Human SOX9 Antibody Summary
Met1-Lys151
Accession # P48436
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human SOX9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO-III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SOX9 at approximately 75 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SOX9 in HEK293 Human Cell Line. SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
SOX9 in BG01V Human Embryonic Stem Cells. SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human SOX9 by Simple WesternTM. Simple Western lane view shows lysates of normal adjacent tissue and Crohns tissue, loaded at 0.2 mg/mL. A specific band was detected for SOX9 at approximately 107 kDa (as indicated) using 20 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse SOX9 by Western Blot Comparison of E12.5 Sox9IE/IE and Sox9FE/FE embryos based on EGFP intensity and Western blotting.(A) Left panel: white light images of E12.5 Sox9IE/IE and Sox9FE/FE embryos; right panel: same embryos imaged by fluorescence microscopy showed a higher EGFP fluorescence in the Sox9FE/FE embryo compared to the Sox9IE/IE embryo, with EGFP expression in Sox9-specific domains in both. (B) Mean overall EGFP fluorescence of E12.5 Sox9IE/IE (13880.04±1015.55; n = 8) and Sox9FE/FE (84579.48±2822.09; n = 4) embryos analyzed by flow cytometry. Overall EGFP fluorescence was calculated by multiplying the MFI and percentage of EGFP-positive cells. Two-tailed Student's T test, ***p<0.0001. (C) Western blot analysis of E12.5 Sox9IE/IE and Sox9FE/FE embryo lysates, resolved on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gels and immunoblotted with anti-Sox9 (upper panel) and anti-GFP (middle panel) antibodies. In the upper panel, Sox9-EGFP fusion protein and Sox9-2A (2A - residual 23 amino acids of F2A) was only detected in the Sox9FE/FE embryo lysate, but not in the CD-1 WT or Sox9IE/IE embryo lysates. Middle panel: Sox9-EGFP fusion protein was not detected in the Sox9FE/FE embryos but EGFP was detected in both the Sox9IE/IE and Sox9FE/FE lysates at predicted molecular weight. CD-1 WT lysate served as negative control for EGFP. Bottom panel: immunoblotting with anti-histone antibody showed equal loading. WT – wild-type. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0028885), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SOX9 by Immunocytochemistry/Immunofluorescence Dbx1‐expressing progenitors give rise to preBötC neurons and glia. A. Confocal images of Dbx1CreERT2; Rosa26tdTomato mouse preBötC sections immunostained for NeuN. Dbx1‐derived neurons (white arrowhead) expressed tdTomato and were immunoreactive for NeuN. Dbx1‐derived glia (gray arrowhead) expressed tdTomato and were not immunoreactive for NeuN. B. Confocal images of Dbx1CreERT2; Rosa26tdTomato mouse preBötC sections 72 h after injection with AAV‐hSyn‐GFP. Dbx1‐derived non‐neuronal cells extend diffuse fibrils that are closely apposed to the outer surface of microvasculature (white arrows). C, Confocal images of Dbx1CreERT2; Rosa26tdTomato mouse preBötC sections immunostained for Sox9. Glia (gray arrowheads) were immunoreactive for Sox9. Dbx1‐derived neurons (white arrowhead) expressed tdTomato but were not immunoreactive for Sox9. Dbx1‐derived glia expressed tdTomato and were immunoreactive for Sox9 (see “overlay” panel). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28611151), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence Feeder-free expansion of SOX9+ BCs. (A) Immunostaining of SOX9+ BCs with anti-P63, anti-KRT5 and anti-SOX9 antibodies. (B) SOX9+ BCs in rugae of 3rd order human airway by anti-SOX9, anti-P63 and anti-CC10 immunostaining. Scale bar, 100 μm. (C) SOX9+ BCs in rugae of 3rd order human airway by anti-KI67 immunostaining. (D) BC colony cultured on feeder-free condition. (E) Karyotyping of cultured BCs. (F) qPCR showing alveolar and bronchial epithelium marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (G) qPCR showing progenitor cell marker (Krt5, P63 and SOX9) gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (H) Western blotting showing marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/proteincell/article/9/3/267/6760074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence Transplantated SOX9+ BCs regenerate functional human lungin vivo. (A) Left, direct fluorescence image under stereomicroscope showing NOD-SCID mouse lung without (upper panel) or with (lower panel) GFP-labeled SOX9+ BC transplantation. Right, cryo-section and direct fluorescence imaging of transplanted GFP-labeled SOX9+ BCs in lung parenchyma. Scale bar, 100 μm. (B) Immunofluorescence imaging of transplanted GFP-labeled SOX9+ BCs in lung parenchyma with human specific Lamin A+C marker costaining. (C) Fully differentiated GFP+ cells lost SOX9 marker expression (arrowhead indicated). Scale bar, 10 μm. (D) Confocal image with human specific Lamin A+C immunostaining (HuLamin) showing regenerated type I (AQP5+) alveolar cells. No type II (SPC+) cells were observed. (E) Confocal image showing regenerated AEC1 (AQP5+ and HOPX+). AQP5 as a membrane-bound protein distributes on surface of GFP+ cells. Arrowheads indicated the overlay of HOPX with GFP signal in nucleus. Scale bar, 20 μm. (F) qPCR with human specific primers showing alveolar and bronchiolar epithelium marker gene expression in SOX9+ BC transplanted chimeric lung (AEC1: AQP5 and HOPX; AEC2: SPB and LAMP3; bronchiolar cells: SCGB1A1 and MUC1). Biological replicates, n = 3. Error bars, S.E.M. (G) Left, clonogenic BCs isolated from human cervix epithelium obtained by biopsy. Right, transplantation of equal numbers of BCs from lung and cervix indicated different incorporation efficiency Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/proteincell/article/9/3/267/6760074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence Feeder-free expansion of SOX9+ BCs. (A) Immunostaining of SOX9+ BCs with anti-P63, anti-KRT5 and anti-SOX9 antibodies. (B) SOX9+ BCs in rugae of 3rd order human airway by anti-SOX9, anti-P63 and anti-CC10 immunostaining. Scale bar, 100 μm. (C) SOX9+ BCs in rugae of 3rd order human airway by anti-KI67 immunostaining. (D) BC colony cultured on feeder-free condition. (E) Karyotyping of cultured BCs. (F) qPCR showing alveolar and bronchial epithelium marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (G) qPCR showing progenitor cell marker (Krt5, P63 and SOX9) gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (H) Western blotting showing marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/proteincell/article/9/3/267/6760074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence Feeder-free expansion of SOX9+ BCs. (A) Immunostaining of SOX9+ BCs with anti-P63, anti-KRT5 and anti-SOX9 antibodies. (B) SOX9+ BCs in rugae of 3rd order human airway by anti-SOX9, anti-P63 and anti-CC10 immunostaining. Scale bar, 100 μm. (C) SOX9+ BCs in rugae of 3rd order human airway by anti-KI67 immunostaining. (D) BC colony cultured on feeder-free condition. (E) Karyotyping of cultured BCs. (F) qPCR showing alveolar and bronchial epithelium marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (G) qPCR showing progenitor cell marker (Krt5, P63 