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Human Uteroglobin Quantikine ELISA Kit

Catalog #: DUGB00
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DUGB00
Control Products Available
QC181: Quantikine Immunoassay Control Set 665 for Human Uteroglobin
Human Uteroglobin/SCGB1A1 ELISA Saliva/Urine/Human Milk Standard Curve
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Product Details
Procedure
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Uteroglobin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
0.217 ng/mL
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human Uteroglobin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Uteroglobin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Uteroglobin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Uteroglobin and has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human Uteroglobin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Uteroglobin.

Recovery

The recovery of Uteroglobin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 99-109
EDTA Plasma (n=4) 95 85-107
Heparin Plasma (n=4) 95 86-109
Serum (n=4) 95 85-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Uteroglobin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Uteroglobin/SCGB1A1 ELISA Linearity

Scientific Data

N/A Human Uteroglobin/SCGB1A1 ELISA Saliva/Urine/Human Milk Standard Curve View Larger

Human Uteroglobin/SCGB1A1 ELISA Saliva/Urine/Human Milk Standard Curve

N/A Human Uteroglobin/SCGB1A1 ELISA Cellculture Supernate/Serum/Plasma Standard Curve View Larger

Human Uteroglobin/SCGB1A1 ELISA Cellculture Supernate/Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Uteroglobin/SCGB1A1

Uteroglobin is small, secreted protein of the secretoglobin (SCGBs) family. Uteroglobin is expressed by Clara cells, the non-ciliated, non-mucous secretory cells predominant in lung bronchioles, and by other epithelia that communicate with the external environment. Uteroglob in binds to phospholipids and to several cell types, inhibiting inflammation, migration and invasion. It has been reported to bind the lipocalin-1 receptor.

Entrez Gene IDs:
7356 (Human); 22287 (Mouse); 25575 (Rat)
Alternate Names:
Blastokinin; CC10; CC10Urine protein 1; CC16; CCPBP; CCSP; CCSPCCPBP; Clara cell phospholipid-binding protein; Clara cells 10 kDa secretory protein; Clara; PCB-BP; SCGB1A1; Secretoglobin family 1A member 1; secretoglobin, family 1A, member 1 (uteroglobin); UGB; UGBUP-1; UP1; Urinary protein 1; Uteroglobin
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Uteroglobin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Alteration of immune profiles is associated with pulmonary function and symptoms in patients with chronic obstructive pulmonary disease
    Authors: S Li, S Zhao, Z Wu, F Wang, W Li
    Molecular Medicine Reports, 2021-08-26;24(5):.
    Species: Human
    Sample Types: Plasma
  2. Biomarkers of inflammation and epithelial barrier function in multiple sclerosis
    Authors: A Olsson, S Gustavsen, IC Hasselbalc, AR Langkilde, F Sellebjerg, AB Oturai, HB Søndergaar
    Mult Scler Relat Disord, 2020-09-19;46(0):102520.
    Species: Human
    Sample Types: Serum
  3. Club cell protein 16 and cytokeratin fragment 21-1 as early predictors of pulmonary complications in polytraumatized patients with severe chest trauma
    Authors: LL Negrin, G Halat, S Kettner, M Gregori, R Ristl, S Hajdu, T Heinz
    PLoS ONE, 2017-04-05;12(4):e0175303.
    Species: Human
    Sample Types: Serum

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