Human XL Cytokine Luminex® Kit Performance Assay
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Human XL Cytokine Luminex® Kit Performance Assay Summary
| Panel | Luminex Performance Human XL Cytokine Panel |
| Assay Type | Magnetic bead-based multiplex assay for the Luminex® platform |
| Format | 1 x 96-well microplate and Magnetic antibody-coated beads |
| Species | Human |
| Analytes Detected | Please see analyte list in assay customization tool below. |
| Performance Validation | Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet. |
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Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile up to analytes of your choice in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Premixed cocktail of antibody-coated Magnetic beads
- Premixed cocktail of biotinylated detection antibody
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
- High and low controls with Control Mean Value Card
**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.
Precision
|
Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 30 different serum assays. |
||
| n=40 (20x2 levels) | n=30 | |
|---|---|---|
| Analyte | Intra-Assay (%CV) | Inter-Assay (%CV) |
| CD40 Ligand | 3.8 | 10.8 |
| EGF | 4.1 | 8.0 |
| Eotaxin | 4.8 | 7.4 |
| FGF basic | 2.0 | 7.6 |
| Flt-3 Ligand | 2.4 | 8.4 |
| G-CSF | 1.8 | 7.0 |
| GM-CSF | 3.4 | 11.2 |
| Granzyme B | 2.9 | 8.2 |
| GRO alpha | 1.9 | 5.6 |
| GRO beta | 1.9 | 6.8 |
| IFN-alpha 2 | 3.6 | 10.0 |
| IFN-beta | 2.4 | 7.1 |
| IFN-gamma | 2.3 | 10.9 |
| IL-1 alpha | 5.0 | 10.6 |
| IL-1 beta | 2.8 | 10.1 |
| IL-1ra | 3.6 | 12.4 |
| IL-2 | 3.9 | 9.6 |
| IL-3 | 3.6 | 9.9 |
| IL-4 | 2.4 | 8.4 |
| IL-5 | 1.8 | 8.3 |
| IL-6 | 2.2 | 8.8 |
| IL-7 | 2.8 | 9.6 |
| IL-8 | 3.2 | 84 |
| IL-9 | 6.2 | 10.3 |
| IL-10 | 3.6 | 11.2 |
| IL-12 p70 | 2.6 | 9.8 |
| IL-13 | 2.5 | 8.3 |
| IL-15 | 3.2 | 11.2 |
| IL-17A | 2.5 | 9.8 |
| IL-17E | 3.1 | 8.1 |
| IL-33 | 2.9 | 9.7 |
| IP-10 | 3.3 | 6.6 |
| MCP-1 | 1.8 | 6.3 |
| MIP-1 alpha | 2.9 | 5.7 |
| MIP-1 beta | 1.7 | 7.9 |
| MIP-3 alpha | 3.0 | 9.4 |
| MIP-3 beta | 2.5 | 8.6 |
| PDGF-AA | 2.7 | 7.1 |
| PDGF-AB/BB | 2.2 | 7.4 |
| PD-L1/B7-H1 | 2.7 | 8.3 |
| RANTES | 1.8 | 7.5 |
| TGF-alpha | 4.4 | 9.2 |
| TNF-alpha | 3.0 | 10.3 |
| TNF-beta | 2.2 | 8.0 |
| TRAIL | 2.4 | 7.9 |
| VEGF | 2.9 | 10.1 |
Accuracy
|
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples. |
||||
| Linearity | Spiked Recovery |
|||
|---|---|---|---|---|
| Analyte | N | Average % | N | Average % |
|
*Natural linearity |
||||
| CD40 Ligand | 4 | 131 | 4 | 75 |
| EGF* | 4 | 150 | HE | |
| Eotaxin | 4 | 68 | 4 | 156 |
| FGF basic | 4 | 128 | 4 | 87 |
| Flt-3 Ligand | 4 | 119 | 4 | 78 |
| G-CSF | 4 | 208 | 4 | 34 |
| GM-CSF | 4 | 101 | 4 | 98 |
| Granzyme B | 4 | 107 | 4 | 96 |
| GRO alpha | 4 | 97 | 4 | 139 |
| GRO beta* | 4 | 88 | HE | |
| IFN-alpha 2 | 4 | 106 | 4 | 87 |
| IFN-beta | 4 | 137 | 4 | 63 |
| IFN-gamma | 4 | 127 | 4 | 73 |
| IL-1 alpha | 4 | 143 | 3 | 74 |
| IL-1 beta | 4 | 106 | 4 | 94 |
| IL-1ra* | 4 | 126 | HE | |
| IL-2 | 4 | 115 | 4 | 88 |
| IL-3 | 4 | 123 | 4 | 78 |
| IL-4 | 4 | 141 | 4 | 58 |
| IL-5 | 4 | 113 | 4 | 88 |
| IL-6 | 4 | 126 | 4 | 78 |
| IL-7 | 4 | 110 | 4 | 90 |
| IL-8 | 4 | 118 | 4 | 78 |
| IL-9 | 4 | 111 | 4 | 96 |
| IL-10 | 4 | 117 | 4 | 90 |
| IL-12 p70 | 4 | 111 | 4 | 87 |
| IL-13 | 4 | 118 | 4 | 89 |
| IL-15 | 4 | 103 | 4 | 76 |
| IL-17A | 4 | 128 | 4 | 72 |
| IL-17E | 4 | 150 | 4 | 55 |
| IL-33 | 4 | 103 | 4 | 87 |
| IP-10 | 4 | 124 | 4 | 72 |
| MCP-1* | 4 | 80 | HE | |
| MIP-1 alpha | 4 | 95 | 4 | 111 |
| MIP-1 beta | 4 | 109 | 4 | 95 |
| MIP-3 alpha | 4 | 115 | 4 | 99 |
