Human Total MMP-8 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: MMP-8
The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
Citations for Human Total MMP-8 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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The Clinical Effect of a Propolis and Mangosteen Extract Complex in Subjects with Gingivitis: A Randomized, Double-Blind, and Placebo-Controlled Clinical Trial
Authors: Jung, JS;Choi, GH;Lee, H;Ko, Y;Ji, S;
Nutrients
Species: Human
Sample Types: Gingival Crevicular Fluid
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AdhMMP8 Vector Administration in Muscle: An Alternate Strategy to Regress Hepatic Fibrosis
Authors: García-Bañuelos, J;Oceguera-Contreras, E;Sandoval-Rodríguez, A;Bastidas-Ramírez, BE;Lucano-Landeros, S;Gordillo-Bastidas, D;Gómez-Meda, BC;Santos, A;Cerda-Reyes, E;Armendariz-Borunda, J;
Cells
Species: Rat
Sample Types: Serum, Tissue Homogenates
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Neutrophils and lymphocytes in relation to MMP-8 and MMP-9 levels in pulmonary tuberculosis and HIV co-infection
Authors: B Alisjahban, N Sulastri, R Livia, L Apriani, AJ Verrall, E Sahiratmad
Journal of clinical tuberculosis and other mycobacterial diseases, 2022-03-04;27(0):100308.
Species: Human
Sample Types: Whole Blood
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sTREM-1 Predicts Disease Severity and Mortality in COVID-19 Patients: Involvement of Peripheral Blood Leukocytes and MMP-8 Activity
Authors: PV da Silva-N, JCS de Carvalh, VE Pimentel, MM Pérez, DM Toro, TFC Fraga-Silv, CA Fuzo, CNS Oliveira, LC Rodrigues, JGM Argolo, I Carmona-Ga, NT Neto, COS Souza, TM Fernandes, VAF Bastos, AM Degiovani, LF Constant, FM Ostini, MR Feitosa, RS Parra, FC Vilar, GG Gaspar, JJR da Rocha, O Feres, FG Frantz, RF Gerlach, SR Maruyama, EMS Russo, AL Viana, APM Fernandes, IKFM Santos, VLD Bonato, AL Boechat, A Malheiro, RT Sadikot, M Dias-Baruf, CRB Cardoso, LH Faccioli, CA Sorgi, On Behalf
Viruses, 2021-12-15;13(12):.
Species: Human
Sample Types: Plasma
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Validation and verification of predictive salivary biomarkers for oral health
Authors: N Bostanci, K Mitsakakis, B Afacan, K Bao, B Johannsen, D Baumgartne, L Müller, H Kotolová, G Emingil, M Karpíšek
Scientific Reports, 2021-03-19;11(1):6406.
Species: Human
Sample Types: Saliva
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Activation of a TLR9 mediated innate immune response in preeclampsia
Authors: RD Williamson, FP McCarthy, LC Kenny, CM McCarthy
Sci Rep, 2019-04-11;9(1):5920.
Species: Human
Sample Types: Plasma
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Streptococcus agalactiae Induces Placental Macrophages To Release Extracellular Traps Loaded with Tissue Remodeling Enzymes via an Oxidative Burst-Dependent Mechanism
Authors: RS Doster, JA Sutton, LM Rogers, DM Aronoff, JA Gaddy
MBio, 2018-11-20;9(6):.
Species: Human
Sample Types:
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Association of Matrix Metalloproteinases -7, -8 and -9 and TIMP -1 with Disease Severity in Acute Pancreatitis. A Cohort Study
PLoS ONE, 2016-08-25;11(8):e0161480.
Species: Human
Sample Types: Plasma
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Matrix metalloproteinases and their tissue inhibitors (TIMPs) in Plasmodium falciparum malaria: serum levels of TIMP-1 are associated with disease severity.
Authors: Dietmann A, Helbok R, Lackner P, Issifou S, Lell B, Matsiegui PB, Reindl M, Schmutzhard E, Kremsner PG
J. Infect. Dis., 2008-06-01;197(11):1614-20.
Species: Human
Sample Types: Serum
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