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Human Ubiquitin Antibody

Catalog #: A-100
6 Citations Datasheet / COA / SDS
Newer Version Available: A-104
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Discontinued Product

A-100 has been discontinued and is replaced by A-104.

Product Details
Citations (6)
FAQs
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ubiquitin Antibody Summary

Species Reactivity
Human
Specificity
The antibody cross-reacts with mouse, rat and human ubiquitin; but not with Nedd8 or Sumo1 proteins. This reagent is non-functional for immunoprecipitation.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Purified human recombinant ubiquitin
Formulation
Supplied as a solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Recommended dilution range for Western blot analysis is 1:500 to 1:1500. At adilution of 1:1000, 10 ng of monomeric ubiquitin can be detected.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Ubiquitin

Rabbit polyclonal antibody with a high specificity and affinity for ubiquitin.  The antibody cross-reacts with free ubiquitin, mono-ubiquitin and poly-ubiquitin chain conjugates.

References
    1. Haas, A.L. and Bright, P.M. (1985) J. Biol. Chem 260:12464.
    2. Guarino, L.A. et al. (1995) Cell  80:301-309.
    3. Takada, K. et al. (1995) Eur. J. Biochem.   233:42.
    Entrez Gene IDs
    7314 (Human); 298693 (Rat)
    Alternate Names
    RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

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    Citations for Human Ubiquitin Antibody

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    6 Citations: Showing 1 - 6
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    1. UBL domain of Usp14 and other proteins stimulates proteasome activities and protein degradation in cells
      Authors: HT Kim, AL Goldberg
      Proc. Natl. Acad. Sci. U.S.A., 2018-11-28;115(50):E11642-E11650.
      Applications: Western Blot
    2. THE PHOSPHODIESTERASE-4 INHIBITOR ROFLUMILAST REVERTS PROTEOLYSIS IN SKELETAL MUSCLE CELLS OF PATIENTS WITH COPD CACHEXIA
      Authors: E Barreiro, E Puig-Vilan, A Salazar-De, S Pascual-Gu, C Casadevall, J Gea
      J. Appl. Physiol., 2018-04-12;0(0):.
      Species: Human
      Sample Types: Cell Lysates
      Applications: Western Blot
    3. The Deubiquitinating Enzyme Usp14 Allosterically Inhibits Multiple Proteasomal Activities and Ubiquitin-Independent Proteolysis
      Authors: HT Kim, AL Goldberg
      J. Biol. Chem., 2017-04-17;0(0):.
      Species: Human
      Sample Types: Protein
      Applications: Western Blot
    4. Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis Thaliana By Promoting Nuclear Proteasome-Mediated HY5 Degradation
      Authors: C Perea-Resa, MA Rodríguez-, E Iniesto, V Rubio, J Salinas
      Mol Plant, 2017-04-12;0(0):.
      Species: Plant
      Sample Types: Protein
      Applications: Western Blot
    5. RNF11 sequestration of the E3 ligase SMURF2 on membranes antagonizes SMAD7 down-regulation of transforming growth factor ? signaling
      Authors: RJ Malonis, W Fu, MJ Jelcic, M Thompson, BS Canter, M Tsikitis, FJ Esteva, I Sánchez
      J. Biol. Chem., 2017-03-14;292(18):7435-7451.
      Applications: Western Blot
    6. Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.
      Authors: Pereira, Marcelo, Baptista, Igor L, Carlassara, Eduardo, Moriscot, Anselmo, Aoki, Marcelo, Miyabara, Elen H
      PLoS ONE, 2014-01-08;9(1):e85283.
      Species: Rat
      Sample Types: Tissue Homogenates
      Applications: Western Blot

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