| MIP-3 beta | 4 | 123 | 4 | 76 |
| PDGF-AA* | 4 | 91 | HE | |
| PDGF-AB/BB* | 4 | 108 | HE | |
| PD-L1/B7-H1 | 4 | 106 | 4 | 96 |
| RANTES* | 4 | 106 | HE | |
| TGF-alpha | 4 | 142 | 4 | 80 |
| TNF-alpha | 4 | 134 | 4 | 71 |
| TNF-beta | 4 | 131 | 4 | 65 |
| TRAIL | 4 | 144 | 4 | 69 |
| VEGF* | 4 | 129 | HE | |
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
Scientific Data
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Human Luminex Cytokine Assay Serum Sample Values Serum samples from 20 apparently healthy individuals were run on the Human XL Cytokine Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.
Product Datasheets
Citations for Human XL Cytokine Luminex® Kit Performance Assay
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Differences in Eotaxin Serum Levels between Polytraumatized Patients with and without Concomitant Traumatic Brain Injury-A Matched Pair Analysis
Authors: Negrin, LL;Ristl, R;Wollner, G;Hajdu, S;
Journal of clinical medicine
Species: Human
Sample Types: Serum
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Helminth exposure and immune response to the two-dose heterologous Ad26.ZEBOV, MVA-BN-Filo Ebola vaccine regimen
Authors: Barry, H;Lhomme, E;Surénaud, M;Nouctara, M;Robinson, C;Bockstal, V;Valea, I;Somda, S;Tinto, H;Meda, N;Greenwood, B;Thiébaut, R;Lacabaratz, C;
PLoS neglected tropical diseases
Species: Human
Sample Types: Serum
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Protocol for a multicentre cross-sectional, longitudinal ambulatory clinical trial in rheumatoid arthritis and Parkinson's disease patients analysing the relation between the gut microbiome, fasting and immune status in Germany (ExpoBiome)
Authors: Hansen, B;Laczny, CC;Aho, VTE;Frachet-Bour, A;Habier, J;Ostaszewski, M;Michalsen, A;Hanslian, E;Koppold, DA;Hartmann, AM;Steckhan, N;Mollenhauer, B;Schade, S;Roomp, K;Schneider, JG;Wilmes, P;
BMJ open
Species: Human
Sample Types: Plasma
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Dissecting human population variation in single-cell responses to SARS-CoV-2
Authors: Aquino, Y;Bisiaux, A;Li, Z;O'Neill, M;Mendoza-Revilla, J;Merkling, SH;Kerner, G;Hasan, M;Libri, V;Bondet, V;Smith, N;de Cevins, C;Ménager, M;Luca, F;Pique-Regi, R;Barba-Spaeth, G;Pietropaoli, S;Schwartz, O;Leroux-Roels, G;Lee, CK;Leung, K;Wu, JT;Peiris, M;Bruzzone, R;Abel, L;Casanova, JL;Valkenburg, SA;Duffy, D;Patin, E;Rotival, M;Quintana-Murci, L;
Nature
Species: Human
Sample Types: Cell Culture Supernates
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First-line durvalumab and tremelimumab with chemotherapy in RAS-mutated metastatic colorectal cancer: a phase 1b/2 trial
Authors: Thibaudin, M;Fumet, JD;Chibaudel, B;Bennouna, J;Borg, C;Martin-Babau, J;Cohen, R;Fonck, M;Taieb, J;Limagne, E;Blanc, J;Ballot, E;Hampe, L;Bon, M;Daumoine, S;Peroz, M;Mananet, H;Derangère, V;Boidot, R;Michaud, HA;Laheurte, C;Adotevi, O;Bertaut, A;Truntzer, C;Ghiringhelli, F;
Nature medicine
Species: Human
Sample Types: Plasma
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Peripheral immune cell death in sepsis based on bulk RNA and single-cell RNA sequencing
Authors: Liu, S;Duan, C;Xie, J;Zhang, J;Luo, X;Wang, Q;Liang, X;Zhao, X;Zhuang, R;Zhao, W;Yin, W;
Heliyon
Species: Human
Sample Types: Plasma
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The workflow for the Luminex is incredibly long (8 hours minimum), but takes only one full day to perform. Before performing Luminex, ensure that your machine has been serviced recently and is in working order, since the variability between samples can be influenced by faulty fluidics.
