Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

Name: Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
Brand: R&D Systems
SKU: DSHH00
Rating: 5 (1 reviews)

A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

Catalog #: DSHH00
5 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DSHH00

Control Products Available

QC219: Quantikine Immunoassay Control Set 882 for Human Shh-N

Human Sonic Hedgehog/Shh N-Terminus ELISA Standard Curve

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All Sonic Hedgehog/Shh ELISAs

Product Details

Citations (5)

FAQs

Supplemental Products

Reviews (1)

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (34 uL), Serum (34 uL), Heparin Plasma (34 uL)

Sensitivity

3.92 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Sonic Hedgehog/Shh N-Terminus Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Shh-N in cell culture supernates, serum, and heparin plasma. It contains E. coli-expressed recombinant human Shh-N and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Shh-N showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Shh-N.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in thirty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using three lots of components.

Cell Culture Supernates, Serum, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	99.5	291	532	109	318	612
Standard Deviation	5.97	8.15	14.4	10.3	24.5	50.3
CV%	6	2.8	2.7	9.4	7.7	8.2

Recovery

The recovery of human Shh-N spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	108	100-117
Heparin Plasma (n=4)	97	85-117
Serum (n=4)	99	81-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Shh-N were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Scientific Data

N/A

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Human Sonic Hedgehog/Shh N-Terminus ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Sonic Hedgehog/Shh

Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).

Entrez Gene IDs:

6469 (Human); 20423 (Mouse)

Alternate Names:

HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells
Authors: R Dettmer, K Cirksena, J Münchhoff, J Kresse, U Diekmann, I Niwolik, FFR Buettner, O Naujok
Cells, 2020-08-20;9(9):.
Species: Human
Sample Types: Cell Culture Supernates
Cav-1 Ablation in Pancreatic Stellate Cells Promotes Pancreatic Cancer Growth through Nrf2-Induced shh Signaling
Authors: S Shao, T Qin, W Qian, X Li, W Li, L Han, D Zhang, Z Wang, Q Ma, Z Wu, E Wu, J Lei
Oxid Med Cell Longev, 2020-04-20;2020(0):1868764.
Species: Human
Sample Types: Cell Culture Supernates
Microparticles Carrying Sonic Hedgehog Are Increased in Humans with Peripheral Artery Disease
Authors: I Giarretta, I Gatto, M Marcantoni, G Lupi, D Tonello, E Gaetani, D Pitocco, R Iezzi, A Truma, A Porfidia, A Visonà, P Tondi, R Pola
Int J Mol Sci, 2018-12-09;19(12):.
Species: Human
Sample Types: Plasma
High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia
Authors: C Xu, C Zou, M Hussain, W Shi, Y Shao, Z Jiang, X Wu, M Lu, J Wu, Q Xie, Y Ke, F Long, L Tang, X Wu
Mucosal Immunol, 2018-06-04;0(0):.
Species: Human
Sample Types: BALF
Hedgehog signaling contributes to bone cancer pain by regulating sensory neuron excitability in rats
Authors: S Liu, Y Lv, XX Wan, ZJ Song, YP Liu, S Miao, GL Wang, GJ Liu
Mol Pain, 2018-01-01;14(0):1744806918767.
Species: Rat
Sample Types: Tissue Homogenates

FAQs

Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
- A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

View all ELISA FAQs