Human V1a Vasopressin R/AVPR1A Antibody

Catalog #: MAB10932 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10932-100
MAB10932-SP

Detection of V1a Vasopressin R/AVPR1A in HEK293 Human Cell Line Transfected with Human V1a Vasopressin R/AVPR1A and eGFP by Flow Cytometry.

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Human V1a Vasopressin R/AVPR1A Antibody Summary

Species Reactivity

Human

Specificity

Detects human V1a Vasopressin R/AVPR1A in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 612130

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human V1a Vasopressin R/AVPR1A
Accession # P37288

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human V1a Vasopressin R/AVPR1A and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of V1a Vasopressin R/AVPR1A in HEK293 Human Cell Line Transfected with Human V1a Vasopressin R/AVPR1A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human V1a Vasopressin R/AVPR1A or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human V1a Vasopressin R/AVPR1A Monoclonal Antibody (Catalog # MAB10932) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.

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Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: V1a Vasopressin R/AVPR1A

AVPR1A acts as a receptor for arginine vasopressin and belongs to the subfamily of G protein-coupled receptors which includes AVPR1B, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. AVPR1A mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis. AVPR1A may be involved in social behaviors, including affiliation and attachment.

Long Name

Arginine Vasopressin Receptor 1A

Entrez Gene IDs

552 (Human); 54140 (Mouse); 25107 (Rat)

Alternate Names

Antidiuretic hormone receptor 1a; arginine vasopressin receptor 1A; AVPR V1a; AVPR1; AVPR1A; SCCL vasopressin subtype 1a receptor; V1a Vasopressin R; V1a vasopressin receptor; V1a VasopressinR; V1aR; V1-vascular vasopressin receptor AVPR1A; Vascular/hepatic-type arginine vasopressin receptor; vasopressin V1a receptor

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