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Human TGR5/GPBAR1 Antibody

Catalog #: MAB4286
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4286
MAB4286-SP
Detection of TGR5/GPBAR1 in HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TGR5/GPBAR1 Antibody Summary

Species Reactivity
Human
Specificity
Stains human TGR5/GPBAR1 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 409522
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human TGR5/GPBAR1
Met1-Asn330
Accession # Q8TDU6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TGR5/GPBAR1 antibody in HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP antibody by Flow Cytometry. View Larger

Detection of TGR5/GPBAR1 in HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human TGR5/GPBAR1 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TGR5/GPBAR1 Monoclonal Antibody (Catalog # MAB4286) followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on Mouse IgG2BIsotype Control (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGR5/GPBAR1

G protein coupled bile acid receptor 1 (GPBAR1), also called M-BAR, BG37 or TGR5, is a G-protein coupled receptor superfamily member that is a widely expressed cell surface receptor for bile acids. On macrophages, bile acid engagement of GPBAR1 suppresses phagocytosis and other functions. Extracellular portions of human GPBAR1 show approximately 75% amino acid identity with corresponding regions of mouse GPBAR1.

Long Name
Bile Acid Receptor TGR5
Entrez Gene IDs
151306 (Human); 227289 (Mouse); 338443 (Rat)
Alternate Names
BG37; BG37hBG37; G protein-coupled bile acid receptor 1; GPBA; GPBAR1; GPCR19; GPR131; G-protein coupled bile acid receptor 1; G-protein coupled bile acid receptor BG37; G-protein coupled receptor GPCR19; M-BAR; M-BARhGPCR19; membrane bile acid receptor; Membrane-type receptor for bile acids; MGC40597; TGR5; TGR5GPCR

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Citations for Human TGR5/GPBAR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. G Protein-Coupled Bile Acid Receptor TGR5 Activation Inhibits Kidney Disease in Obesity and Diabetes
    Authors: Xiaoxin X. Wang, Michal Herman Edelstein, Uzi Gafter, Liru Qiu, Yuhuan Luo, Evgenia Dobrinskikh et al.
    Journal of the American Society of Nephrology
  2. TGR5 signalling inhibits the production of pro-inflammatory cytokines by in vitro differentiated inflammatory and intestinal macrophages in Crohn's disease.
    Authors: Yoneno K, Hisamatsu T, Shimamura K, Kamada N, Ichikawa R, Kitazume M, Mori M, Uo M, Namikawa Y, Matsuoka K, Sato T, Koganei K, Sugita A, Kanai T, Hibi T
    Immunology, 2013-05-01;139(1):19-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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