and SOX9) gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (H) Western blotting showing marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/proteincell/article/9/3/267/6760074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human SOX9 Antibody by Immunohistochemistry Evolution of alveolar epithelial gene expression patterns in the developing mouse lung. Sections of E15.5, 16.5, 17.5 and 18.5 wild-type mouse lungs stained for markers of differentiation. (A) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, LPCAT1 (tip cells from E16.5, then AT2 cells). (B) Green, CEBPA (sub-set of tip cells from E16.5, then AT2 cells); red, pro-SFTPC (embryonic epithelium, stronger from E16.5, later specific to AT2 cells). (C) Green, pro-SFTPC (stronger from E16.5, later specific to AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (D) Green, LPCAT1 (tip cells from E16.5, then AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (E) Green, HOPX (stalk cells from E16.5, AT1 cells); red, SOX9 (tip cells); white, E-CAD (epithelial cells). (F) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, HOPX (stalk cells from E16.5, AT1 cells). (G) Green, HOPX (stalk cells from E16.5, AT1 cells); red, LPCAT1 (tip cells from E16.5, then AT2 cells). Arrows, LPCAT1+ HOPX+ cells; arrowheads, LPCAT1+ HOPX− cells. Blue, DAPI (nuclei). Dashed line, edge of lung. Scale bars: 50 μm in A-F, 20 μm in G and insets. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27578791), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human SOX9 Antibody by Immunohistochemistry Glucocorticoid signalling is sufficient, but not essential, to specify alveolar fate. (A) Experimental design: Tomato+ E12.5 or 16.5 tip or stalk was grafted into E12.5 host lung and grown with 50 nM Dx throughout culture. (B) Examples of alveolar-fated tip grafts stained for: green, LPCAT1 (alveolar fate); red, Tomato (graft); white, PDPN (basal and AT1 cells). Arrowheads, PDPN+ AT1 cells. (C) Split bar graph showing results from B. Each type of graft was analysed in at least three independent experiments. (D) E12.5 wild-type lungs were grown with or without Dx for up to 6 days; two independent experimental replicates. Note precocious expression of alveolar markers in the presence of Dx. Lungs cultured without Dx do express LPCAT1 from experimental day 5. Green, LPCAT1 (late tip progenitors and type 2 cells); red, SOX9 (tip progenitors). (E,F) Sections of GR−/− and GR+/+ sibling lungs at E17.5 and E18.5 stained for: green, HOPX (AT1 cells); red, SOX9 (tip progenitors); white, E-CAD (epithelium) (E), and: green, LPCAT1 (late tip progenitors and AT2 cells); red, LAMP3 (AT2 cells); magenta, PDPN (late tip progenitors and AT1 cells) (F). A total of five GR−/− and 5 GR+/+ sibling lungs from three independent litters were observed at both E17.5 and E18.5. Blue, DAPI. Dashed line, edge of lung. Scale bars: 100 μm in B; 50 μm in D-F. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27578791), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human SOX9 Antibody by Immunohistochemistry Evolution of alveolar epithelial gene expression patterns in the developing mouse lung. Sections of E15.5, 16.5, 17.5 and 18.5 wild-type mouse lungs stained for markers of differentiation. (A) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, LPCAT1 (tip cells from E16.5, then AT2 cells). (B) Green, CEBPA (sub-set of tip cells from E16.5, then AT2 cells); red, pro-SFTPC (embryonic epithelium, stronger from E16.5, later specific to AT2 cells). (C) Green, pro-SFTPC (stronger from E16.5, later specific to AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (D) Green, LPCAT1 (tip cells from E16.5, then AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (E) Green, HOPX (stalk cells from E16.5, AT1 cells); red, SOX9 (tip cells); white, E-CAD (epithelial cells). (F) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, HOPX (stalk cells from E16.5, AT1 cells). (G) Green, HOPX (stalk cells from E16.5, AT1 cells); red, LPCAT1 (tip cells from E16.5, then AT2 cells). Arrows, LPCAT1+ HOPX+ cells; arrowheads, LPCAT1+ HOPX− cells. Blue, DAPI (nuclei). Dashed line, edge of lung. Scale bars: 50 μm in A-F, 20 μm in G and insets. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27578791), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human SOX9 Antibody by Immunohistochemistry Glucocorticoid signalling is sufficient, but not essential, to specify alveolar fate. (A) Experimental design: Tomato+ E12.5 or 16.5 tip or stalk was grafted into E12.5 host lung and grown with 50 nM Dx throughout culture. (B) Examples of alveolar-fated tip grafts stained for: green, LPCAT1 (alveolar fate); red, Tomato (graft); white, PDPN (basal and AT1 cells). Arrowheads, PDPN+ AT1 cells. (C) Split bar graph showing results from B. Each type of graft was analysed in at least three independent experiments. (D) E12.5 wild-type lungs were grown with or without Dx for up to 6 days; two independent experimental replicates. Note precocious expression of alveolar markers in the presence of Dx. Lungs cultured without Dx do express LPCAT1 from experimental day 5. Green, LPCAT1 (late tip progenitors and type 2 cells); red, SOX9 (tip progenitors). (E,F) Sections of GR−/− and GR+/+ sibling lungs at E17.5 and E18.5 stained for: green, HOPX (AT1 cells); red, SOX9 (tip progenitors); white, E-CAD (epithelium) (E), and: green, LPCAT1 (late tip progenitors and AT2 cells); red, LAMP3 (AT2 cells); magenta, PDPN (late tip progenitors and AT1 cells) (F). A total of five GR−/− and 5 GR+/+ sibling lungs from three independent litters were observed at both E17.5 and E18.5. Blue, DAPI. Dashed line, edge of lung. Scale bars: 100 μm in B; 50 μm in D-F. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27578791), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human SOX9 Antibody by Immunohistochemistry Evolution of alveolar epithelial gene expression patterns in the developing mouse lung. Sections of E15.5, 16.5, 17.5 and 18.5 wild-type mouse lungs stained for markers of differentiation. (A) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, LPCAT1 (tip cells from E16.5, then AT2 cells). (B) Green, CEBPA (sub-set of tip cells from E16.5, then AT2 cells); red, pro-SFTPC (embryonic epithelium, stronger from E16.5, later specific to AT2 cells). (C) Green, pro-SFTPC (stronger from E16.5, later specific to AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (D) Green, LPCAT1 (tip cells from E16.5, then AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (E) Green, HOPX (stalk cells from E16.5, AT1 cells); red, SOX9 (tip cells); white, E-CAD (epithelial cells). (F) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, HOPX (stalk cells from E16.5, AT1 cells). (G) Green, HOPX (stalk cells from E16.5, AT1 cells); red, LPCAT1 (tip cells from E16.5, then AT2 cells). Arrows, LPCAT1+ HOPX+ cells; arrowheads, LPCAT1+ HOPX− cells. Blue, DAPI (nuclei). Dashed line, edge of lung. Scale bars: 50 μm in A-F, 20 μm in G and insets. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27578791), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX9
SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.
