Human Syndecan-3 Antibody

Catalog # Availability Size / Price Qty
MAB3539
MAB3539-SP
Product Details
FAQs
Supplemental Products
Reviews

Human Syndecan-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Syndecan-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Syndecan-3, recombinant human (rh) Syndecan-1, or rhSyndecan-2 is observed.
Source
Monoclonal Rat IgG2A Clone # 374420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Syndecan-3
Gln48-Lys383 (predicted)
Accession # O75056
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Syndecan‑3 (Catalog # 3539-SD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-3

Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1‑3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1‑3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of human Syndecan-3 shares 83%, 83%, 92%, 91% and 91% aa identity with of mouse, rat, equine, bovine and canine Syndecan-3, respectively. Splice isoforms of 384 aa and 346 aa, containing either a 28 aa substitution for aa 1‑86 or deletion of aa 1‑96, have been reported (4). Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N-terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120‑150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta, it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11‑13). In adults, it is upregulated during regeneration, such as following myocardial infarction (14).

References
  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
  3. Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
  4. ENTREZ protein Accession # O75056, EAX076736 and EAX07637.
  5. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
  6. Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
  7. Hienola, A. et al. (2006) J. Cell Biol. 174:569.
  8. Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
  9. Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
  10. Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
  11. Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
  12. Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
  13. Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
  14. Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.
Entrez Gene IDs
9672 (Human); 20970 (Mouse)
Alternate Names
KIAA0468; N-Syndecan; SDC3; SDCN; SYND3syndecan proteoglycan 3; syndecan 3 (N-syndecan); syndecan 3; syndecan neural type; Syndecan3; Syndecan-3

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Syndecan-3 Antibody

There are currently no reviews for this product. Be the first to review Human Syndecan-3 Antibody and earn rewards!

Have you used Human Syndecan-3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review