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Human Trypsin Pan Specific (PRSS1/2/3) Antibody

Catalog #: AF3586
3 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3586
AF3586-SP
Detection of Human Trypsin by Western Blot.
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Product Details
Citations (3)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Trypsin Pan Specific (PRSS1/2/3) Antibody Summary

Specificity
Detects human Trypsin Pan Specific (PRSS1/2/3) in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trypsin 2
Ala16-Ser247
Accession # P07478
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Human pancreas and recombinant human Trypsin 1, 2, and 3
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
12.5 µg/500 µg cell lysate
Human pancreas tissue
Neutralization
Measured by its ability to neutralize Recombinant Human Trypsin 2/PRSS2 (0.02 µg/mL, Catalog # 3586-SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002 ). The Neutralization Dose (ND50) is typically 0.3 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human Trypsin by Western Blot. Western blot shows lysates of human pancreas. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Trypsin at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Western Blot View Larger

Detection of Human Trypsin by Western Blot. Western blot shows recombinant human Trypsin 1, 2, and 3. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Trypsin at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry Trypsin antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

Trypsin in Human Pancreas. Trypsin was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Trypsin antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Trypsin in Human Pancreatic Cancer Tissue. Trypsin was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunoprecipitation View Larger

Detection of Trypsin by Immunoprecipitation Human Trypsin 2/PRSS2 was immunoprecipitated from 500 μg of human pancreas lysates with 12.5 ug Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586). The Trypsin 2/PRSS2-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated human Trypsin 2/PRSS2 was detected by Western blot using 2 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (AF3586) under non-reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trypsin

Trypsin is a general term for any of three 24 kDa gene products that belong to the peptidase S1 family of enzymes. Trypsin in Greek means “rubbing or friction”, and it was chosen here because the first trypsins were extracted from pancreas via a glycerin-based rubbing maceration. Trypsin-1 (cationic), -2 (anionic), and -3 (mesotrypsin) are synthesized as 26 kDa trypsinogens (plus a 35 kDa trypsinogen-3 isoform) that are 247 amino acids (aa) in length. The first 15 aa constitute a signal sequence, followed by an enterokinase-cleavable eight aa propeptide, and a 224 aa mature molecule. Asp194 is linked to enzyme activity, and Tyr154 is sulfated. Over their mature regions, the three trypsins share 84% aa identity.  Mouse trypsin-1 shares 74% aa identity with the human trypsin consensus sequence. Trypsin-1 and -2 cleave peptide bonds carboxylterminal to a Lys or Arg. Trypsin-3 has limited activity on trypsin inhibitors, and thus regulates general trypsin activity. Trypsin-1, -2, and -3 are expressed by multiple tissues, including pancreas (for general digestion), liver, lung and skin. In blood, trypsinogen-1 and -2 circulate at ng/mL concentrations. In pancreatic fluid, trypsin-1, -2, and -3 are found in a 58:40:2 ratio. This antibody did not neutralize the activity of rhTrypsin 1 and rhTrypsin 3 significantly.  

Alternate Names
TRY2; TRY8; TRYP2; Trypsin

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Citations for Human Trypsin Pan Specific (PRSS1/2/3) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models
    Authors: Y Aghazadeh, F Poon, F Sarangi, FTM Wong, ST Khan, X Sun, R Hatkar, BJ Cox, SS Nunes, MC Nostro
    Cell Stem Cell, 2021-09-03;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Single-Cell Transcriptome Profiling Reveals &beta Cell Maturation in Stem Cell-Derived Islets after Transplantation
    Authors: P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
    Cell Rep, 2020-08-25;32(8):108067.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines.
    Authors: Nostro M, Sarangi F, Yang C, Holland A, Elefanty A, Stanley E, Greiner D, Keller G
    Stem Cell Reports, 2015-04-02;4(4):591-604.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human Trypsin Pan Specific (PRSS1/2/3) Antibody

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Human Trypsin Pan Specific (PRSS1/2/3) Antibody
By Anonymous on 04/17/2018
Application: Luminex XMAP technology Sample Tested: Adult pancreas Species: Human

product was used as a primary antibody attached to a solid phase. Works only when combined with Trypsin-1 from another vendor as 50/50 rate

Other Human Trypsin Pan Specific (PRSS1/2/3) Antibody AF3586