Product Datasheets
Citations for Human SOX9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice
Authors: O'Shea TM, Ao Y, Wang S et al.
Nature Communications
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A bioengineered niche promotes in vivo engraftment and maturation of pluripotent stem cell derived human lung organoids
Authors: Briana R Dye, Priya H Dedhia, Alyssa J Miller, Melinda S Nagy, Eric S White, Lonnie D Shea et al.
eLife
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Protocol for the purification and transcriptomic analysis of mouse astrocytes using GFAT
Authors: Lara Labarta-Bajo, James Deng, Minerva Contreras, Nicola J. Allen
STAR Protoc
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Anabolic phenotype in cartilage-specific mitogen-inducible gene-6 knockout mice is independent of transforming growth factor-alpha
Authors: Ermina Hadzic, Bethia To, Michael A. Pest, Ling Qin, Frank Beier
Osteoarthritis and Cartilage Open
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Generation of Complete Multi−Cell Type Lung Organoids From Human Embryonic and Patient‐Specific Induced Pluripotent Stem Cells for Infectious Disease Modeling and Therapeutics Validation
Authors: Sandra L. Leibel, Rachael N. McVicar, Alicia M. Winquist, Walter D. Niles, Evan Y. Snyder
Current Protocols in Stem Cell Biology
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Microglia contribute to normal myelinogenesis and to oligodendrocyte progenitor maintenance during adulthood
Authors: Nora Hagemeyer, Klara-Maria Hanft, Maria-Anna Akriditou, Nicole Unger, Eun S. Park, E. Richard Stanley et al.
Acta Neuropathologica
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Gene expression profiles of Bapx1 expressing FACS sorted cells from wildtype and Bapx1-EGFP null mouse embryos
Authors: Sumantra Chatterjee, V. Sivakamasundari, Petra Kraus, Sook Peng Yap, Vibhor Kumar, Shyam Prabhakar et al.
Genomics Data
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Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model
Authors: Zhenqing Liu, Jianfei Chao, Cheng Wang, Guihua Sun, Daniel Roeth, Wei Liu et al.
Cell Reports
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The Structure of the Spinal Cord Ependymal Region in Adult Humans Is a Distinctive Trait among Mammals
Authors: Alejandro Torrillas de la Cal, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Christopher Guy Faulkes, Antonio Jesús Jiménez, Isidre Ferrer et al.
Cells
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Efficient and Controlled Generation of 2D and 3D Bile Duct Tissue from Human Pluripotent Stem Cell-Derived Spheroids
Authors: Lipeng Tian, Abhijeet Deshmukh, Zhaohui Ye, Yoon-Young Jang
Stem Cell Reviews and Reports
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Morphology and development of a novel murine skeletal dysplasia
Authors: Marta Marchini, Elizabeth Silva Hernandez, Campbell Rolian
PeerJ
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The Prrx1 limb enhancer marks an adult subpopulation of injury-responsive dermal fibroblasts
Authors: Joshua D. Currie, Lidia Grosser, Prayag Murawala, Maritta Schuez, Martin Michel, Elly M. Tanaka et al.
Biology Open
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R-SPONDIN2+ mesenchymal cells form the bud tip progenitor niche during human lung development
Authors: Renee F.C. Hein, Joshua H. Wu, Emily M. Holloway, Tristan Frum, Ansley S. Conchola, Yu-Hwai Tsai et al.
Developmental Cell
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Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity
Authors: Akitoshi Hara, Katsuhiro Kato, Toshikazu Ishihara, Hiroki Kobayashi, Naoya Asai, Shinji Mii et al.
Genes to Cells
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Choline kinase beta is required for normal endochondral bone formation
Authors: Zhuo Li, Gengshu Wu, Roger B. Sher, Zohreh Khavandgar, Martin Hermansson, Gregory A. Cox et al.
Biochimica et Biophysica Acta (BBA) - General Subjects
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Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells
Authors: Rore H, Owen N, Pina-Aguilar Re Et Al.
Communications biology
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A new genetic strategy for targeting microglia in development and disease
Authors: McKinsey GL, Lizama CO, Keown-Lang AE et al.
Elife
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Sox9 regulates melanocytic fate decision of adult hair follicle stem cells
Authors: Isabel Stüfchen, Felix Beyer, Sebastian Staebler, Stefan Fischer, Melanie Kappelmann, Ruth Beckervordersandforth et al.
iScience
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Charting human development using a multi-endodermal organ atlas and organoid models
Authors: Qianhui Yu, Umut Kilik, Emily M. Holloway, Yu-Hwai Tsai, Christoph Harmel, Angeline Wu et al.
Cell
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An Investigation Into the Role of Osteocalcin in Human Arterial Smooth Muscle Cell Calcification
Authors: Sophie A. Millar, Stephen G. John, Christopher W. McIntyre, Vera Ralevic, Susan I. Anderson, Saoirse E. O'Sullivan
Front Endocrinol (Lausanne)
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Generation of human embryonic stem cell-derived lung organoids
Authors: Le Han, Shanshan Zhao, Fuxun Yu, Zhili Rong, Ying Lin, Yong Chen
STAR Protocols
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Primary Cilia Direct Murine Articular Cartilage Tidemark Patterning Through Hedgehog Signaling and Ambulatory Load
Authors: Danielle Rux, Kimberly Helbig, Biao Han, Courtney Cortese, Eiki Koyama, Lin Han et al.
Journal of Bone and Mineral Research
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Region-Specific Transcriptional Control of Astrocyte Function Oversees Local Circuit Activities
Authors: Anna Yu-Szu Huang, Junsung Woo, Debosmita Sardar, Brittney Lozzi, Navish A Bosquez Bosquez Huerta, Chia-Ching John Lin et al.
Neuron
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Mutant Lef1 controls Gata6 in sebaceous gland development and cancer
Authors: Bénédicte Oulès, Emanuel Rognoni, Esther Hoste, Georgina Goss, Ryan Fiehler, Ken Natsuga et al.
The EMBO Journal
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Fibroblast dedifferentiation as a determinant of successful regeneration
Authors: Tzi-Yang Lin, Tobias Gerber, Yuka Taniguchi-Sugiura, Prayag Murawala, Sarah Hermann, Lidia Grosser et al.
