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Human TIM-1/KIM-1/HAVCR Antibody

Catalog #: AF1750
37 Citations 1 Review Datasheet / COA / SDS
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AF1750
AF1750-SP
TIM‑1/KIM‑1/HAVCR in Human Kidney Cancer Tissue.
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Citations (37)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIM‑1/KIM-1/HAVCR in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIM‑1/KIM-1/HAVCR (R&D Systems, Catalog # 1750-TM)
Ser21-Thr288
Accession # Q96D42
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TIM‑1/KIM‑1/HAVCR (Catalog # 1750-TM)
Immunohistochemistry
1-15 µg/mL
See below
Dual RNAscope ISH-IHC
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human kidney

Human TIM-1/KIM-1/HAVCR Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human TIM‑1/KIM‑1/HAVCR Biotinylated Antibody (Catalog # BAF1750)

Standard: Recombinant Human TIM-1/KIM-1/HAVCR His-tag Protein, CF (Catalog # 1750-TM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TIM-1/KIM-1/HAVCR antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

TIM‑1/KIM‑1/HAVCR in Human Kidney Cancer Tissue. TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

In-situ Hybridization View Larger

Detection of TIM‑1/KIM‑1/HAVCR in Human Kidney. Formalin-fixed paraffin-embedded tissue sections of human kidney were probed for TIM1 mRNA (ACD RNAScope Probe, catalog #452148; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human TIM1 polyclonal antibody (R&D Systems catalog # AF1750) at 1ug/mL with 1 hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tubules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also known as KIM-1 and HAVCR) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).

References
  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
  2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
  3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  4. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  5. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
  6. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  7. Umetsu, D.T. et al. (2005) J. Pediatr. Gastroenterol. Nutr. 40:S43.
  8. Gao, P-S. et al. (2005) J. Allergy Clin. Immunol. 115:982.
  9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

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Product Specific Notices

This product is covered by one or more of the following US Patents 7,300,652; 7,041,290; 6,664,385 and other US and foreign patents pending or issued.

