Human Syntaxin 6 Biotinylated Antibody

Catalog #: BAF5664 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF5664

1 Image

All Syntaxin 6 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Syntaxin 6 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Syntaxin 6 antibodies are ideal for immunocytochemistry colocalization studies in trans-Golgi and endosomal membranes. The unconjugated antibody detects human Syntaxin 6 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross‑reactivity with recombinant human (rh) Syntaxin 8, rhSyntaxin 12, and rhSyntaxin 16 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Syntaxin 6
Ser69-Asn217
Accession # O43752

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

Syntaxin 6 in HeLa Human Cell Line. Syntaxin 6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Syntaxin 6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5664) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to Golgi bodies. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syntaxin 6

Syntaxin 6 (STX6) is a 30‑35 kDa member of the syntaxin family of proteins. It is widely expressed, embedded in trans‑Golgi and endosomal membranes, and is associated with a variety of SNARE proteins. STX6 is involved in the regulation of caveolae‑dependent endocytosis, and is crucial for membrane remodeling. Human STX6 is a type IV single‑pass transmembrane protein (very long cytoplasmic N‑terminus) that is 255 amino acids (aa) in length. It contains a coiled‑coil region (aa 41‑74), a t‑SNARE domain (aa 163‑225) that is likely involved in protein‑protein interactions, and a C‑terminal transmembrane sequence. There are two splice variants that are also 255 aa in length, but show variation over aa 69‑73. Over aa 69‑217, human STX6 shares 93% aa identity with mouse STX6.

Entrez Gene IDs

10228 (Human); 58244 (Mouse); 60562 (Rat)

Alternate Names

STX6; Syntaxin 6; syntaxin-6