Developmental Cell
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In vitro models of fetal lung development to enhance research into congenital lung diseases
Authors: Soichi Shibuya, Jessica Allen-Hyttinen, Paolo De Coppi, Federica Michielin
Pediatric Surgery International
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beta -catenin and Kras/Foxm1 signaling pathway are critical to restrict Sox9 in basal cells during pulmonary branching morphogenesis
Authors: Vladimir Ustiyan, Yufang Zhang, Anne-Karina T. Perl, Jeffrey A. Whitsett, Tanya V. Kalin, Vladimir V. Kalinichenko
Developmental Dynamics
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Intersections between Regulated Cell Death and Autophagy
Authors: Francesco Napoletano, Olga Baron, Peter Vandenabeele, Bertrand Mollereau, Manolis Fanto
Trends in Cell Biology
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NR5A1 is a novel disease gene for 46,XX testicular and ovotesticular disorders of sex development
Authors: Dorien Baetens, Hans Stoop, Frank Peelman, Anne-Laure Todeschini, Toon Rosseel, Frauke Coppieters et al.
Genetics in Medicine
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Sequential perturbations to mouse corticogenesis following in utero maternal immune activation
Authors: Cesar P Canales, Myka L Estes, Karol Cichewicz, Kartik Angara, John Paul Aboubechara, Scott Cameron et al.
eLife
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Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair
Authors: Gupta N, Matsumoto T, Hiratsuka K et al.
Science Translational Medicine
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Primate cell fusion disentangles gene regulatory divergence in neurodevelopment
Authors: RM Agoglia, D Sun, F Birey, SJ Yoon, Y Miura, K Sabatini, SP Pa?ca, HB Fraser
Nature, 2021-03-17;0(0):.
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Wnts Are Expressed in the Spinal Cord of Adult Mice and Are Differentially Induced after Injury
Authors: Carlos González-Fernández, Carmen María Fernández-Martos, Shannon D. Shields, Ernest Arenas, Francisco Javier Rodríguez
Journal of Neurotrauma
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Digits in a dish: An in vitro system to assess the molecular genetics of hand/foot development at single-cell resolution
Authors: Allison M. Fuiten, Yuki Yoshimoto, Chisa Shukunami, H. Scott Stadler
Frontiers in Cell and Developmental Biology
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Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes
Authors: Chen Jin, Yayu Wu, Haipeng Zhang, Bai Xu, Wenbin Liu, Chunnan Ji et al.
Bioengineering & Translational Medicine
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YAP/TAZ transcriptional co-activators create therapeutic vulnerability to verteporfin in EGFR mutant glioblastoma
Authors: Krishanthan Vigneswaran, Nathaniel H. Boyd, Se-Yeong Oh, Shoeb Lallani, Andrew Boucher, Stewart G. Neill et al.
Clinical Cancer Research
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Periosteal Mesenchymal Progenitor Dysfunction and Extraskeletally‐Derived Fibrosis Contribute to Atrophic Fracture Nonunion
Authors: Luqiang Wang, Robert J Tower, Abhishek Chandra, Lutian Yao, Wei Tong, Zekang Xiong et al.
Journal of Bone and Mineral Research
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Fibrillin-2 is a key mediator of smooth muscle extracellular matrix homeostasis during mouse tracheal tubulogenesis
Authors: Wenguang Yin, Hyun-Taek Kim, ShengPeng Wang, Felix Gunawan, Rui Li, Carmen Buettner et al.
European Respiratory Journal
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Morphogenic, molecular, and cellular adaptations for unidirectional airflow in the chicken lung
Authors: Gerner-Mauro, KN;Ellis, LV;Wang, G;Nayak, R;Lwigale, PY;Poché, RA;Chen, J;
bioRxiv : the preprint server for biology
Species: Chicken, Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Early human fetal lung atlas reveals the temporal dynamics of epithelial cell plasticity
Authors: Quach, H;Farrell, S;Wu, MJM;Kanagarajah, K;Leung, JW;Xu, X;Kallurkar, P;Turinsky, AL;Bear, CE;Ratjen, F;Kalish, B;Goyal, S;Moraes, TJ;Wong, AP;
Nature communications
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Glia-derived adenosine in the ventral hippocampus drives pain-related anxiodepression in a mouse model resembling trigeminal neuralgia
Authors: Lv, XJ;Lv, SS;Wang, GH;Chang, Y;Cai, YQ;Liu, HZ;Xu, GZ;Xu, WD;Zhang, YQ;
Brain, behavior, and immunity
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Genetic Dissection of BDNF and TrkB Expression in Glial Cells
Authors: Niu, C;Yue, X;An, JJ;Bass, R;Xu, H;Xu, B;
Biomolecules
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Ovotesticular cords and ovotesticular follicles: New histologic markers for human ovotesticular syndrome
Authors: Baskin, LS;Cao, M;Li, Y;Baker, L;Cooper, CS;Cunha, GR;
Journal of pediatric urology
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
CEBPA restricts alveolar type 2 cell plasticity during development and injury-repair
Authors: Hassan, D;Chen, J;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Astrocytic ?-catenin signaling via TCF7L2 regulates synapse development and social behavior
Authors: Szewczyk, LM;Lipiec, MA;Liszewska, E;Meyza, K;Urban-Ciecko, J;Kondrakiewicz, L;Goncerzewicz, A;Rafalko, K;Krawczyk, TG;Bogaj, K;Vainchtein, ID;Nakao-Inoue, H;Puscian, A;Knapska, E;Sanders, SJ;Jan Nowakowski, T;Molofsky, AV;Wisniewska, MB;
Molecular psychiatry
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Genetic dissection of BDNF and TrkB expression in glial cells
Authors: Niu, C;Yue, X;An, JJ;Xu, H;Xu, B;
bioRxiv : the preprint server for biology
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
A distal lung organoid model to study interstitial lung disease, viral infection and human lung development
Authors: Matkovic Leko, I;Schneider, RT;Thimraj, TA;Schrode, N;Beitler, D;Liu, HY;Beaumont, K;Chen, YW;Snoeck, HW;
Nature protocols
Species: Human
Sample Types: Organoid
Applications: Immunohistochemistry -
Prenatal triclosan exposure impairs mammalian lung branching morphogenesis through activating Bmp4 signaling
Authors: Q Li, Y Qiao, F Wang, J Zhao, L Wu, H Ge, S Xu
Ecotoxicology and environmental safety, 2023-04-12;256(0):114896.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation
Authors: J Sun, S Osenberg, A Irwin, LH Ma, N Lee, Y Xiang, F Li, YW Wan, IH Park, M Maletic-Sa, N Ballas
Cell Reports, 2023-01-05;42(1):111942.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice
Authors: Y Wang, S Zhang, Z Lan, V Doan, B Kim, S Liu, M Zhu, VL Hull, S Rihani, CL Zhang, JA Gray, F Guo
Cell Reports, 2022-12-20;41(12):111842.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
mTORC1 signaling facilitates differential stem cell differentiation to shape the developing murine lung and is associated with mitochondrial capacity
Authors: K Zhang, E Yao, E Chuang, B Chen, EY Chuang, PT Chuang
Nature Communications, 2022-11-25;13(1):7252.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Maturation and circuit integration of transplanted human cortical organoids
Authors: O Revah, F Gore, KW Kelley, J Andersen, N Sakai, X Chen, MY Li, F Birey, X Yang, NL Saw, SW Baker, ND Amin, S Kulkarni, R Mudipalli, B Cui, S Nishino, GA Grant, JK Knowles, M Shamloo, JR Huguenard, K Deisseroth, SP Pa?ca
Nature, 2022-10-12;610(7931):319-326.
