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Human TLR3 Antibody

Catalog #: MAB1487
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1487
MAB1487-SP
Detection of Human TLR3 by Western Blot.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TLR3 in Western blots.
Source
Monoclonal Mouse IgG2b Clone # 512505
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TLR3 antibody by Western Blot. View Larger

Detection of Human TLR3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TLR3 Monoclonal Antibody (Catalog # MAB1487) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TLR3 at approximately 116 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human TLR3 by Western Blot View Larger

Detection of Human TLR3 by Western Blot Knocking-down TLR3 suppresses enhancement of c-IAP2 protein expression by poly(I:C). Western blot analysis of protein extracts from HeLa cells treated with poly(I:C) (500 ng/ml for 16 h) with or without pre-treatment with TLR3-specific and control (NS) siRNAs. The same membrane was stained successively with anti-c-IAP2, TLR3 and beta -actin. Note that the control siRNA by itself enhanced TLR3 and c-IAP2 expression. This is consistent with previous observations and possibly related to the interaction of these small double-strand RNAs with the TLR3 itself regardless of their sequence [36]. In our transfection protocol siRNAs are at 100 nM for the first 4 h and 50 nM for the next 44 h. TLR3 inhibition was not complete with the siRNA n°1 which was apparently weaker than the siRNA n°2. However the siRNA n°1 was strong enough to prevent enhancement of TLR3 and c-IAP2 expression in the absence of poly(I:C). Data are representative of two similar experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20576118), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: TLR3

Human TLR3 is a 116 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules (1, 2). There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (3). The human TLR3 cDNA encodes a 904 amino acid (aa) precursor that contains a 23 aa signal sequence, a 681 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 179 aa cytoplasmic region (4). The horseshoe shaped ECD (5, 6) contains 23 leucine rich repeats, and the cytoplasmic domain contains one Toll/IL-1 receptor (TIR) domain. The ECD of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively. TLR3 is found in phagosomes (7), where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells (8‑11). Ligand binding by TLR3 induces receptor dimerization (5, 6, 8) leading to the release of inflammatory cytokines and dendritic cell maturation (9, 11‑13). TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes (13‑15) and is upregulated by IFN-beta and LPS (9, 14). TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection (12).

References
  1. Sen, G.C. and S.N. Sarkar (2005) Cytokine Growth Factor Rev. 16:1.
  2. Schroder, M. and A.G. Bowie (2005) Trends Immunol. 26:462.
  3. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  4. Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588.
  5. Choe, J. et al. (2005) Science 309:581.
  6. Bell, J.K. et al. (2005) Proc. Natl. Acad. Sci. 102:10976.
  7. Nishiya, T. et al. (2002) J. Biol. Chem. 280:37107.
  8. de Bouteiller, O. et al. (2005) J. Biol. Chem. 280:38133.
  9. Alexopoulou, L. et al. (2001) Nature 413:732.
  10. Aksoy, E. et al. (2005) J. Biol. Chem. 280:277.
  11. Kariko, K. et al. (2004) J. Biol. Chem. 279:12542.
  12. Rudd, B.D. et al. (2005) J. Virol. 79:3350.
  13. Farina, C. et al. (2005) J. Neuroimmunol. 159:12.
  14. Heinz, S. et al. (2003) J. Biol. Chem. 278:21502.
  15. Wang, T. et al. (2004) Nat. Med. 10:1366.
Long Name
Toll-like Receptor 3
Entrez Gene IDs
7098 (Human); 142980 (Mouse); 364594 (Rat)
Alternate Names
CD283 antigen; CD283; TLR3; toll-like receptor 3

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Citations for Human TLR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Thymosin alpha-1 reverses M2 polarization of tumor-associated macrophages during efferocytosis
    Authors: YT Wei, XR Wang, C Yan, F Huang, Y Zhang, X Liu, ZF Wen, XT Sun, Y Zhang, YQ Chen, R Gao, N Pan, LX Wang
    Cancer Research, 2022-05-16;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Increased expression of toll-like receptor 3, an anti-viral signaling molecule, and related genes in Alzheimer's disease brains.
    Authors: Walker D, Tang T, Lue L
    Exp Neurol, 2018-08-01;309(0):91-106.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Stimulation of the toll-like receptor 3 promotes metabolic reprogramming in head and neck carcinoma cells
    Authors: Mathieu Veyrat, Sylvère Durand, Marion Classe, Tanja Matijevic Glavan, Natalie Oker, Nikiforos-Ioannis Kapetanakis et al.
    Oncotarget
  4. Toll-like receptor 3 in Epstein-Barr virus-associated nasopharyngeal carcinomas: consistent expression and cytotoxic effects of its synthetic ligand poly(A:U) combined to a Smac-mimetic
    Authors: Benjamin Vérillaud, Mélanie Gressette, Yannis Morel, Carine Paturel, Philippe Herman, Kwok Wai Lo et al.
    Infectious Agents and Cancer
  5. Rb/E2F1 Regulates the Innate Immune Receptor Toll-Like Receptor 3 in Epithelial Cells
    Authors: Manabu Taura, Mary Ann Suico, Kosuke Koyama, Kensei Komatsu, Rui Miyakita, Chizuru Matsumoto et al.
    Molecular and Cellular Biology
  6. Poly(I:C) induces intense expression of c-IAP2 and cooperates with an IAP inhibitor in induction of apoptosis in cancer cells
    Authors: Luc Friboulet, Claire Gourzones, Sai Wah Tsao, Yannis Morel, Carine Paturel, Stéphane Témam et al.
    BMC Cancer

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