Human STEAP1 Antibody Summary
Met1-Trp71
Accession # Q9UHE8
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human STEAP1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB5587) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STEAP1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Detection of STEAP1 in HEK293 transfected with Human STEAP1 and eGFP cells by Flow Cytometry. HEK293 transfected with Human STEAP1 and eGFP were stained with eGFP and either (A) Mouse Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB5587) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
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Detection of STEAP1 in LnCaP cells by Flow Cytometry LnCaP cells were stained with Mouse Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB5587, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STEAP1
STEAP1 (six-transmembrane epithelial antigen of the prostate-1) is a 40 kDa protein (predicted) of the STEAP family of metalloreductases. It is expressed mainly at cell‑cell junctions between prostate secretory epithelium, and up‑regulated in prostate and some bladder, colon and ovarian cancers and Ewing’s sarcomas. Human STEAP1 is 339 amino acids (aa) in length. It contains a ferric oxidoreductase domain (aa 119‑265) that includes transmembrane sequences (# 2, 3, 4, and part of 5, out of 6 sequences). Two splice forms diverge at aa 255, terminating after aa 58 or 259. Over aa 1‑71, human and mouse STEAP1 share 68% aa identity.
Product Datasheets
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