Human TNF RI/TNFRSF1A Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB225G
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB225G

Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry.

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Citations (3)

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Human TNF RI/TNFRSF1A Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human TNF RII, recombinant mouse (rm) TNF RI, or rmTNF RII.

Source

Monoclonal Mouse IgG₁ Clone # 16803

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TNF RI/TNFRSF1A
Accession # NP_001056

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB225G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

Long Name

Tumor Necrosis Factor Receptor I

Entrez Gene IDs

7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)

Alternate Names

CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

Citations for Human TNF RI/TNFRSF1A Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection
Authors: F Askarian, S Uchiyama, H Masson, HV Sørensen, O Golten, AC Bunæs, S Mekasha, ÅK Røhr, E Kommedal, JA Ludviksen, MØ Arntzen, B Schmidt, RH Zurich, NM van Sorge, VGH Eijsink, U Krengel, TE Mollnes, NE Lewis, V Nizet, G Vaaje-Kols
Nature Communications, 2021-02-23;12(1):1230.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes
Sci Rep, 2016-09-27;6(0):34310.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
EMBO J., 2012-07-31;31(17):3607-19.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry