Human Survivin Affinity Purified Polyclonal Ab Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Survivin in Human Breast Cancer Tissue. Survivin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6471) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Human Survivin by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6471) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Survivin at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Survivin by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 24 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6471) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Survivin
Survivin also known as Apoptosis inhibitor survivin and Baculoviral IAP repeat-containing protein 5 (BIRC5) is an 18 kDa member of the inhibitor of apoptosis (IAP) gene family, which encodes negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple Baculovirus IAP repeat (BIR) domains, but this gene encodes seven isoforms with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Survivin is expressed in a cell cycle-dependent manner and associates with the mitotic apparatus.
Product Datasheets
Citations for Human Survivin Affinity Purified Polyclonal Ab
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin
Authors: HANZHANG DONG, GAOGAO LIU, BIAO JIANG, JIUBING GUO, GUOQUAN TAO, WEI YIU et al.
Oncology Letters
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Dendritic cell targeting of survivin protein in a xenogeneic form elicits strong CD4+ T cell immunity to mouse survivin.
Authors: Charalambous A, Oks M, Nchinda G, Yamazaki S, Steinman RM
J. Immunol., 2006-12-15;177(12):8410-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Delta9-tetrahydrocannabinol inhibits cell cycle progression in human breast cancer cells through Cdc2 regulation.
Authors: Caffarel MM, Sarrio D, Palacios J, Guzman M, Sanchez C
Cancer Res., 2006-07-01;66(13):6615-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
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Does Human Survivin Antibody, Catalog # AF6471, detect mouse Survivin?
Homolgy between human and mouse Survivin is ~83%. We have not tested our Human Survivin polyclonal antibody against any mouse samples.
Reviews for Human Survivin Affinity Purified Polyclonal Ab
Average Rating: 5 (Based on 1 Review)
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The antibody MAB886 was used as the capture in a sandwich ELISA along with AF6471 as detection. The ELISA worked very well. Healthy human serum and plasma samples were not detectable although some disease state samples are detectable.
