4	72
MCP-1*	4	80	HE
MIP-1 alpha	4	95	4	111
MIP-1 beta	4	109	4	95
MIP-3 alpha	4	115	4	99
MIP-3 beta	4	123	4	76
PDGF-AA*	4	91	HE
PDGF-AB/BB*	4	108	HE
PD-L1/B7-H1	4	106	4	96
RANTES*	4	106	HE
TGF-alpha	4	142	4	80
TNF-alpha	4	134	4	71
TNF-beta	4	131	4	65
TRAIL	4	144	4	69
VEGF*	4	129	HE

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

Scientific Data

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Human XL Cytokine Luminex Panel Standard Curves

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Human XL Cytokine Performance Luminex Panel Serum Sample Values Serum samples from 20 apparently healthy individuals were run on the Human XL Cytokine Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.

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Citations for Human XL Cytokine Luminex® Performance Assay 46-plex Fixed Panel

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Expression pattern of serum interleukin-7 in elderly septic patients and its prognostic value for predicting short-term mortality
Authors: Zhao, J;Zhang, Y;Wang, JY;Wei, B;Liu, YG;
Cytokine
Species: Human
Sample Types: Serum
Adipose Tissue-Derived Products May Present Inflammatory Properties That Affect Chondrocytes and Synoviocytes from Patients with Knee Osteoarthritis
Authors: Cavallo, C;Boffa, A;Salerno, M;Merli, G;Grigolo, B;Filardo, G;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cell
BCA101 is a Tumor-Targeted Bifunctional Fusion Antibody that Simultaneously Inhibits EGFR and TGF-beta Signalling to Durably Suppress Tumor Growth
Authors: SR Boreddy, R Nair, PK Pandey, A Kuriakose, SB Marigowda, C Dey, A Banerjee, H Kulkarni, M Sagar, SR Krishn, S Rao, M A R, V Tiwari, B Alke, PK Mv, M Shri, C Dhamne, S Patel, P Sharma, S Periyasamy, J Bhatnagar, MA Kuriakose, RB Reddy, A Suresh, S Sreenivas, N Govindappa, PR Moole, U Bughani, SL Tan, P Nair
Cancer Research, 2023-06-02;0(0):.
Species: Human
Sample Types: Cell Culture Supernates