Human TMIGD2/CD28H Antibody

Catalog #: MAB8316 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8316-100
MAB8316-SP

Detection of TMIGD2/CD28H in HEK293 Human Cell Line Transfected with Human CD28L/IGPR-1 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TMIGD2/CD28H Antibody Summary

Species Reactivity

Human

Specificity

Detects human TMIGD2/CD28H in direct ELISAs. Stains human TMIGD2/CD28H transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 953728

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line-derived human TMIGD2/CD28H
Met1-Gly150
Accession # Q96BF3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

5-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TMIGD2/CD28H in HEK293 Human Cell Line Transfected with Human CD28L/IGPR-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TMIGD2/CD28H or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB8316) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB004). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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TMIGD2/CD28H in Human Thymus. TMIGD2/CD28H was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB8316) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

References

Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
Parry, R.V. et al. (2003) J.Immunol. 171:166.
Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
Huang, C. et al. (2003) Nature 424:219.
Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

Long Name

Transmembrane And Immunoglobulin Domain Containing 2

Entrez Gene IDs

126259 (Human)

Alternate Names

CD28H; IGPR1; IGPR-1; MGC23244; TMIGD2; transmembrane and immunoglobulin domain containing 2; transmembrane and immunoglobulin domain-containing protein 2