Human VHL Antibody

Catalog #: MAB11435 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11435-100
MAB11435-SP

Detection of Human VHL by Simple WesternTM.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VHL Antibody Summary

Species Reactivity

Human

Specificity

Detects human VHL in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1066402

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human VHL
Asn150-Asp213
Accession # P40337

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

K562 human chronic myelogenous leukemia cells and Daudi human Burkitt's lymphoma cells

Simple Western

20 µg/mL

K562 human chronic myelogenous leukemia cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western

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Detection of Human VHL by Simple Western^TM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cells, loaded at 0.2 mg/mL. A specific band was detected for VHL at approximately 23 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB11435). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Western Blot

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Detection of Human VHL by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cells and Daudi human Burkitt's lymphoma cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB11435) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for VHL at approximately 17, 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VHL

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues. Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others. In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.

References

Kamura T., et al. (1998) Genes & Dev. doi: 10.1101/gad.12.24.3872
Okumura F., et al. (2012) Front. Oncol. doi: 10.3389/fonc.2012.00010
Xie L., et al. (2009) Sci. Signaling doi: 10.1126/scisignal.2000444
Zhang J., et al. (2018) Science doi: 10.1126/science.aap8411

Long Name

Von Hippel-Lindau Tumor Suppressor

Entrez Gene IDs

7428 (Human); 22346 (Mouse)

Alternate Names

elongin binding protein; HRCA1; Protein G7; pVHL; RCA1; VHL; VHL1; von Hippel-Lindau disease tumor suppressor; von Hippel-Lindau syndrome; von Hippel-Lindau tumor suppressor