Human TLR3 Antibody

Catalog #: MAB1487
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1487
MAB1487-SP

Detection of Human TLR3 by Western Blot.

2 Images

All TLR3 Antibodies

Product Details

Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLR3 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 512505

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human TLR3
Lys27-Ser711
Accession # Q6PCD4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TLR3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TLR3 Monoclonal Antibody (Catalog # MAB1487) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TLR3 at approximately 116 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Detection of Human TLR3 by Western Blot Knocking-down TLR3 suppresses enhancement of c-IAP2 protein expression by poly(I:C). Western blot analysis of protein extracts from HeLa cells treated with poly(I:C) (500 ng/ml for 16 h) with or without pre-treatment with TLR3-specific and control (NS) siRNAs. The same membrane was stained successively with anti-c-IAP2, TLR3 and beta -actin. Note that the control siRNA by itself enhanced TLR3 and c-IAP2 expression. This is consistent with previous observations and possibly related to the interaction of these small double-strand RNAs with the TLR3 itself regardless of their sequence [36]. In our transfection protocol siRNAs are at 100 nM for the first 4 h and 50 nM for the next 44 h. TLR3 inhibition was not complete with the siRNA n°1 which was apparently weaker than the siRNA n°2. However the siRNA n°1 was strong enough to prevent enhancement of TLR3 and c-IAP2 expression in the absence of poly(I:C). Data are representative of two similar experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20576118), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR3

Human TLR3 is a 116 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules (1, 2). There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (3). The human TLR3 cDNA encodes a 904 amino acid (aa) precursor that contains a 23 aa signal sequence, a 681 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 179 aa cytoplasmic region (4). The horseshoe shaped ECD (5, 6) contains 23 leucine rich repeats, and the cytoplasmic domain contains one Toll/IL-1 receptor (TIR) domain. The ECD of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively. TLR3 is found in phagosomes (7), where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells (8‑11). Ligand binding by TLR3 induces receptor dimerization (5, 6, 8) leading to the release of inflammatory cytokines and dendritic cell maturation (9, 11‑13). TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes (13‑15) and is upregulated by IFN-beta and LPS (9, 14). TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection (12).

References

Sen, G.C. and S.N. Sarkar (2005) Cytokine Growth Factor Rev. 16:1.
Schroder, M. and A.G. Bowie (2005) Trends Immunol. 26:462.
Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588.
Choe, J. et al. (2005) Science 309:581.
Bell, J.K. et al. (2005) Proc. Natl. Acad. Sci. 102:10976.
Nishiya, T. et al. (2002) J. Biol. Chem. 280:37107.
de Bouteiller, O. et al. (2005) J. Biol. Chem. 280:38133.
Alexopoulou, L. et al. (2001) Nature 413:732.
Aksoy, E. et al. (2005) J. Biol. Chem. 280:277.
Kariko, K. et al. (2004) J. Biol. Chem. 279:12542.
Rudd, B.D. et al. (2005) J. Virol. 79:3350.
Farina, C. et al. (2005) J. Neuroimmunol. 159:12.
Heinz, S. et al. (2003) J. Biol. Chem. 278:21502.
Wang, T. et al. (2004) Nat. Med. 10:1366.

Long Name

Toll-like Receptor 3

Entrez Gene IDs

7098 (Human); 142980 (Mouse); 364594 (Rat)

Alternate Names

CD283 antigen; CD283; TLR3; toll-like receptor 3

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COA

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Citations for Human TLR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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