Home / TNF RI/TNFRSF1A / Human TNF RI/TNFRSF1A Antibody

Human TNF RI/TNFRSF1A Antibody

Catalog #: AF225
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF225
AF225-SP
TNF RI/TNFRSF1A in Human Peripheral Blood Lymphocytes.
1 Image
Product Details
Citations (14)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TNF RI/TNFRSF1A Antibody Summary

Species Reactivity
Human
Specificity
Detects human sTNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse TNF sRI and recombinant rat TNF sRI is observed. This anti-human TNF RI antibody can interact with the cell surface 55 kDa human TNF RI and exhibits TNF agonist activities on the A549 human lung carcinoma cell line. This antibody also exhibits TNF agonist activities on the L‑929 mouse fibroblast cell line, suggesting that it has cross-species activity with mouse cell surface TNF RI.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human sTNF RI/TNFRSF1A
Accession # NP_001056
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
Flow Cytometry
2.5 µg/106 cells
Human blood-derived monocytes
Agonist Activity
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 1-6 μg/mL.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TNF RI/TNFRSF1A antibody in Human Peripheral Blood Lymphocytes by Immunocytochemistry (ICC). View Larger

TNF RI/TNFRSF1A in Human Peripheral Blood Lymphocytes. TNF RI/TNFRSF1A was detected in immersion fixed human peripheral blood lymphocytes using 5 µg/mL Human TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF225) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human TNF RI/TNFRSF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. EGFR inhibits TNF-?-mediated pathway by phosphorylating TNFR1 at tyrosine 360 and 401
    Authors: Nam, YW;Shin, JH;Kim, S;Hwang, CH;Lee, CS;Hwang, G;Kim, HR;Roe, JS;Song, J;
    Cell death and differentiation
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. APOL1 promotes endothelial cell activation beyond the glomerulus
    Authors: Miguel Carracedo, Elke Ericson, Rasmus Ågren, Anna Forslöw, Katja Madeyski-Bengtson, Anna Svensson et al.
    iScience
  3. Structural insights into the disruption of TNF-TNFR1 signalling by small molecules stabilising a distorted TNF
    Authors: D McMillan, C Martinez-F, J Porter, D Fox, R Davis, P Mori, T Ceska, B Carrington, A Lawson, T Bourne, J O'Connell
    Nature Communications, 2021-01-25;12(1):582.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. Small molecules that inhibit TNF signalling by stabilising an asymmetric form of the trimer
    Authors: J O'Connell, J Porter, B Kroeplien, T Norman, S Rapecki, R Davis, D McMillan, T Arakaki, A Burgin, D Fox Iii, T Ceska, F Lecomte, A Maloney, A Vugler, B Carrington, BP Cossins, T Bourne, A Lawson
    Nat Commun, 2019-12-19;10(1):5795.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. CAR T Cells Targeting MISIIR for the Treatment of Ovarian Cancer and Other Gynecologic Malignancies
    Authors: A Rodriguez-, P Sharma, M Poussin, AC Boesteanu, NG Minutolo, SB Gitto, DK Omran, MK Robinson, GP Adams, F Simpkins, DJ Powell
    Mol. Ther., 2019-12-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine.
    Authors: Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F
    PLoS ONE, 2015-12-02;10(12):e0143224.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  7. Aspirin Action in Endothelial Cells: Different Patterns of Response Between Chemokine CX3CL1/CX3CR1 and TNF-alpha /TNFR1 Signaling Pathways
    Authors: Dariusz Szukiewicz, Malgorzata Wojciechowska, Anna Bilska, Aleksandra Stangret, Grzegorz Szewczyk, Tarun Kumar Mittal et al.
    Cardiovascular Drugs and Therapy
  8. Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor kappaB (NF-kappaB) signaling.
    Authors: Wang Y, Tan B, Mu R, Chang Y, Wu M, Tu H, Zhang Y, Guo S, Qin X, Li T, Li W, Li A, Zhang X, Li H
    J Biol Chem, 2015-02-13;290(16):10395-405.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  9. EI24 regulates epithelial-to-mesenchymal transition and tumor progression by suppressing TRAF2-mediated NF-kappa B activity
    Authors: Jung-Min Choi, Sushil Devkota, Young Hoon Sung, Han-Woong Lee
    Oncotarget
  10. Pellino3 targets RIP1 and regulates the pro-apoptotic effects of TNF-alpha
    Authors: Shuo Yang, Bingwei Wang, Lisa S. Tang, Jakub Siednienko, John J. Callanan, Paul N. Moynagh
    Nature Communications
  11. Involvement of the Same TNFR1 Residue in Mendelian and Multifactorial Inflammatory Disorders
    Authors: Isabelle Jéru, Serge Charmion, Emmanuelle Cochet, Bruno Copin, Philippe Duquesnoy, Maria Teresa Mitjavila Garcia et al.
    PLoS ONE
  12. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012-02-17;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  13. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression.
    Authors: Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF
    Atherosclerosis, 2006-07-20;192(1):25-32.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human TNF RI/TNFRSF1A Antibody

There are currently no reviews for this product. Be the first to review Human TNF RI/TNFRSF1A Antibody and earn rewards!

Have you used Human TNF RI/TNFRSF1A Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review