Citations for Human TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. TIM1 (HAVCR1) Is Not Essential for Cellular Entry of Either Quasi-enveloped or Naked Hepatitis A Virions
    Authors: A Das, A Hirai-Yuki, O González-L, B Rhein, S Moller-Tan, R Brouillett, L Hensley, I Misumi, W Lovell, JM Cullen, JK Whitmire, W Maury, SM Lemon
    MBio, 2017-09-05;8(5):.
  2. Potent Restriction of Sexual Zika Virus Infection by the Lipid Fraction of Extracellular Vesicles in Semen
    Authors: Ruofan Wang, Germán G. Gornalusse, Yeseul Kim, Urvashi Pandey, Florian Hladik, Lucia Vojtech
    Frontiers in Microbiology
  3. Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development
    Authors: Yi Zheng, Robin Zhexuan Yan, Shiyu Sun, Mutsumi Kobayashi, Lifeng Xiang, Ran Yang et al.
    Cell Stem Cell
  4. TIM-1 Mediates Dystroglycan-Independent Entry of Lassa Virus
    Authors: Rachel B. Brouillette, Elisabeth K. Phillips, Radhika Patel, Wadie Mahauad-Fernandez, Sven Moller-Tank, Kai J. Rogers et al.
    Journal of Virology
  5. Plasma KIM-1 Is Associated with Recurrence Risk after Nephrectomy for Localized Renal Cell Carcinoma: A Trial of the ECOG-ACRIN Research Group (E2805)
    Authors: Wenxin Xu, Mäneka Puligandla, Brian Halbert, Naomi B. Haas, Keith T. Flaherty, Robert G. Uzzo et al.
    Clinical Cancer Research
  6. Interaction between TIM-1 and NPC1 Is Important for Cellular Entry of Ebola Virus
    Authors: Makoto Kuroda, Daisuke Fujikura, Asuka Nanbo, Andrea Marzi, Osamu Noyori, Masahiro Kajihara et al.
    Journal of Virology
  7. Protein Kinase C-δ Mediates Kidney Tubular Injury in Cold Storage–Associated Kidney Transplantation
    Authors: Jiefu Zhu, Gang Zhang, Zhixia Song, Xiaohong Xiang, Shaoqun Shu, Zhiwen Liu et al.
    Journal of the American Society of Nephrology
  8. Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids
    Authors: Jessica M. Vanslambrouck, Sean B. Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil et al.
    bioRxiv
  9. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells
    Authors: Garreta E, Prado P, Tarantino C et al.
    Nature Materials
  10. ATP/ADP biosensor organoids for drug nephrotoxicity assessment
    Authors: Susa K, Kobayashi K, Galichon P et al.
    Frontiers in cell and developmental biology
  11. Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair
    Authors: Gupta N, Matsumoto T, Hiratsuka K et al.
    Science Translational Medicine
  12. The Role of TIM-1 and CD300a in Zika Virus Infection Investigated with Cell-Based Electrical Impedance
    Authors: Oeyen, M;Heymann, CJF;Jacquemyn, M;Daelemans, D;Schols, D;
    Biosensors
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Stepwise developmental mimicry generates proximal-biased kidney organoids
    Authors: Schnell, J;Miao, Z;Achieng, M;Fausto, CC;Wang, V;Kuyper, F;Thornton, ME;Grubbs, B;Kim, J;Lindström, NO;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Organoid
    Applications: Immunohistochemistry
  14. Kidney organoid models reveal cilium-autophagy metabolic axis as a therapeutic target for PKD both in vitro and in vivo
    Authors: Liu, M;Zhang, C;Gong, X;Zhang, T;Lian, MM;Chew, EGY;Cardilla, A;Suzuki, K;Wang, H;Yuan, Y;Li, Y;Naik, MY;Wang, Y;Zhou, B;Soon, WZ;Aizawa, E;Li, P;Low, JH;Tandiono, M;Montagud, E;Moya-Rull, D;Rodriguez Esteban, C;Luque, Y;Fang, M;Khor, CC;Montserrat, N;Campistol, JM;Izpisua Belmonte, JC;Foo, JN;Xia, Y;
    Cell stem cell
    Species: Xenograft
    Sample Types: Organoid
    Applications: Immunohistochemistry
  15. Interaction between hTIM-1 and Envelope Protein Is Important for JEV Infection
    Authors: Liang, Z;Pan, J;Xie, S;Yang, X;Cao, R;
    Viruses
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Single Nucleotide Variants of the Human TIM-1 IgV Domain with Reduced Ability to Promote Viral Entry into Cells
    Authors: T Hattori, T Saito, H Miyamoto, M Kajihara, M Igarashi, A Takada
    Viruses, 2022-09-26;14(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  17. Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery
    Authors: K Hiratsuka, T Miyoshi, KT Kroll, NR Gupta, MT Valerius, T Ferrante, M Yamashita, JA Lewis, R Morizane
    Science Advances, 2022-09-21;8(38):eabq0866.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  18. Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair
    Authors: Gupta N, Matsumoto T, Hiratsuka K et al.
    Science Translational Medicine
  19. T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Functional Entry Factor for Tick-Borne Encephalitis Virus
