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Human VEGF-C DuoSet ELISA

Catalog #: DY752B
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY752B
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human VEGF-C ELISA Standard Curve
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Product Details
Citations (13)
FAQs
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human VEGF-C DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
62.5 - 4,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human VEGF-C. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Human VEGF-C ELISA Standard Curve View Larger

Human VEGF-C ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-C

Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

Long Name:
Vascular Endothelial Growth Factor C
Entrez Gene IDs:
7424 (Human); 22341 (Mouse)
Alternate Names:
Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM

Citations for Human VEGF-C DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Harnessing EGLN1 Gene Editing to Amplify HIF-1 alpha and Enhance Human Angiogenic Response
    Authors: Shams, S;Stilhano, RS;Silva, EA;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Cell Culture Supernates
  2. In Vitro Comparison of Lymphangiogenic Potential of Hypoxia Preconditioned Serum (HPS) and Platelet-Rich Plasma (PRP)
    Authors: J Jiang, X Cong, S Alageel, U Dornseifer, AF Schilling, E Hadjipanay, HG Machens, P Moog
    International Journal of Molecular Sciences, 2023-01-19;24(3):.
    Species: Human
    Sample Types: Hypoxia Preconditioned Serum
  3. VEGF-C serum level is associated with response to bevacizumab maintenance therapy in primary ovarian cancer patients
    Authors: Y Ding, L Oliveira-F, E Vettorazzi, K Legler, K Milde-Lang, L Woelber, A Jaeger, K Prieske, V Mueller, B Schmalfeld, S Kuerti
    PLoS ONE, 2022-06-10;17(6):e0269680.
    Species: Human
    Sample Types: Serum
  4. Cytokine Profile and Anti-Inflammatory Activity of a Standardized Conditioned Medium Obtained by Coculture of Monocytes and Mesenchymal Stromal Cells (PRS CK STORM)
    Authors: JP Lapuente, A Blázquez-M, J Marco-Brua, G Gómez, P Desportes, J Sanz, P Fernández, M García-Gil, F Bermejo, JV San Martín, A Algaba, JC De Gregori, D Lapuente, A De Gregori, B Lapuente, MV Andrés, A Anel
    Biomolecules, 2022-03-31;12(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors
    Authors: L Cristinzia, R Poto, G Criscuolo, AL Ferrara, MR Galdiero, L Modestino, S Loffredo, A de Paulis, G Marone, G Spadaro, G Varricchi
    Cells, 2021-01-12;10(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Angiopoietins, Vascular Endothelial Growth Factors and Secretory Phospholipase A2 in Ischemic and Non-Ischemic Heart Failure
    Authors: G Varricchi, S Loffredo, L Bencivenga, AL Ferrara, G Gambino, N Ferrara, A de Paulis, G Marone, G Rengo
    J Clin Med, 2020-06-19;9(6):.
    Species: Human
    Sample Types: Plasma
  7. Superantigenic Activation of Human Cardiac Mast Cells
    Authors: G Varricchi, S Loffredo, F Borriello, A Pecoraro, F Rivellese, A Genovese, G Spadaro, G Marone
    Int J Mol Sci, 2019-04-12;20(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. VEGFC acts as a double-edged sword in renal cell carcinoma aggressiveness
    Authors: PD Ndiaye, M Dufies, S Giuliano, L Douguet, R Grépin, J Durivault, P Lenormand, N Glisse, J Mintcheva, V Vouret-Cra, B Mograbi, M Wurmser, D Ambrosetti, N Rioux-Lecl, P Maire, G Pagès
    Theranostics, 2019-01-21;9(3):661-675.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development
    Authors: J Bourland, J Fradette, FA Auger
    Sci Rep, 2018-09-04;8(1):13191.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Effects of proton versus photon irradiation on (lymph)angiogenic, inflammatory, proliferative and anti-tumor immune responses in head and neck squamous cell carcinoma
    Authors: M Lupu-Plesu, A Claren, S Martial, PD N'Diaye, K Lebrigand, N Pons, D Ambrosetti, I Peyrottes, J Feuillade, J Hérault, M Dufies, J Doyen, G Pagès
    Oncogenesis, 2017-07-03;6(7):e354.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Tuberculosis-diabetes co-morbidity is characterized by heightened systemic levels of circulating angiogenic factors.
    Authors: Kumar N, Moideen K, Sivakumar S, Menon P, Viswanathan V, Kornfeld H, Babu S
    J Infect, 2016-10-04;74(1):10-21.
    Species: Human
    Sample Types: Plasma
  12. Circulating Angiogenic Factors as Biomarkers of Disease Severity and Bacterial Burden in Pulmonary Tuberculosis.
    Authors: Kumar N, Banurekha V, Nair D, Babu S
    PLoS ONE, 2016-01-04;11(1):e0146318.
    Species: Human
    Sample Types: Plasma
  13. The CXCL7/CXCR1/2 axis is a key driver in the growth of clear cell renal cell carcinoma.
    Authors: Grepin R, Guyot M, Giuliano S, Boncompagni M, Ambrosetti D, Chamorey E, Scoazec J, Negrier S, Simonnet H, Pages G
    Cancer Res, 2013-12-12;74(3):873-83.
    Species: Human
    Sample Types: Tissue Culture Supernates

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