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Human Thrombomodulin/BDCA-3 Antibody

Catalog #: AF3947
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3947
AF3947-SP
Detection of Thrombomodulin/BDCA‑3 in THP‑1 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Thrombomodulin/BDCA-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Thrombomodulin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse Thrombomodulin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Thrombomodulin/BDCA-3
Ala19-Ser515
Accession # CAA29045
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Thrombomodulin/THBD/CD141 (Catalog # 3947-PA)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Thrombomodulin/BDCA-3 (Catalog # 3947-PA), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Thrombomodulin/BDCA-3 antibody in THP-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Thrombomodulin/BDCA‑3 in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Sheep Anti-Human Thrombomodulin/BDCA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3947, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Immunohistochemistry Thrombomodulin/BDCA-3 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

Thrombomodulin/BDCA‑3 in Human Placenta. Thrombomodulin/BDCA-3 was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human Thrombomodulin/BDCA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3947) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombomodulin/BDCA-3

Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid (aa) sequence of human THBD (Accession # P07204) predicts a signal peptide (aa 1-18) and a mature chain (aa 19-575) that consists of following domains: C-type lectin (aa 31-169), EGF-like (aa 241-281, aa 284-324, aa 325-363, aa 365-405, aa 404-440, and aa 441-481), transmembrane (aa 516-539) and cytoplasmic (aa 540-575). The R&D Systems rhTHBD consists of aa 19-515, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (1-3). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal aa residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (4). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (5).

References
  1. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
  2. Wu, K.K. et al. (2000) Ann. Med. 32:73.
  3. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
  4. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
  5. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.
Entrez Gene IDs
7056 (Human); 21824 (Mouse); 83580 (Rat)
Alternate Names
BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; Thrombomodulin; TM

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Citation for Human Thrombomodulin/BDCA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Role of thrombomodulin expression on hematopoietic stem cells
    Authors: Sreemanti Basu, Hai Po Helena Liang, Irene Hernandez, Mark Zogg, British Fields, Jennifer May et al.
    Journal of Thrombosis and Haemostasis

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