Species: Xenograft
Sample Types: Whole Tissue
Applications: ICC -
The alarmin interleukin-1? triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury
Authors: Bretheau F, Castellanos-Molina A, B�langer D et al.
Nature Communications
-
Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice
Authors: O'Shea TM, Ao Y, Wang S et al.
Nature Communications
-
WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome
Authors: Y Tian, W Wang, S Lautrup, H Zhao, X Li, PWN Law, ND Dinh, EF Fang, HH Cheung, WY Chan
Nature Communications, 2022-09-16;13(1):5456.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
SOX9 reprograms endothelial cells by altering the chromatin landscape
Authors: BM Fuglerud, S Drissler, J Lotto, TL Stephan, A Thakur, R Cullum, PA Hoodless
Nucleic Acids Research, 2022-08-26;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Wnt5a-Vangl1/2 signaling regulates the position and direction of lung branching through the cytoskeleton and focal adhesions
Authors: K Zhang, E Yao, E Chuang, B Chen, EY Chuang, RF Volk, KL Hofmann, B Zaro, PT Chuang
PloS Biology, 2022-08-26;20(8):e3001759.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
SHH and Notch regulate SOX9+ progenitors to govern arcuate POMC neurogenesis
Authors: E Place, E Manning, DW Kim, A Kinjo, G Nakamura, K Ohyama
Frontiers in Neuroscience, 2022-08-11;16(0):855288.
Species: Chicken
Sample Types: Whole Cells
Applications: ICC/IF -
Divergent transcriptional regulation of astrocyte reactivity across disorders
Authors: JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew
Nature, 2022-05-25;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development
Authors: T Gu, D Hao, J Woo, TW Huang, L Guo, X Lin, AG Guzman, A Tovy, C Rosas, M Jeong, Y Zhou, B Deneen, Y Huang, W Li, MA Goodell
Nature Genetics, 2022-05-09;54(5):625-636.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis
Authors: R Belgacemi, S Danopoulos, G Deutsch, I Glass, V Dormoy, S Bellusci, D Al Alam
International Journal of Molecular Sciences, 2022-05-09;23(9):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Specification of CNS macrophage subsets occurs postnatally in defined niches
Authors: T Masuda, L Amann, G Monaco, R Sankowski, O Staszewski, M Krueger, F Del Gaudio, L He, N Paterson, E Nent, F Fernández-, A Yamasaki, M Frosch, M Fliegauf, LFP Bosch, H Ulupinar, N Hagemeyer, D Schreiner, C Dorrier, M Tsuda, C Grothe, A Joutel, R Daneman, C Betsholtz, U Lendahl, KP Knobeloch, T Lämmermann, J Priller, K Kierdorf, M Prinz
Nature, 2022-04-20;604(7907):740-748.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
Age and Alzheimer's Disease-Related Oligodendrocyte Changes in Hippocampal Subregions
Authors: L DeFlitch, E Gonzalez-F, I Crawley, SH Kang
Frontiers in Cellular Neuroscience, 2022-04-07;16(0):847097.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Galectin-1 prevents pathological vascular remodeling in atherosclerosis and abdominal aortic aneurysm
Authors: R Roldán-Mon, JM Pérez-Sáez, I Cerro-Pard, J Oller, D Martinez-L, E Nuñez, SM Maller, C Gutierrez-, N Mendez-Bar, JC Escola-Gil, JB Michel, M Mittelbrun, J Vázquez, LM Blanco-Col, GA Rabinovich, JL Martin-Ven
Science Advances, 2022-03-16;8(11):eabm7322.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting
Authors: AE Lewis, A Kuwahara, J Franzosi, JO Bush
Cell Reports, 2022-03-15;38(11):110510.
Species: Mouse
Sample Types: Whole Tissue
Applications: IF -
Localized astrogenesis regulates gyrification of the cerebral cortex
Authors: Y Shinmyo, K Saito, T Hamabe-Hor, N Kameya, A Ando, K Kawasaki, TAD Duong, M Sakashita, J Roboon, T Hattori, T Kannon, K Hosomichi, M Slezak, MG Holt, A Tajima, O Hori, H Kawasaki
Science Advances, 2022-03-11;8(10):eabi5209.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair
Authors: Gupta N, Matsumoto T, Hiratsuka K et al.
Science Translational Medicine
-
Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia
Authors: T Xu, X Li, Y Guo, E Uhlin, L Holmberg, S Mitra, D Winn, A Falk, E Sundström
EBioMedicine, 2022-02-16;77(0):103882.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Suspension culture promotes serosal mesothelial development in human intestinal organoids
Authors: MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence
Cell Reports, 2022-02-15;38(7):110379.
Species: Human
Sample Types: Organoids
Applications: IHC -
Induction of osteogenesis by bone-targeted Notch activation
Authors: C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams
Elife, 2022-02-04;11(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
DM1 Transgenic Mice Exhibit Abnormal Neurotransmitter Homeostasis and Synaptic Plasticity in Association with RNA Foci and Mis-Splicing in the Hippocampus
Authors: B Potier, L Lallemant, S Parrot, A Huguet-Lac, G Gourdon, P Dutar, M Gomes-Pere
International Journal of Molecular Sciences, 2022-01-06;23(2):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Oligodendrocytes depend on MCL-1 to prevent spontaneous apoptosis and white matter degeneration
Authors: AH Cleveland, A Romero-Mor, LA Azcona, M Herrero, VD Nikolova, S Moy, O Elroy-Stei, V Gama, TR Gershon
Cell Death & Disease, 2021-12-06;12(12):1133.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
IL-33 induces orofacial neuropathic pain through Fyn-dependent phosphorylation of GluN2B in the trigeminal spinal nucleus caudalis
Authors: Y Kimura, Y Hayashi, S Hitomi, D Ikutame, K Urata, I Shibuta, A Sakai, J Ni, K Iwata, M Tonogi, M Shinoda
Brain, Behavior, and Immunity, 2021-10-26;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads
Authors: Belicova L, Repnik U, Delpierre J et al.
Journal of Cell Biology
-
Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture
Authors: M Lattke, R Goldstone, JK Ellis, S Boeing, J Jurado-Arj, N Marichal, JI MacRae, B Berninger, F Guillemot
Nature Communications, 2021-07-15;12(1):4335.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
GluA2 overexpression in oligodendrocyte progenitors promotes postinjury oligodendrocyte regeneration
Authors: RR Khawaja, A Agarwal, M Fukaya, HK Jeong, S Gross, E Gonzalez-F, J Soboloff, DE Bergles, SH Kang
Cell Reports, 2021-05-18;35(7):109147.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Orchestrated cellular, biochemical, and biomechanical optimizations endow platelet-rich plasma-based engineered cartilage with structural and biomechanical recovery
Authors: K Wang, J Li, Y Wang, Y Wang, Y Qin, F Yang, M Zhang, H Zhu, Z Li
Bioactive materials, 2021-04-10;6(11):3824-3838.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury
Authors: C Gong, X Zheng, F Guo, Y Wang, S Zhang, J Chen, X Sun, SZA Shah, Y Zheng, X Li, Y Yin, Q Li, X Huang, T Guo, X Han, SC Zhang, W Wang, H Chen
Cell Reports, 2021-03-23;34(12):108889.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Long-term maturation of human cortical organoids matches key early postnatal transitions
Authors: A Gordon, SJ Yoon, SS Tran, CD Makinson, JY Park, J Andersen, AM Valencia, S Horvath, X Xiao, JR Huguenard, SP Pa?ca, DH Geschwind
Nature Neuroscience, 2021-02-22;0(0):.
Species: Human
Sample Types: Organoid
Applications: IHC -
ApoE-Isoform-Dependent SARS-CoV-2 Neurotropism and Cellular Response
Authors: C Wang, M Zhang, G Garcia, E Tian, Q Cui, X Chen, G Sun, J Wang, V Arumugaswa, Y Shi
Cell Stem Cell, 2021-01-04;28(2):331-342.e5.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk
Authors: SE Howden, SB Wilson, E Groenewege, L Starks, TA Forbes, KS Tan, JM Vanslambro, EM Holloway, YH Chen, S Jain, JR Spence, MH Little
Cell Stem Cell, 2020-12-29;0(0):.
Species: Human
Sample Types: Organoid
Applications: Immunohistochemistry -
ROBO2 signaling in lung development regulates SOX2/SOX9 balance, branching morphogenesis and is dysregulated in nitrofen-induced congenital diaphragmatic hernia
Authors: AN Gonçalves, J Correia-Pi, C Nogueira-S
Respir Res, 2020-11-18;21(1):302.
Species: Rat
Sample Types: Whole Cells, Whole Tissue
Applications: IHC, Western Blot -
Contribution of GATA6 to homeostasis of the human upper pilosebaceous unit and acne pathogenesis
Authors: B Oulès, C Philippeos, J Segal, M Tihy, M Vietri Rud, AM Cujba, PA Grange, S Quist, K Natsuga, L Deschamps, N Dupin, G Donati, FM Watt
Nat Commun, 2020-10-20;11(1):5067.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
DSCAM regulates delamination of neurons in the developing midbrain
Authors: N Arimura, M Okada, S Taya, KI Dewa, A Tsuzuki, H Uetake, S Miyashita, K Hashizume, K Shimaoka, S Egusa, T Nishioka, Y Yanagawa, K Yamakawa, YU Inoue, T Inoue, K Kaibuchi, M Hoshino
Science Advances, 2020-09-02;6(36):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Regional Variation in Epidermal Susceptibility to UV-Induced Carcinogenesis Reflects Proliferative Activity of Epidermal Progenitors
Authors: E Roy, HY Wong, R Villani, T Rouille, B Salik, SL Sim, V Murigneux, MS Stark, JL Fink, HP Soyer, G Walker, JG Lyons, N Saunders, K Khosrotehr
Cell Rep, 2020-06-02;31(9):107702.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Overexpression of MIG-6 in the cartilage induces an osteoarthritis-like phenotype in mice
Authors: M Bellini, MA Pest, M Miranda-Ro, L Qin, JW Jeong, F Beier
Arthritis Res. Ther., 2020-05-19;22(1):119.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Redundant and additive functions of the four Lef/Tcf transcription factors in lung epithelial progenitors
Authors: KN Gerner-Mau, H Akiyama, J Chen
Proc. Natl. Acad. Sci. U.S.A., 2020-05-15;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Differential Proliferation and Maturation of Subcortical Astrocytes During Postnatal Development
Authors: T Shoneye, AT Orrego, R Jarvis, Y Men, MSR Chiang, Y Yang
Front Neurosci, 2020-05-08;14(0):435.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Embryonic microglia influence developing hypothalamic glial populations
Authors: CM Marsters, D Nesan, R Far, N Klenin, QJ Pittman, DM Kurrasch
J Neuroinflammation, 2020-05-06;17(1):146.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Endothelin-1 signaling maintains glial progenitor proliferation in the postnatal subventricular zone
Authors: KL Adams, G Riparini, P Banerjee, M Breur, M Bugiani, V Gallo
Nat Commun, 2020-05-01;11(1):2138.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
iPSC-Derived Intestinal Organoids from Cystic Fibrosis Patients Acquire CFTR Activity upon TALEN-Mediated Repair of the p.F508del Mutation
Authors: A Fleischer, S Vallejo-Dí, JM Martín-Fer, A Sánchez-Gi, M Castresana, A Del Pozo, A Esquisabel, S Ávila, JL Castrillo, E Gaínza, JL Pedraz, M Viñas, D Bachiller
Mol Ther Methods Clin Dev, 2020-04-18;17(0):858-870.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Injured adult neurons regress to an embryonic transcriptional growth state
Authors: GHD Poplawski, R Kawaguchi, E Van Nieker, P Lu, N Mehta, P Canete, R Lie, I Dragatsis, JM Meves, B Zheng, G Coppola, MH Tuszynski
Nature, 2020-04-15;581(7806):77-82.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis
Authors: AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen
Cell Rep, 2020-04-07;31(1):107471.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Individual Limb Muscle Bundles Are Formed through Progressive Steps Orchestrated by Adjacent Connective Tissue Cells during Primary Myogenesis
Authors: L Besse, CJ Sheeba, M Holt, M Labuhn, S Wilde, E Feneck, D Bell, A Kucharska, MPO Logan
Cell Rep, 2020-03-10;30(10):3552-3565.e6.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Temporospatial Expression of Fgfr1 and 2 During Lung Development, Homeostasis, and Regeneration
Authors: T Yuan, K Klinkhamme, H Lyu, S Gao, J Yuan, S Hopkins, JS Zhang, SP De Langhe
Front Pharmacol, 2020-03-02;11(0):120.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Lack of whey acidic protein four disulphide core (WFDC) 2 protease inhibitor causes neonatal death from respiratory failure in mice
Authors: K Nakajima, M Ono, U Radovi?, S Dizdarevi?, SI Tomizawa, K Kuroha, G Naganatsu, I Hoshi, R Matsunaga, T Shirakawa, T Kurosawa, Y Miyazaki, M Seki, Y Suzuki, H Koseki, M Nakamura, T Suda, K Ohbo
Dis Model Mech, 2019-11-12;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Development of glial restricted human neural stem cells for oligodendrocyte differentiation in vitro and in vivo