    Authors: X Zhang, C Liang, H Wang, Z Guo, H Rong, J Pan, W Li, R Pei, X Chen, Z Zhang, XE Zhang, Z Cui
    MBio, 2022-01-25;0(0):e0286021.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. The Phosphatidylserine Receptor TIM-1 Enhances Authentic Chikungunya Virus Cell Entry
    Authors: J Kirui, Y Abidine, A Lenman, K Islam, YD Gwon, L Lasswitz, M Evander, M Bally, G Gerold
    Cells, 2021-07-20;10(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling
    Authors: K Uchimura, H Wu, Y Yoshimura, BD Humphreys
    Cell Reports, 2020-12-15;33(11):108514.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  22. Immortalized stem cell-derived hepatocyte-like cells: An alternative model for studying dengue pathogenesis and therapy
    Authors: K Kongmanas, N Punyadee, K Wasuworawo, A Songjaeng, T Prommool, Y Pewkliang, S Manocheewa, S Thiemmeca, K Sa-Ngiamsu, C Puttikhunt, KF Faull, S Hongeng, P Avirutnan
    PLoS Negl Trop Dis, 2020-11-20;14(11):e0008835.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells
    Authors: Garreta E, Prado P, Tarantino C et al.
    Nature Materials
  24. Dynamic Dystroglycan Complexes Mediate Cell Entry of Lassa Virus
    Authors: A Herrador, C Fedeli, E Radulovic, KP Campbell, H Moreno, G Gerold, S Kunz
    MBio, 2019-03-26;10(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. TIM-1 Promotes Japanese Encephalitis Virus Entry and Infection
    Authors: J Niu, Y Jiang, H Xu, C Zhao, G Zhou, P Chen, R Cao
    Viruses, 2018-11-14;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Human Sertoli cells support high levels of Zika virus replication and persistence
    Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
    Sci Rep, 2018-04-03;8(1):5477.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Urinary kidney injury molecule?1 as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method
    Authors: Y Jin, X Shao, B Sun, C Miao, Z Li, Y Shi
    Mol Med Rep, 2017-01-05;0(0):.
    Species: Rat
    Sample Types: Urine
    Applications: Immunochromatography
  28. Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells
    Nat Protoc, 2016-12-22;12(1):195-207.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  29. Nanoparticle Detection of Urinary Markers for Point-of-Care Diagnosis of Kidney Injury.
    Authors: Chung H, Pellegrini K, Chung J, Wanigasuriya K, Jayawardene I, Lee K, Lee H, Vaidya V, Weissleder R
    PLoS ONE, 2015-07-17;10(7):e0133417.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Development
  30. Characterizing functional domains for TIM-mediated enveloped virus entry.
    Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
    J Virol, 2014-04-02;88(12):6702-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Modeling oxidative injury response in human kidney organoids
    Authors: Przepiorski A, Vanichapol T, Espiritu E Et al.
    Stem Cell Res Ther
  32. Plasma Kidney Injury Molecule 1 in CKD: Findings From the Boston Kidney Biopsy Cohort and CRIC Studies
    Authors: Insa M Schmidt, Anand Srivastava, Venkata Sabbisetti, Gearoid M McMahon, Jiang He, Jing Chen et al.
    American Journal of Kidney Diseases
  33. Guided tissue organization and disease modeling in a kidney tubule array
    Authors: Balajikarthick Subramanian, Oguzhan Kaya, Martin R. Pollak, Gang Yao, Jing Zhou
    Biomaterials
  34. Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids
    Authors: Jessica M. Vanslambrouck, Sean B. Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil et al.
    Nature Communications
  35. Proximal Tubule p53 in Cold Storage/Transplantation-Associated Kidney Injury and Renal Graft Dysfunction
    Authors: Xiaohong Xiang, Jiefu Zhu, Gang Zhang, Zhengwei Ma, Man J. Livingston, Zheng Dong
    Frontiers in Medicine
  36. Blood Kidney Injury Molecule-1 Is a Biomarker of Acute and Chronic Kidney Injury and Predicts Progression to ESRD in Type I Diabetes
    Authors: Venkata S. Sabbisetti, Sushrut S. Waikar, Daniel J. Antoine, Adam Smiles, Chang Wang, Abinaya Ravisankar et al.
    Journal of the American Society of Nephrology
  37. Chikungunya virus entry and infectivity is primarily facilitated through cell line dependent attachment factors in mammalian and mosquito cells
    Authors: Judith Mary Reyes Ballista, Kerri L. Miazgowicz, Marissa D. Acciani, Ariana R. Jimenez, Ryan S. Belloli, Katherine E. Havranek et al.
    Frontiers in Cell and Developmental Biology

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Human TIM-1/KIM-1/HAVCR Antibody
By Anonymous on 12/15/2017
Application: Detection Assay Sample Tested: Recombinant protein Species: Human