Human VIPR2 APC-conjugated Antibody

Catalog #: FAB5416A Datasheet / COA / SDS

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FAB5416A-025

Detection of VIP R2 in HEK293 Human Cell Line Transfected with Human VIP R2 and eGFP by Flow Cytometry.

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All VIPR2 Antibodies

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Human VIPR2 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human VIP R2 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 476031

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human VIP R2 Glu24-Ile438 Accession # P41587

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of VIP R2 in HEK293 Human Cell Line Transfected with Human VIP R2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human VIP R2 and eGFP were stained with either (A) Mouse Anti-Human VIP R2 APC-conjugated Monoclonal Antibody (Catalog # FAB5416A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 degreesC as supplied.

Background: VIPR2

Human VIP R2 (Vasoactive Intestinal Polypeptide Receptor 2), also known as VPAC2 is a 60-70 kDa seven transmembrane glycoprotein receptor that belongs to the G protein coupled receptor 2 family of proteins. The mature chain contains in its first extracellular region a hormone receptor domain (aa 49-114) which includes three N-linked glycosylation sites and a "cilia targeting signal" comprised of and Arg-Asp-Tyr-Arg sequence. Human VIP R2 shares 85% aa sequence identity with mouse and rat VIP R2. In the extracellular domains, human VIP R2 shares 88% and 31% aa sequence identity with mouse VIP R2 and human VIP R1, respectively. VIP R2 is expressed predominantly in skeletal muscle, CD4 + T cells, smooth muscle cells, plasmacytoid dendritic cells, hepatic progenitor cells, monocytes, synoviocytes and select neurons. VIP R1 and VIP R2 bind VIP with equal affinity, but the two receptors are not redundant. VIP R1 interacts with RAMPs; VIP R2 does not. In the dentate gyrus, VIP R1 drives stem cells into a granule cell phenotype while VIP R2 maintains a Nestin + phenotype.

Long Name

Vasoactive Intestinal Peptide Receptor 2

Entrez Gene IDs

7434 (Human); 22355 (Mouse); 29555 (Rat)

Alternate Names

FLJ16511; Helodermin-preferring VIP receptor; PACAP type III receptor; PACAPR3; PACAP-R3; PACAP-R-3; Pituitary adenylate cyclase-activating polypeptide type III receptor; vasoactive intestinal peptide receptor 2; vasoactive intestinal polypeptide receptor 2; VIP R2; VIP2R; VIPR2; VIP-R-2; VPAC2; VPCAP2R