Authors: S Biswas, SH Chung, P Jiang, S Dehghan, W Deng
Sci Rep, 2019-06-21;9(1):9013.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue
Authors: OC Tysoe, AW Justin, T Brevini, SE Chen, KT Mahbubani, AK Frank, H Zedira, E Melum, K Saeb-Parsy, AE Markaki, L Vallier, F Sampazioti
Nat Protoc, 2019-05-20;14(6):1884-1925.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation
Authors: SG Romeo, KM Alawi, J Rodrigues, A Singh, AP Kusumbe, SK Ramasamy
Nat. Cell Biol., 2019-04-01;21(4):430-441.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Physical positioning markedly enhances brain transduction after intrathecal AAV9 infusion
Authors: MJ Castle, Y Cheng, A Asokan, MH Tuszynski
Sci Adv, 2018-11-14;4(11):eaau9859.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes
Authors: JR Jones, L Kong, MG Hanna, B Hoffman, R Krencik, R Bradley, T Hagemann, J Choi, M Doers, M Dubovis, MA Sherafat, A Bhattachar, C Kendziorsk, A Audhya, A Messing, SC Zhang
Cell Rep, 2018-10-23;25(4):947-958.e4.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling
Authors: Y Zhang, Y Yang, M Jiang, SX Huang, W Zhang, D Al Alam, S Danopoulos, M Mori, YW Chen, R Balasubram, SM Chuva de S, C Serra, M Bialecka, E Kim, S Lin, ALR Toste de C, PN Riccio, WV Cardoso, X Zhang, HW Snoeck, J Que
Cell Stem Cell, 2018-09-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Complex bile duct network formation within liver decellularized extracellular matrix hydrogels
Authors: PL Lewis, J Su, M Yan, F Meng, SS Glaser, GD Alpini, RM Green, B Sosa-Pined, RN Shah
Sci Rep, 2018-08-15;8(1):12220.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus
Authors: JM Rosin, DM Kurrasch
J Neuroinflammation, 2018-06-12;15(1):181.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Does human endometrial LGR5 gene expression suggest the existence of another hormonally regulated epithelial stem cell niche?
Authors: N Tempest, AM Baker, NA Wright, DK Hapangama
Hum. Reprod., 2018-06-01;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Glutamate transporter Slc1a3 mediates inter-niche stem cell activation during skin growth
Authors: B Reichenbac, J Classon, T Aida, K Tanaka, M Genander, C Göritz
EMBO J., 2018-04-03;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors
Authors: II Aigha, B Memon, AK Elsayed, EM Abdelalim
Stem Cell Res Ther, 2018-04-03;9(1):83.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity
Authors: PR Tata, RD Chow, SV Saladi, A Tata, A Konkimalla, A Bara, D Montoro, LP Hariri, AR Shih, M Mino-Kenud, H Mou, S Kimura, LW Ellisen, J Rajagopal
Dev. Cell, 2018-03-26;44(6):679-693.e5.
Species: Human, Mouse
Sample Types: Organoid, Whole Tissue
Applications: IHC-Fr -
Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells
Authors: M Zhang, J Ngo, F Pirozzi, YP Sun, A Wynshaw-Bo
Stem Cell Res Ther, 2018-03-15;9(1):67.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Restorative effects of human neural stem cell grafts on the primate spinal cord
Authors: ES Rosenzweig, JH Brock, P Lu, H Kumamaru, EA Salegio, K Kadoya, JL Weber, JJ Liang, R Moseanko, S Hawbecker, JR Huie, LA Havton, YS Nout-Lomas, AR Ferguson, MS Beattie, JC Bresnahan, MH Tuszynski
Nat. Med., 2018-02-26;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
Authors: B Memon, M Karam, S Al-Khawaga, EM Abdelalim
Stem Cell Res Ther, 2018-01-23;9(1):15.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Meis1 coordinates cerebellar granule cell development by regulating Pax6 transcription, BMP signaling and Atoh1 degradation
Authors: T Owa, S Taya, S Miyashita, M Yamashita, T Adachi, K Yamada, M Yokoyama, S Aida, T Nishioka, YU Inoue, R Goitsuka, T Nakamura, T Inoue, K Kaibuchi, M Hoshino
J. Neurosci., 2018-01-09;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Fate mapping neurons and glia derived from Dbx1-expressing progenitors in mouse preB�tzinger complex
Authors: A Kottick, CA Martin, CA Del Negro
Physiol Rep, 2017-06-01;5(11):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos
Authors: WJ Lee, S Chatterjee, SP Yap, SL Lim, X Xing, P Kraus, W Sun, X Hu, V Sivakamasu, HY Chan, PR Kolatkar, S Prabhakar, T Lufkin
Biomed Res Int, 2017-05-28;2017(0):8932583.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ChIP -
SOX9 is an astrocyte-specific nuclear marker in the adult brain outside the neurogenic regions
Authors: W Sun, A Cornwell, J Li, S Peng, MJ Osorio, NA Su Wanga, A Benraiss, N Lou, SA Goldman, M Nedergaard
J. Neurosci, 2017-03-23;0(0):.
Species: Human, Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC -
YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis
Authors: C Lin, E Yao, K Zhang, X Jiang, S Croll, K Thompson-P, PT Chuang
Elife, 2017-03-21;6(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate
Authors: Emma L Rawlins
Development, 2016-08-30;143(20):3686-3699.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The development and plasticity of alveolar type 1 cells
Authors: J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen
Development, 2015-11-19;143(1):54-65.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.
Authors: Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear C, Post M
Stem Cell Reports, 2015-02-05;4(3):419-30.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column.
Authors: Chatterjee S, Sivakamasundari V, Yap S, Kraus P, Kumar V, Xing X, Lim S, Sng J, Prabhakar S, Lufkin T
BMC Genomics, 2014-12-05;15(0):1072.
Species: Mouse
Sample Types: Chromatin
Applications: ChIP -
Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.
Authors: Zhang X, Westerhoff M, Hart J
Mod Pathol, 2014-11-21;28(5):654-61.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
The hippo pathway effector Yap controls patterning and differentiation of airway epithelial progenitors.
Authors: Mahoney J, Mori M, Szymaniak A, Varelas X, Cardoso W
Dev Cell, 2014-07-17;30(2):137-50.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Two nested developmental waves demarcate a compartment boundary in the mouse lung.
Authors: Alanis, Denise M, Chang, Daniel R, Akiyama, Haruhiko, Krasnow, Mark A, Chen, Jichao
Nat Commun, 2014-05-29;5(0):3923.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
Nature, 2014-03-12;507(7492):376-80.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Pubertal androgenization and gonadal histology in two 46,XY adolescents with NR5A1 mutations and predominantly female phenotype at birth
Authors: M Cools, P Hoebeke, K P Wolffenbuttel, H Stoop, R Hersmus, M Barbaro et al.
