Human TAZ/WWTR1 Antibody

Catalog # Availability Size / Price Qty
MAB7210
MAB7210-SP
Detection of Human TAZ/WWTR1 by Western Blot.
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Product Details
Citations (2)
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Supplemental Products
Reviews (1)

Human TAZ/WWTR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TAZ/WWTR1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) YAP or rhYes is observed.
Source
Monoclonal Mouse IgG2B Clone # 672027
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TAZ/WWTR1
Met267-Leu400
Accession # Q9GZV5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TAZ/WWTR1 antibody by Western Blot. View Larger

Detection of Human TAZ/WWTR1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TAZ/WWTR1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry TAZ/WWTR1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

TAZ/WWTR1 in BG01V Human Embryonic Stem Cells. TAZ/WWTR1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

TAZ/WWTR1 in A431 Human Cell Line. TAZ/WWTR1 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot Detection of Human TAZ/WWTR1 by Western Blot View Larger

Detection of Human TAZ/WWTR1 by Western Blot Downregulation of CRB3 confers CSC traits on breast cancer cells through the TAZ/ beta -catenin cascade. (a) Western blot detecting the expressions of AMOT, TAZ and beta -catenin. (b) CoIP/western blot analysis showing endogenous AMOT bound to TAZ. (c) Fluorescence-activated cell sorting of CD44high/CD24low population. (d) Western blot of TAZ and stem cell markers. (e, g) Representative images and quantification of formed mammospheres. (f) Western blot of beta -catenin and stem cell markers. XAV939 was used to inhibit beta -catenin. (h) Flow cytometry analysis of the stem cell marker ALDH and the CD44high/CD24low population. All data are presented as mean±s.e.m. and statistical significance was calculated using a two-tailed t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28436991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAZ/WWTR1

TAZ (Transcriptional co-Activatorr with PDZ-binding motif; also WWTR1) is a 50-55 kDa protein that is related to YAP65 TEF-1 interacting protein. It is a widely expressed transcriptional coactivator, and should not be confused with tafazzin/Taz, an enzyme associated with lipid metabolism. TAZ influences the nuclear transport of SMAD-2, -3 and -4, and in the nucleus, TAZ is known to interact with transcription factors such as TFF1, Pax8, NKX2-1 and TEADS, serving as a scaffold for transcriptional activation complexes. Human TAZ is 400 amino acids (aa) in length. It contains one WW domain (aa 124-157) that binds to PPXY motifs, a coiled-coil region (aa 225-259), and a PDZ binding domain (aa 394-400). There are at least four utilized phosphorylation sites. When phosphorylated on Ser89, TAZ preferentially bind to 14-3-3 proteins, promoting its retention in the cytoplasm. Over aa 267-400, human TAZ shares 88% aa identity with mouse TAZ.

Long Name
Transcriptional Coactivator with PDZ-binding Motif
Entrez Gene IDs
25937 (Human); 97064 (Mouse); 295062 (Rat)
Alternate Names
FLJ27004; FLJ45718; TAZ; TAZDKFZp586I1419; transcriptional co-activator with PDZ-binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain containing transcription regulator 1; WW domain-containing transcription regulator protein 1; WWTR1

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Citations for Human TAZ/WWTR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. PYK2 is involved in premalignant acinar cell reprogramming and pancreatic ductal adenocarcinoma maintenance by phosphorylatingβ-cateninY654
    Authors: C Gao, G Chen, DH Zhang, J Zhang, SF Kuan, W Hu, F Esni, X Gao, JL Guan, E Chu, J Hu
    Cell Mol Gastroenterol Hepatol, 2019-07-19;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. CRB3 downregulation confers breast cancer stem cell traits through TAZ/?-catenin
    Authors: P Li, Y Wang, X Mao, Y Jiang, J Liu, J Li, J Wang, R Wang, J She, J Zhang, J Yang, Y Liu, P Liu
    Oncogenesis, 2017-04-24;6(4):e322.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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Reviews for Human TAZ/WWTR1 Antibody

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Human TAZ/WWTR1 Antibody
By Anonymous on 12/23/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: BG01V human embryonic stem cells Species: Human