European Journal of Endocrinology
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
The vanishing testis: a histomorphologic and clinical assessment.
Authors: Antic T, Hyjek EM, Taxy JB
Am. J. Clin. Pathol., 2011-12-01;136(6):872-80.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Identification of SOX3 as an XX male sex reversal gene in mice and humans.
Authors: Sutton E, Hughes J, White S
J. Clin. Invest., 2010-12-22;121(1):328-41.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
Authors: Cavallo C, Desando G, Facchini A, Grigolo B
J Biomed Mater Res A, 2010-04-01;93(1):86-95.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Cyclic GMP-dependent protein kinase II inhibits cell proliferation, Sox9 expression and Akt phosphorylation in human glioma cell lines.
Authors: Swartling FJ, Ferletta M, Kastemar M, Weiss WA, Westermark B
Oncogene, 2009-06-22;28(35):3121-31.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
A Novel Approach for the Derivation of Putative Primordial Germ Cells and Sertoli Cells from Human Embryonic Stem Cells.
Authors: Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
Stem Cells, 2009-01-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
FOXL2 and SOX9 as parameters of female and male gonadal differentiation in patients with various forms of disorders of sex development (DSD).
Authors: Hersmus R, Kalfa N, de Leeuw B, Stoop H, Oosterhuis JW, de Krijger R, Wolffenbuttel KP, Drop SL, Veitia RA, Fellous M, Jaubert F, Looijenga LH
J. Pathol., 2008-05-01;215(1):31-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Longitudinal in vivo bioimaging of hepatocyte transcription factor activity following cholestatic liver injury in mice
Authors: JM Delhove, SM Buckley, DP Perocheau, R Karda, P Arbuthnot, NC Henderson, SN Waddington, TR McKay
Sci Rep, 2017-02-03;7(0):41874.
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The npBAF to nBAF Chromatin Switch Regulates Cell Cycle Exit in the Developing Mammalian Cortex
Authors: Braun S, Petrova R, Tang J et al.
Genes Dev
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Enhancer Redundancy Allows for Phenotypic Robustness in Mammalian Development
Authors: Marco Osterwalder, Iros Barozzi, Virginie Tissières, Yoko Fukuda-Yuzawa, Brandon J. Mannion, Sarah Y. Afzal et al.
Nature
-
Pubertal androgenization and gonadal histology in two 46,XY adolescents with NR5A1 mutations and predominantly female phenotype at birth
Authors: M Cools, P Hoebeke, K P Wolffenbuttel, H Stoop, R Hersmus, M Barbaro et al.
European Journal of Endocrinology
-
A Na(+)/K(+) ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice
Authors: Marchini M, Ashkin MR, Bellini M Et al.
Front Cell Dev Biol
-
Astrocyte heterogeneity within white matter tracts and a unique subpopulation of optic nerve head astrocytes
Authors: Arpan G. Mazumder, Amélie M. Julé, Paul F. Cullen, Daniel Sun
iScience
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Comparison of IRES and F2A-Based Locus-Specific Multicistronic Expression in Stable Mouse Lines
Authors: Hsiao Yun Chan, Sivakamasundari Sivakamasundari, Xing Xing, Petra Kraus, Sook Peng Yap, Patricia Ng et al.
PLoS ONE
-
Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain
Authors: Yorka Muñoz, Francisco Cuevas‐Pacheco, Gaël Quesseveur, Keith K. Murai
Journal of Neuroscience Research
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Isl1 Regulation of Nkx2.1 in the Early Foregut Epithelium Is Required for Trachea-Esophageal Separation and Lung Lobation
Authors: Eugene Kim, Ming Jiang, Huachao Huang, Yongchun Zhang, Natalie Tjota, Xia Gao et al.
Developmental Cell
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Detection and quantification of epithelial progenitor cell populations in human healthy and IPF lungs
Authors: N. F. Smirnova, A. C. Schamberger, S. Nayakanti, R. Hatz, J. Behr, O. Eickelberg
Respiratory Research
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Genome wide binding (ChIP-Seq) of murine Bapx1 and Sox9 proteins in vivo and in vitro
Authors: Sumantra Chatterjee, Petra Kraus, V. Sivakamasundari, Sook Peng Yap, Vibhor Kumar, Shyam Prabhakar et al.
Genomics Data
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Experience-dependent myelination following stress is mediated by the neuropeptide dynorphin
Authors: Lindsay A. Osso, Kelsey A. Rankin, Jonah R. Chan
Neuron
-
Lung epithelial branching program antagonizes alveolar differentiation.
Authors: Chang DR et al.
Proc Natl Acad Sci U S A
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Isolation of ferret astrocytes reveals their morphological, transcriptional, and functional differences from mouse astrocytes
Authors: Jureepon Roboon, Tsuyoshi Hattori, Dinh Thi Nguyen, Hiroshi Ishii, Mika Takarada-Iemata, Takayuki Kannon et al.
Frontiers in Cellular Neuroscience
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Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads
Authors: Belicova L, Repnik U, Delpierre J et al.
Journal of Cell Biology
-
The alarmin interleukin-1? triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury
Authors: Bretheau F, Castellanos-Molina A, B�langer D et al.
Nature Communications
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Large-scale death of retinal astrocytes during normal development is non-apoptotic and implemented by microglia
Authors: Vanessa M. Puñal, Caitlin E. Paisley, Federica S. Brecha, Monica A. Lee, Robin M. Perelli, Jingjing Wang et al.
PLOS Biology
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The cortical hem lacks stem cell potential despite expressing SOX9 and HOPX
Authors: Alessia Caramello, Christophe Galichet, Miriam Llorian Sopena, Robin Lovell‐Badge, Karine Rizzoti
Developmental Neurobiology
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Rab6A as a Pan-Astrocytic Marker in Mouse and Human Brain, and Comparison with Other Glial Markers (GFAP, GS, Aldh1L1, SOX9)
Authors: Linda Melzer, Thomas M. Freiman, Amin Derouiche
Cells
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Dentate gyrus development requires a cortical hem-derived astrocytic scaffold
Authors: Alessia Caramello, Christophe Galichet, Karine Rizzoti, Robin Lovell-Badge
eLife
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In the chick embryo, estrogen can induce chromosomally male ZZ left gonad epithelial cells to form an ovarian cortex, which supports oogenesis
Authors: Guioli S, Zhao D, Nandi S et al.
Development
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A branching morphogenesis program governs embryonic growth of the thyroid gland
Authors: Shawn Liang, Ellen Johansson, Guillermo Barila, Daniel L. Altschuler, Henrik Fagman, Mikael Nilsson
Development
-
An essential function for autocrine Hedgehog signaling in epithelial proliferation and differentiation in the trachea
Authors: Yin W, Liontos A, Koepke J et al.
bioRxiv
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frozen section of adult mouse brain
PFA fixed, sectioned, PBS washed, treated for 5 min in cold Methanol. Worked great!
For immunohistochemistry & immunocytochemistry application.